(The)Relationship between job stress and musculoskeletal symptoms of operating room nurses

학위논문(석사) --서울대학교 대학원 :보건학과(보건학전공),2007.Maste

Repetitive Sequences in DNA of Mouse Tumor Cells

The kinetics of reassociation of the DNA of mouse tumor cells has been examined to determine the amount and repetition frequency of various DNA fractions. Before the reassociation experiment the purity of DNA preparations was confirmed by its melting behavior(temperature denaturation). The Guanine-Cytosine content of DNA was also calculated from the melting curve. (Trn value) The transplantable tumor used in the denaturation experiment were Ehrlich ascites tumor, Sarcoma 180, Yun salivary adenocarcinoma and Yun liposarcoma. Among 4 kinds of mouse tumors, Ehrlich ascites tumor and Yun salivary adenocarcinoma were used in the reassociation experiment. The results were analyzed by comparing the pooled DNA of normal liver cells with that of tumor cells. The following results were obtained; 1. The Guanine-Cytosine contents of each DNA are as follows. Ehrlich ascites tumor 41. 5%(Tm: 86. 3°C) Sarcoma 180 41. O%(Tm: 86. 1°C) Yun salivary adenocarcinoma 39.5%(Tm: 85. 5°C) Yun liposarcoma 40.0%Cfm: 85. 7°C) normal liver cell 41. O%(Tm: 86. 1°C) 2. The amount of non-repetitive DNA of the tumor cells decreased by about 15 percent, compared with that of normal liver cell DNA. 3. There are great differences of amount and repetitive frequency between normal and tumor cell DNA, especially in the intermediate repetitive fraction. The amount of highly repetitive DNAs of tumor cells is increased 2~5% over that of normal cell DNA. In the case of the intermediate fraction, its amount in the tumor cells is approximately twice that of normal cells. In addition to the increase in amount of repetitive sequences, the frequency of repetition is also increased in both fractions of each tumors. The average nucleotide repetition length in the intermediate fraction of the tumor cell DNA is approximately half that of the correspondng length in the normal cell DNA fractio

Y-Fluon:scence III Humall Interphase Nuclei

To evaluate the rate of appearance of Yfluorescent body in interphase nuclei of normal human buccal smears, 50 coded slides (25 males and 25 females) were obtained from the medical student volunteers, stained with either one of the 3 staining solutions: quinacrine mustard buffer;quinacrine dihydrochloride water and quinacrine dihydrochloride buffer. The stained slides were blindly scorered with a count of 100 nuclei. Although four slides were found to be misclassified, generally prediction of the sex of the individual from whom the specimen was taken was satisfactory. The mistake in classification took place mainly because of poor technical quality of the smears, which could be predicted even before decoding process. According to our experience this technique seems to be very useful in sex determination of the interphase nuclei , being complementary to the conventional sex chromatin body study

Banding Patterns of Normal Human Chromosomes

To establish a new technique for chromosome banding, 20 human chromosome preparations (10 males and 10 females) were obtained from the medical school volunteers, and were stained with Giemsa-trypsin-EDTA solution. The stained slides showed banding patterns clearly in each chromosome. The bands produced by trypsin-EDTA treatments appeared to be similar to those found in fluoresecent and other karyotypes; and it was confirmed that the bands produced by all the various techniques visualized a fundamental organization of mammalian chromosomes