GENE MARKER DERIVED FROM ECKLONIA CAVA AND DETECTION OF POLYCYCLIC AROMATIC HYDROCARBON

본 발명은 다환 방향족 탄화수소의 노출에 반응하는 감태 (Ecklonia cava) 유래 유전자 마커, 이를 이용 한 다환 방향족 탄화수소를 탐지하는 바이오센서 및 탐지방법에 관한 것이다

Transcriptomic responses in perfluorooctane sulfonate exposed Hydra

We evaluated perfluorooctane sulfonate (PFOS)-induced acute toxicity in Hydra magnipapillata. The median lethal concentration of the animals (LC50) were determined to be 71.0 mg/L and 27.2 mg/L after exposure to PFOS for 24 h and 48 h, respectively. The morphological responses of hydra polyps to a range of exposure time were illustrated. To define the repertoire of Hydra genes responding to acute exposure of PFOS, transcriptome dynamics were examined in a series of exposure time (4 h, 12 h, 24 h, and 48 h) by using DNA microarray which contains about 18,000 singletons. As the results, we obtained 779, 413, 2620, and 2018 differentially expressed gene candidates response to PFOS (300 μg/L) exposure for 4 h, 12 h, 24 h and 48 h, respectively. The functional analysis of these candidate genes is underway. These candidate genes have great potential in use as multi-biomarkers for organic pollutants and provide a clue in mechanistic aspects of PFOS toxicity in aquatic animals. respectively. The morphological responses of hydra polyps to a range of exposure time were illustrated. To define the repertoire of Hydra genes responding to acute exposure of PFOS, transcriptome dynamics were examined in a series of exposure time (4 h, 12 h, 24 h, and 48 h) by using DNA microarray which contains about 18,000 singletons. As the results, we obtained 779, 413, 2620, and 2018 differentially expressed gene candidates response to PFOS (300 μg/L) exposure for 4 h, 12 h, 24 h and 48 h, respectively. The functional analysis of these candidate genes is underway. These candidate genes have great potential in use as multi-biomarkers for organic pollutants and provide a clue in mechanistic aspects of PFOS toxicity in aquatic animals.1

Transcripts level changes in marine medaka fish with acute toxicity of 4-Nonylphenol

Differential gene expression profiling was performed with a cDNA microarray in the liver tissue of the marine medaka fish, Oryzias javanicus, after exposure to 4-Nonylphenol which is used for detergents, pesticides, anti-oxidants in plastics and rubbers. One hundred ninety-five differentially expressed candidate genes were identified. The expression of 107 genes was up-regulated and that of 88 genes was down-regulated. These genes are associated with the Information storage and processing (Translation, ribosomal structure and biogenesis, J; RNA processing and modification, A; Transcription, K; Replication, recombination and repair, L; Chromatin structure and dynamics, B), Cellular processes and signaling (Cell cycle control, cell division, chromosome partitioning, D; Defense mechanisms, V; Signal transduction mechanisms, T; Cell wall/membrane/envelope biogenesis, M; Cytoskeleton, Z; Extracellular structures, W; Intracellular trafficking, secretion, and vesicular transport, U; Posttranslational modification, protein turnover, chaperones, O), Metabolism (Energy production and conversion, C; Carbohydrate transport and metabolism, G; Amino acid transport and metabolism, E; Nucleotide transport and metabolism, F; Coenzyme transport and metabolism, H; Lipid transport and metabolism, I; Inorganic ion transport and metabolism, P; Secondary metabolites biosynthesis, transport and catabolism, Q), Postics and rubbers. One hundred ninety-five differentially expressed candidate genes were identified. The expression of 107 genes was up-regulated and that of 88 genes was down-regulated. These genes are associated with the Information storage and processing (Translation, ribosomal structure and biogenesis, J; RNA processing and modification, A; Transcription, K; Replication, recombination and repair, L; Chromatin structure and dynamics, B), Cellular processes and signaling (Cell cycle control, cell division, chromosome partitioning, D; Defens2

