Banding Patterns of Normal Human Chromosomes

To establish a new technique for chromosome banding, 20 human chromosome preparations (10 males and 10 females) were obtained from the medical school volunteers, and were stained with Giemsa-trypsin-EDTA solution. The stained slides showed banding patterns clearly in each chromosome. The bands produced by trypsin-EDTA treatments appeared to be similar to those found in fluoresecent and other karyotypes; and it was confirmed that the bands produced by all the various techniques visualized a fundamental organization of mammalian chromosomes