Battery-free Lab-on-a-chip for Portable Immunosensors

학위논문(박사)--아주대학교 일반대학원 :전자공학과,2009. 2ABSTRACT i LIST OF FIGURES ii LIST OF TABLES iii LIST OF PARAMETERS iii Chapter I. INTRODUCTION 1 1.1 immunosensing lab-on-a-chip 1 1.2 Review of microfluidics of immunosensing LOC 4 1.3 Review of on-chip power mechanism 6 1.4 Objectives of the dissertation 9 Chapter II. PRELIMINARY THEORIES 10 2.1 Reynolds number 10 2.2 Surface tension and capillary effect 12 2.2.1 Surface tension in micro-environment 12 2.2.2 Capillary effect 13 2.2.3 Capillary stop valve 15 2.3 Fluidic resistance in the rectangular microchannel 19 2.4 Detection method proper to the portable immunoassay 20 2.4.1 The components of immunosensor 20 2.2.2 “Back filling” immunoassay 21 Chapter III. Immunosening LOC Controlled by Surface Tension 24 3.1 Design 24 3.1.1 Structure 26 3.1.2 Immune-electrode design 28 3.2 Fabrication 30 3.3 Results and discussions 32 3.3.1 Flow characterization 32 3.3.2 Electrochemical immunoassay 35 Chapter IV. One-Touch Type LOC with PDMS Check Valves 41 4.1 Design 41 4.1.1 Structure 42 4.1.2 Operation principle 45 4.2 Fabrication 46 4.3 Results and discussions 50 4.3.1 Flow characterization 50 4.3.2 Electrochemical immunoassay 54 Chapter V. A Multi-stacked Hand-Operated LOC with Single Latch 60 5.1 Design 60 5.1.1 Structure 60 5.1.2 Operation principle 61 5.2 Fabrication 67 5.2.1 Fabrication of functionalized immunosensors 67 5.2.2 Fabrication of the immunosensing LOC 68 5.3 Results and discussions 73 5.3.1 Flow characterization 73 5.3.2 Electrochemical immunoassay 83 Chapter VI. A Sophisticated LOC integrated with Latches 89 6.1 Design 90 6.1.1 Structure & operation principle 90 6.1.2 Multi-channel Flow characteristics 94 6.1.3 Immunosensing strategy 97 6.2 Fabrication 99 6.3 Results and discussions 104 6.3.1 Flow characterization 104 6.3.2 Back-filling immunoassay 115 Chapter VI. Conclusions and Outlook 118 REFERENCES 121 PUBLICATION LIST 134Maste