Changes in gene expression profile of marine medaka fish with acute toxicity of 4-Nonylphenol

Differential gene expression profiling was performed with a cDNA microarray in the liver tissue of the marine medaka fish, Oryzias javanicus, after exposure to 4-Nonylphenol which is used for detergents, pesticides, anti-oxidants in plastics and rubbers. One hundred ninety-five differentially expressed candidate genes were identified. The expression of 107 genes was up-regulated and that of 88 genes was down-regulated. These genes are associated with the Information storage and processing (Translation, ribosomal structure and biogenesis, J; RNA processing and modification, A; Transcription, K; Replication, recombination and repair, L; Chromatin structure and dynamics, B), Cellular processes and signaling (Cell cycle control, cell division, chromosome partitioning, D; Defense mechanisms, V; Signal transduction mechanisms, T; Cell wall/membrane/envelope biogenesis, M; Cytoskeleton, Z; Extracellular structures, W; Intracellular trafficking, secretion, and vesicular transport, U; Posttranslational modification, protein turnover, chaperones, O), Metabolism (Energy production and conversion, C; Carbohydrate transport and metabolism, G; Amino acid transport and metabolism, E; Nucleotide transport and metabolism, F; Coenzyme transport and metabolism, H; Lipid transport and metabolism, I; Inorganic ion transport and metabolism, P; Secondary metabolites biosynthesis, transport and catabolism, Q), Postics and rubbers. One hundred ninety-five differentially expressed candidate genes were identified. The expression of 107 genes was up-regulated and that of 88 genes was down-regulated. These genes are associated with the Information storage and processing (Translation, ribosomal structure and biogenesis, J; RNA processing and modification, A; Transcription, K; Replication, recombination and repair, L; Chromatin structure and dynamics, B), Cellular processes and signaling (Cell cycle control, cell division, chromosome partitioning, D; Defens1

Use of Javanese Medaka (Oryzias javanicus) cDNA Microarrays in Endocrine Disrupting Chemical Risk Assessment

Endocrine disrupting activity of environmental chemicals has been one of the major public concerns since it could cause reduction in reproductive success and affect human and wildlife populations. Up to this date, only biomarkers used to detect chemicals’ potential endocrine disruption (estrogenicity) in experimental fish species are vitellogenin (a female-specific glucolipoprotein yolk precursor) and choriogenin (a precursor of egg envelope protein), and this fact motivated us to look for other useful biomarkers to assess endocrine disrupting effects of the environmental chemicals. Furthermore, several reports have shown that fish exposed to environmentally relevant concentrations (up to 100 ng/l) of 17β-estradiol (E2) for the period prior to sex differentiation and the time of sex differentiation caused disruptions in the process of sexual differentiation (oocyte development in genotypic male, significant change of the sex ratio towards the female sex, etc.), and alteration in the egg production pattern in the subsequent adults. This clearly indicated that early life stages of fish development were sensitive to low concentrations of EDC, and such exposure is now known to lead to distinctive pathological endpoints. Therefore, we were interested in the endocrine disruption studies using both adult and embryo of medaka that were exposed by EDCs. Naturally, development of a bioassay tool was important in order to reduce time, cost, and labor of single biomarker- and pathological observation-dependent test methods, thus, we developed adult Javanese medaka cDNA microarrays which contain 2,500 cDNA probes. In this presentation, experiments using these microarrays and EDC-exposed medaka will be introduced in details, and their analyzed results will be discussed as well.1

