식품 가공 조건에 따른 십자화과 식물의 글루코시놀레이트 분해산물 연구

학위논문 (석사)-- 서울대학교 대학원 : 식품영양학과, 2012. 2. 권훈정.Glucosinolate(GLS)는 주로 십자화과 식물에 분포하는 2차 대사산물로 식물체 조직에 손상이 가해지면 격리되어 있던 myrosinase의 작용을 받아 분해가 시작된다. Glucosinolate의 분해는 isothiocyanate(ITC), nitrile, thiocyanate와 같은 분해산물들을 생성하는데, 이러한 산물들은 갑상선에 독성효과를 미치는 것으로 알려졌다. 이번 연구에서는 다양한 식품 내 환경을 적용하였을 때 glucosinolate의 분해에 대해 조사하였다. 배추, 무, 유채, 겨자에서 progoitrin, sinigrin, glucoraphasatin, gluconasturtiin, glucobrassicin를 대상으로 산도와 온도, 효소의 활성화에 따라 분해를 관측하였다. 대부분의 군에서 nitrile이 주요 분해산물로 나타났으며, 상대적으로 낮은 pH군과 가열군에서 nitrile의 생성이 더 강하게 주도되었다. 발효의 경우 pH4군과 같은 산도를 지님에도 불구하고, ITC의 생성이 모든 glucosinolate에서 증가하는 방향으로 패턴이 나타났다. 또한 배추에 풍부한 indole계열의 glucosinolate 때문에, 상대적으로 낮은 glucosinolate 농도를 지니고 있음에도 불구하고 SCN-이온이 상당량 검출되었다. 한국에서 많은 십자화과 식물을 발효된 형태인 김치로 섭취하는 것을 감안한다면, 발효의 효과를 고찰할 수 있는 추가적인 분해패턴 연구와 세심한 글루코시놀레이트의 위해성 평가가 필요할 것이다.Maste

Dorfin/Rnf19a E3 ligase 결핍 생쥐의 특성 연구

학위논문(박사) - 한국과학기술원 : 의과학대학원, 2016.2 ,[v, 99 p. :]The ubiquitin-proteasome system is one of the key mechanisms to degrade mis-folded and aggregated proteins. Ubiquitination has been implicated in the regulation of both neuronal development and synaptic plasticity. Dorfin, also known as Rnf19a, is E3 ubiquitin ligase and has been implicated in neurodegenerative diseases such as Amyotrophic lateral sclerosis and Parkinson’s disease. I here report that Dorfin interacts with PSD-95, an important scaffolding protein at excitatory synapses. $Dorfin^{-/-}$ mice show reduced adult neurogenesis, increased amplitude of miniature excitatory postsynaptic currents, and enhanced long-term potentiation in the hippocampal dentate gyrus. Behaviorally, $Dorfin^{-/-}$ mice show impaired contextual fear conditioning, but normal levels of cued fear conditioning, fear extinction, spatial learning and memory, object recognition memory, spatial working memory, and pattern separation. I also identified several proteins in Dorfin-mutant ($Dorfin^{-/-}$) mice that are less ubiquitinated than those in wild-type mice. These results suggest that Dorfin may regulate adult neurogenesis, synaptic plasticity, and contextual fear memory.한국과학기술원 :의과학대학원

Negative pressure pulmonary edema in a patient undergoing open rhinoplasty A case report

Rationale: Negative pressure pulmonary edema (NPPE) is associated with serious postoperative complications. Compact nasal packing is always done after an open rhinoplasty procedure which makes it difficult to achieve positive pressure ventilation via a mask if NPPE arises. Patient concerns: A 21-year-old healthy man got an open rhinoplasty, septal perforation repair, and revisional septal reconstruction. After surgery, he became so agitated that it was difficult to calm him. We decided to remove the endotracheal tube. On arrival at the post-anesthesia care unit, he was cyanotic and his SpO(2) had decreased to about 2%. We attempted positive pressure ventilation using mask bagging; however, it was ineffective due to the nasal packing. Diagnoses: Negative pressure pulmonary edema Interventions: Emergent reintubation was immediately done and Ambu bagging was commenced. A considerable pinkish secretion came out of the tube. A T-piece was applied to him using 15 L/min of oxygen supply. The patient was eventually transferred to the intensive care unit of our hospital. Outcomes: On postoperative day (POD) 1, a decision was made to extubate, and the oxygen supply was shifted to 3L/min using a venturi-mask. On POD 2, a chest posteroanterior radiograph was taken and indicated no active lung lesion. The patient was subsequently discharged without any complications. He had no symptoms on POD 6, 11, and 18 at follow-up visits to our outpatient clinic. Lessons: Anesthesiologists should be alert to the possibility of NPPE and its treatment because of its rapid onset but positive clinical outcome if there is a proper intervention. In nasal surgery cases in particular, early re-intubation should be conducted and extubation should be done to fully awaken the patients

Effects of Curcumin Treatment in a Diabetic Neuropathic Pain Model of Rats: Involvement of c-Jun N-Terminal Kinase Located in the Astrocytes and Neurons of the Dorsal Root Ganglion

Curcumin (diferuloylmethane) is a major component of turmeric, which is isolated from the rhizomes of Curcuma longa L. from the family Zingiberaceae. It is used as a dietary pigment for curry and in traditional Indian medicine for its anti-inflammatory and attenuating pain effects. This study aimed to evaluate the beneficial effects of curcumin in a rat model of diabetic neuropathic pain. Additionally, we investigated the involvement of the phosphorylated form of c-Jun N-terminal kinase (pJNK) located in the neurons and astrocytes of the dorsal root ganglion (DRG). To induce diabetic neuropathic pain in rats, 50 mg/kg of streptozotocin (STZ) was intraperitoneally injected. After 4 weeks, rats were administered the vehicle, 10 mg/kg/day curcumin, or 50 mg/kg/day curcumin orally for 4 consecutive weeks. One day after the final drug administration, we performed behavioral tests to measure responses of rats to mechanical, heat, cold, and acetone-induced cold stimuli. After behavioral tests, pJNK expression in the DRG was evaluated using western blot assay and immunohistochemistry. Curcumin treatment for 4 consecutive weeks in STZ-induced diabetic neuropathic pain rats improved behavioral responses to mechanical, cold, and thermal stimuli. Increased pJNK expression in the astrocytes and neurons of the DRG in STZ-induced diabetic neuropathic pain rats was reduced by curcumin treatment for 4 consecutive weeks. We suggest that curcumin can be an option for the treatment of diabetes-related neuropathic pain, and one of the mechanisms that underlie the action of curcumin may involve pJNK expression in the astrocytes and neurons of the DRG

An Orthogonal Polymer Semiconductor Gel for Solution Tandem Electronics

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