Oligomerization이 호알칼리성 Bacillus sp. I-5가 생산하는 Cyclomaltodextrinase의 역가와 열안정성에 미치는 영향

Thesis(master`s)--서울대학교 대학원 :농생명공학부,2005.A Cyclomaltodextrinase from alkalophilic Bacillus sp. I-5 (CDase I-5) which catalyzes the hydrolysis of cyclomaltodextrin (CD) to maltose was found to be a dodecamer as a supramolecular assembly consisted of six units of dimer. The previous study has shown that the dodecamer dissociates into dimer at pH 6.0 and associates to dodecameric form at pH 7.0. In order to investigate the dissociation/association process in details, CDase I-5 mutant was generated by site-directed mutagenesis in which H49, H89, H539 in N- and C-terminal region were substituted with valine and H547 in C-terminal region was replaced with lysine. The mutant enzymes reached equilibrium state of dodecamer-dimer depending on enzyme concentration at pH 7.0. The dissociation constant (Kd) was estimated to be 1.84¡¿10?28M5. The specific activity of the enzyme was compared between dodecameric and dimeric form after the dimeric form was prepared at different pH condition. The results revealed that dodecamer of wild-type enzyme had three times higher specific activity of hydrolyzing b-CD than dimer. The denaturation enthalpy (¥ÄH) of the wild-type dodecameric enzyme increased two fold and denaturation temperature (Tm) was shifted to high temperature by 16¡É compared with dimer. In case of CDase I-5 mutant enzyme, dodecamer had three times higher specific activity of hydrolyzing b-CD and two times larger ¥ÄH as shown in the wild-type enzyme. Whereas Tm for the mutant enzyme was 5¡É higher compared with that of dimer. Unlike other CD degrading enzymes which have dimeric structure, CDase I-5 have been evolved into supramolecular structure for adaptation to alkaline evironment, thereby enhancing activity and thermostability through oligomerization.Maste

Finite element analysis via parallel multi-level frontal solver based upon MPI

학위논문(석사) - 한국과학기술원 : 기계공학전공, 2002.2, [ v, 54 p. ]한국과학기술원 : 기계공학전공

An exploratory study on the effect of application piracy level selection on mobile platforms

학위논문(석사) - 한국과학기술원 : 경영공학과, 2011.2, [ iv, 34 p. ]한국과학기술원 : 경영공학과

Development of PSE for BT Applications on Computatioanl GRID

funder : 국무조정실I. 제 목 컴퓨팅 그리드 기반의 BT 응용 연구를 위한 PSE 기술개발 II. 연구개발의 목적 및 중요성 컴퓨팅 그리드의 활용을 위해서 BT 응용분야에서 요구되는 사항들을 IT 관점으로 정리하여 핵심이 되는 문제를 추출하고, BT 응용분야에서 수행되는 계산과정을 그리드 상에서 효율적으로 처리할 수 있는 워크플로우 기반의 Problem Solving Environment를 구축한다. III. 연구개발의 내용 및 범위 PSE중 Client 및 Server의 S/W에 해당하는 워크플로우 Management 모듈을 개발한다. 세부내용으로는 다음과 같다. 첫째, BT 응용분야를 위한 워크플로우 개념정립 둘째, 워크플로우 Description Format 정의 셋째, 워크플로우 Management 모듈 구현 넷째, BT 응용과제 팀과의 통합 및 테스트 IV. 연구개발결과 현재까지의 본과제를 수행한 연구개발 결과는 다음과 같다 첫째, 워크플로우의 체계적인 분석 및 모델 정립 둘째, Actvie File System 모델 정립 및 구현 셋째, 워크플로우 Manager, Job Preparation Tool 구현 넷째, 건국대와 공동으로 구축중인 Molecular Grid System(MGrid)과의 연동 V. 기 대 효 과 본 과제에서 지원하는 Hight throughput 형태의 다른 그리드 응용과제를 위해서도 개발된 PSE 기술이 적용될 수 있다. 그리고 BT 응용실험의 생산성이 향상될 것으로 생각된다.I. Title Development of PSE for BT Applications on Computational GRID II. Objective of the study and its importance - Extract core problems in IT point of view by analysing requirements of BT application for utilizing computational Grid - Develop PSE based on workflow concept to manage efficiently simulation process of BT applications on computational Grid. III. Content and scope of the study Develop the Workflow Management Module as a client SW of PSE. Details are as the follows: 1. Define work flow concept for BT application 2. Define work flow description format 3. Implement work flow management module 4. Integrate and test the modules with MGrid IV. Result of the study The intermediate results are as the follows: 1. Analysis of work flow and define a well defined model 2. Design and inplement a gluing module called Active File Sysytem 3. Implement work flow manager and job preparation module on the Active File Sysytem 4. Integrate the modules with MGrid(Molecular Simulation Grid) V. Expected Effects The result of this project can be easily extended for other similar Grid applications, ie, high throughput style of computational Grid applications. The research productivity will be improved by using our toolkit