Differentially expressed genes in perfluorooctane sulfonate exposed Hydra

We evaluated perfluorooctane sulfonate (PFOS)-induced acute toxicity in Hydra magnipapillata. The median lethal concentration of the animals (LC50) were determined to be 71.0 mg/L and 27.2 mg/L after exposure to PFOS for 24 h and 48 h, respectively. The morphological responses of hydra polyps to a range of exposure time were illustrated. To define the repertoire of Hydra genes responding to acute exposure of PFOS, transcriptome dynamics were examined in a series of exposure time (4 h, 12 h, 24 h, and 48 h) by using DNA microarray which contains about 18,000 singletons. As the results, we obtained 216, 103, 881, and 659 differentially expressed gene candidates response to PFOS (300 μg/L) exposure for 4 h, 12 h, 24 h and 48 h, respectively. The functional analysis of these candidate genes is underway. These candidate genes have great potential in use as multi-biomarkers for organic pollutants and provide a clue in mechanistic aspects of PFOS toxicity in aquatic animals. respectively. The morphological responses of hydra polyps to a range of exposure time were illustrated. To define the repertoire of Hydra genes responding to acute exposure of PFOS, transcriptome dynamics were examined in a series of exposure time (4 h, 12 h, 24 h, and 48 h) by using DNA microarray which contains about 18,000 singletons. As the results, we obtained 216, 103, 881, and 659 differentially expressed gene candidates response to PFOS (300 μg/L) exposure for 4 h, 12 h, 24 h and 48 h, respectively. The functional analysis of these candidate genes is underway. These candidate genes have great potential in use as multi-biomarkers for organic pollutants and provide a clue in mechanistic aspects of PFOS toxicity in aquatic animals.1

Benzo[a]pyrene responsive genes in Hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same

본 발명은 벤조파이렌(Benzo[a]pyrene, BaP) 노출에 대응하는 히드라(Hydra magnipapillata) 유래의 유전 자 및 이를 이용한 수생태계 환경오염 진단 방법에 관한 것으로, 구체적으로 히드라로부터 유래한 유전자 군 중에서 벤조파이렌의 노출 시간에 따라 특이적으로 발현이 증가 또는 감소하는 유전자들을 확인하였으 므로, 이들은 생체 지표로 이용함으로써 벤조파이렌에 대응하는 히드라 유전자를 마이크로어레이, 이를 이 용한 진단방법 및 상기 마이크로어레이를 포함하는 키트를 해양 생태계의 스트레스 검출 또는 진단에 유용 하게 사용할 수 있다

Sediment pollution evaluation in local waste dumping site by transcriptional alteration in a polychaete species, Perinereis nuntia

This study aimed to examine the biological effects of marine sediment layer in dumping sites using the sediment-dwelling organism at transcription level. The marine sediments were collected from 6 pumping sites, 1 non-dumping site (YBR-001) and 5 dumping sites (YB-009, YB-049, YB-053, YB-098, YB-101) in the Yellow Sea; 5 pumping sites, 1 non-dumping site (DBR-010) and 4 dumping sites (DB-026, DB-079, DB-089, DB-091) in the East Sea. The pore waters were prepared from each sediment sample. The polychaetes were incubated in the pore waters and the expression level of 11 target genes were compared with those of a group exposed to control seawater and the a group exposed to 200 ppb of CdCl2 for 24 hours, respectively. The target genes are 2 metal binding genes (ferritin and hemerythrin-like metalloprotein) and 9 oxidative stress related genes (catalase, glutathione peroxidase 1, glutathione peroxidase 3, glutathione S-transferase-α, glutathione S-transferase- κ, heat shock protein 70, heat shock protein 90α, Mn superoxide dismutase and CuZn superoxide dismutase). Considering the range of gene expression level changes, the degree of sediment pollution could be ranked as YB-053 > 101 > 098 ≥ 049 > YBR-001 in the Yellow Sea; DB-026 > 092 > 079 > 089 in the East Sea.001) and 5 dumping sites (YB-009, YB-049, YB-053, YB-098, YB-101) in the Yellow Sea; 5 pumping sites, 1 non-dumping site (DBR-010) and 4 dumping sites (DB-026, DB-079, DB-089, DB-091) in the East Sea. The pore waters were prepared from each sediment sample. The polychaetes were incubated in the pore waters and the expression level of 11 target genes were compared with those of a group exposed to control seawater and the a group exposed to 200 ppb of CdCl2 for 24 hours, respectively. The target genes are 2 metal binding genes (ferritin and hemerythrin-like metalloprotein) and 9 oxidative stress related genes (catalase, glutathione peroxidase 1, glutathione peroxidase 3,1

