바벨 로우(Row) 운동 시 리프팅 스트랩의 사용이 운동역학적 변인에 미치는 영향

학위논문 (석사)-- 서울대학교 대학원 : 체육교육과, 2015. 8. 신인식.본 연구의 목적은 리프팅 스트랩 사용이 벤트 오버 바벨 로우(bent-over barbell row) 동작 및 관련근육 활성화에 미치는 영향을 확인하고 그 유용성을 검토하는데 있다. 10명의 실험 참가자는 시, 도 단위 보디빌딩 대회 참가경력이 있는 성인 남자선수들로써, 교차설계(crossover)를 통해 모든 연구 대상자는 6-RM의 중량으로 두 가지 조건(W: With strap/WO: Without strap)에서 2세트(12회), 총 4세트(24회)의 바벨 로우를 수행하였다. 세트 간 휴식시간은 1분 30초, 조건 간 휴식시간(wash-out period)는 15분을 갖도록 했다. 그 결과, 리프팅 스트랩 사용은 바벨 로우 동작 시 수행시간, 바(bar)의 평균속도, 무릎 최대 각속도 및 관절 가동범위에 영향을 주지 않는 것으로 나타났다. 하지만, 후기 원심성수축구간(LPEC)에서 W조건이 상완이두근 근활성도가 유의하게 낮게 나타났다(2세트 전-후반부 비교, 1세트 전-2세트 후반부 비교). 실험을 통하여 얻은 결론은 다음과 같다. 벤트 오버 바벨 로우 동작 시 리프팅 스트랩의 사용은 외관상으로는 뚜렷한 영향을 미치지 않지만, 후기 원심성수축구간(LPEC)에서 상완 이두근의 역할을 보조하는 것으로 보인다. 한 부위에 대하여 여러 운동을 다중 세트로 실시하는 보디빌더의 훈련프로그램에서 이러한 효과는 상완 이두근의 피로를 지연시킴으로써, 목표근육을 훈련할 수 있는 시간을 연장시키는데 도움이 될 것으로 기대된다.목 차 Ⅰ. 서 론 1 1. 연구의 목적 3 2. 연구의 가설 4 3. 연구의 제한점 4 4. 용어의 정의 5 Ⅱ. 이론적 배경 7 1. 벤트 오버 바벨 로우(Bent-over barbell row) 7 2. 웨이트트레이닝과 보조 장비 8 3. 리프팅 스트랩(Lifting strap)의 효과 12 4. 근 피로와 운동수행능력 13 5. 웨이트트레이닝과 휴식시간 16 Ⅲ. 연구 방법 17 1. 연구 대상 17 2. 실험도구 18 1) 측정 장비 18 2) 바벨 로우에 사용된 리프팅 스트랩 19 3. 실험 절차 20 4. 자료분석 24 1) 이벤트와 구간의 설정 24 2) 동작 분석 26 3) 근전도 분석 27 5. 통계처리 28 Ⅳ. 연구 결과 29 1. 리프팅 스트랩 사용 유/무에 따른 동작 수행시간 29 2. 리프팅 스트랩 사용 유/무에 따른 바(Bar)의 궤적 및 평균속도 33 1) 바(Bar)의 궤적 33 2) 바(Bar)의 평균속도 40 3. 리프팅 스트랩 사용 유/무에 따른 근활성도 43 1) 1세트 전반부와 후반부 43 2) 2세트 전반부와 후반부 48 3) 1세트 전반부와 2세트 후반부 53 4. 리프팅 스트랩 사용 유/무에 따른 무릎 최대 각속도 및 관절가동범위 62 1) 무릎 최대각속도 62 2) 무릎 관절 가동범위 64 Ⅴ. 논의 66 1. 리프팅 스트랩 사용 유/무에 따른 동작 수행시간 66 2. 리프팅 스트랩 사용 유/무에 따른 바(Bar)의 궤적 및 평균속도 67 3. 리프팅 스트랩 사용 유/무에 따른 근활성도 70 4. 리프팅 스트랩 사용 유/무에 따른 무릎 최대 각속도 및 가동범위 72 Ⅶ. 결론 73 참 고 문 헌 77 Abstract 85Maste

