MICROBE WITH TRICHLOROPHENOL MAGNETIZATION PROPERTIES AND WASTEWATER TREATMENT METHOD INCLUDING TRICHLOROPHENOL USING THIS MICROBE

본 발명은 2,4,6-트리클로로페놀을 자화시키는 능력을 갖는 신규한 미생물을 제공한다. 공장 폐수로부터 분리된 본 발명의 균주는 슈도모나스 솔라나세아룸(Pseudomonas solanacearum) TCP 114(KCTC 0106BP)으로서 고농도의 2,4,6-트리클로로페놀을 자화시킬수 있고, 이러한 자화능은 포도당 또는 숙신산 같은 분해가 용이한 탄소원이 존재하는 경우에도 저하되지 않는다. 더욱이 아닐린, p-크레졸 또는 벤조산 같은 방향족의 독성 화합물을 분해하는 능력도 나타낸다. 따라서, 본 발명의 균주는 2,4,6-트리클로로페놀을 다량 함유하는 공업용 폐수, 특히 2,4,6-트리클로페놀 뿐만 아니라 방향족 화합물을 함유하는 펄프 또는 제지공장의 페수를 처리하는데 유용하게 이용될 수 있다

분리된 미생물의 순수 및 혼합배양에 의한 염화페놀의 생분해

학위논문(박사) - 한국과학기술원 : 생물과학과, 1996.8, [ ix, 99 p. ]Three strains to degrade chlorophenols were isolated through a selective enrichment procedure with 2,4,6-trichlorophenol (TCP) and 4-chlorophenol (4-CP). On the basis of their morphological and phenotypic characteristics, TCP degrading strain was identified as Pseudomonas solanacearum TCP114, and two 4-CP degrading strains were done as Pseudomonas testosteroni CPW301 and Arthrobacter ureafaciens CPR706, respectively. A 4-CP degrading bacterium, Pseudomonas testosteroni CPW301, dechlorinated and degraded 4-CP via a meta-cleavage pathway. CPW301 could also utilize phenol as a carbon and energy source without the accumulation of any metabolites. Resting cell experiments and enzyme assays of the cell extract indicated that both 4-CP and phenol were degraded via the same meta-cleavage pathway. When phenol was added as a secondary substrate, CPW301 could degrade 4-CP and phenol simultaneously. The addition of phenol enhanced the 4-CP degradation rate greatly because of the increased cell mass and 4-CP degradation activity. Another 4-CP degrading strain, Arthrobacter ureafaciens CPR706, produced hydroquinone as transient intermediate of 4-CP degradation. This result indicated that the chloro-substituent of 4-CP was eliminated to form hydroquinone, which was demonstrated to be oxidized by enzyme extracts from 4-CP grown cells. CPR706 exhibited much higher tolerance for 4-CP than CPW301, as indicated by the maximum degradable concentration (1.6 mM for CPR706 and 0.8 mM for CPW301). The average 4-CP degradation rate by CPR706 was 13 fold higher than that by CPW301 due to the better specific degradation rate and cell yield of CPR706 (0.32 g cell/g 4-CP) than those of CPW 301. CPR706 was demonstrated to degrade other para-substituted phenols through hydroquinone. The degradability of one component by a pure culture was strongly affected by the presence of other compounds in the medium. For example, when all three components (TCP, 4-CP, and phenol) were present in the me...한국과학기술원 : 생물과학과

A MICROORGANISM OF PIMELOBACTER GENUS USEFUL TO ANALYSE PYRIDINE

본 발명은 피멜로박터(Pimelobacter)속에 속하며 피리딘 분해능을 갖는 균주(KCTC 0074BP)를 제공한다. 이 균주는 피리딘 분해능이 우수하며, 폐수중에 존재할 수도 있는 여러 유기물질의 존재하에서도 우수한 피리딘 분해능을 나타내며, 그외 다른 난분해성 물질도 분해할 수 있다. 본 발명은 또한 상기 미생물을 이용하여 피리딘-함유 폐수를 처리하여 피리딘을 제거하는 방법을 제공한다