ZnO nanoparticles exposure alters transcriptomic profile in Hydra

The acute toxicity test for 20 nm ZnO and 100 nm ZnO and comparative transctriptomic profile analysis using hydra exposed to 20 nm ZnO and 100 nm ZnO were carried out. As a result, the LC50 for 72 hours was 8.7 mg/L in 20 nm ZnO exposure and 14.9 mg/L in 100 nm ZnO exposure group. For the microarray experiment we exposed hydra to 20 nm ZnO and 100 nm ZnO as the concentration of 1/50 of LC50 for 12 hours and hybridized those RNAs extracted from the exposed groups with that of control group on the hydra cDNA chips including 17,639 genes. As the results, 137 genes expressions were induced and 106 genes were reduced over 10 fold by 20 nm ZnO exposure. Among them the expression of multidrug-Resistance like Protein 1 CG6214-PM gene increased over 100-fold. In 100 nm ZnO exposure, the expressions of 137 genes were induced over 10 fold and 106 genes were reduced over 10 fold. The upregulated more than 2-fold in both 20 nm and 100 nm ZnO exposures were 886 genes and most of them belonged to cellular processes and signaling category and especially they were related to signal transduction mechanisms. The downregulated more than 2-fold in both 20 nm and 100 nm ZnO exposures were 1006 genes and most of them were related to signal transduction mechanisms. The functional clustering of differentially expressed genes by ZnO nanoparticle exposure showed that those nanoparticles tend to affect the transcripre and 14.9 mg/L in 100 nm ZnO exposure group. For the microarray experiment we exposed hydra to 20 nm ZnO and 100 nm ZnO as the concentration of 1/50 of LC50 for 12 hours and hybridized those RNAs extracted from the exposed groups with that of control group on the hydra cDNA chips including 17,639 genes. As the results, 137 genes expressions were induced and 106 genes were reduced over 10 fold by 20 nm ZnO exposure. Among them the expression of multidrug-Resistance like Protein 1 CG6214-PM gene increased over 100-fold. In 100 nm ZnO exposure1

Transcriptomic responses in Benzo[a]pyrene exposed Hydra

To define the repertoire of Hydra genes responding to acute exposure of benzo[a]pyrene (BaP), transcriptome dynamics were examined in a series of exposure time (4 h, 12 h, 24 h, and 48 h) by using DNA microarray which contain about 18,000 singletons. During the exposure at nonlethal concentration of BaP (500 μg/L, 1/50 48 h LC50), the drastic changes in transcript level were found between 12 and 24 h. Genes induced or repressed in the early stage of exposure (4 h) included in molecular chaperon, anti-oxidant enzyme, apoptosis, necrosis and cancer related cellular processes. The levels of transcripts which related to development, inflammation, calcium metabolism, Na-K channel, innate immune were affected at 12 h exposure. At the 24 h exposure, the expression level of genes involved in sexual development, neurotransmission, and tumor process were found to be altered. Finally, the expression level of DNA repair, ubiquitination, development, differentiation, tumor and cancer related genes were found to be affected at 48 h exposure. Taken together, the results obtained in differential gene expression profiles at various exposure time offer valuable transcriptomic resources for understanding the biological responses to BaP toxicity.00 singletons. During the exposure at nonlethal concentration of BaP (500 μg/L, 1/50 48 h LC50), the drastic changes in transcript level were found between 12 and 24 h. Genes induced or repressed in the early stage of exposure (4 h) included in molecular chaperon, anti-oxidant enzyme, apoptosis, necrosis and cancer related cellular processes. The levels of transcripts which related to development, inflammation, calcium metabolism, Na-K channel, innate immune were affected at 12 h exposure. At the 24 h exposure, the expression level of genes involved in sexual development, neurotransmission, and tumor process were found to be altered. Finally, the expression level of DNA repair, ubiquitination, develop1