In vitro study on Arthrospira (Spirulina) maxima extract as a fetal bovine serum-substituted HeLa cell culture medium

The HeLa cell lines are widely important for the research area of cell biology, cancer, virus propagation, biosynthesis and anti-tumor mechanism research as a tumor model. For culturing these cells, fetal bovine serum (FBS) is generally used as a main serum supplement. However, there are several problems with the use of FBS such as high cost, unclear components, and unstable supply. In addition, ethical concerns were raised during the upstream process for manufacturing FBS though the serum is important in the field of worldwide cell industry. For this reason, searching for serum alternatives and development of serum-free media has been attracting global attention. In this study, the possibility of cell culture medium as FBS substitute using Spirulina maxima extract (SE) was validated. To evaluate the possibility of FBS alternatives using SE, we manufactured different serums of 4 groups such as [10% FBS], [5% FBS/5% SE], [3% FBS/7% SE], and [1% FBS/9% SE] according to the medium ratio of FBS to SE in HeLa cell line. Cell viability, morphology, protein expression pattern, cell cycle analysis, and apoptotic analysis were analyzed for identifying cell conditions affecting by concentrations of SE-based substitute medium. In the results, two groups were not showed significantly differences compared with control group of [10% FBS] in HeLa cell lines without the group of [1% FBS/9% SE]. From the results of this study, S. maxima extract could be an affordable solution for the FBS-alternated growth medium of the HeLa cell lines.2

PRODUCTION METHOD OF PECTIN USING MICROALGAE

본 발명은 미세조류 재료를 준비하는 단계 상기 미세조류 재료를 용매와 혼합하는 단계 및 상기 혼합물 을 열 추출하는 단계를 포함하는 펙틴의 추출, 제조 방법에 대한 것이다

GLYCOGEN HYDROLYSIS OF CYANOBACTERIA BY FUNGAL AMYLOLYTIC ENZYME COMPLEX FOR BIOETHANOL PRODUCTION

Photosynthetic microorganisms have garnered increased interest as the biomass of choice for renewable energy production. These organisms, namely microalgae and cyanobacteria, have advantageous characteristics for biofuel production when compared to conventional agricultural crops. First and foremost, the use of microorganism biomass for fuel production does not negatively impact food and feed. Also, these microorganisms have much higher net energy balance (NEB) and require significantly less arable land, if any, for more efficient mass cultivation than agricultural crops. Cyanobacteria primarily store glycogen as the carbohydrate reserve, with certain strains reaching upto 50% glycogen as its biomass under favorable growth conditions. For effective saccharifcation of cyanobacteria, developments of high-yield and low cost amylases are needed. In this study, we isolated a new Trichoderma species J113 strain from the coastal terrains of Korea and determined that the fungus has a high amylolytic enzyme activity. We cultured the fungus on wheat bran to stimulate enzyme production, and the crude extract was subsequently purified through filtrations, precipitation, and chromatography. We learned that J113 enzyme complex consists of two major amylases, Ayt40 and Ayt70, that were determined as an endo-amylase and an exo-amylase, respectively. While these two amylases exhibited different pH and temperature requirements for optpared to conventional agricultural crops. First and foremost, the use of microorganism biomass for fuel production does not negatively impact food and feed. Also, these microorganisms have much higher net energy balance (NEB) and require significantly less arable land, if any, for more efficient mass cultivation than agricultural crops. Cyanobacteria primarily store glycogen as the carbohydrate reserve, with certain strains reaching upto 50% glycogen as its biomass under favorable growth conditions. For effective saccharifcation of cyanobacteria, developments of high-yield and low cost amylases are needed. In this study, we isolated a new Trichoderma species J113 strain from the coastal terrains of Korea and determined that the fungus has a high amylolytic enzyme activity. We cultured the fungus on wheat bran to stimulate enzyme production, and the crude extract was subsequently purified through filtrations, precipitation, and chromatography. We learned that J113 enzyme complex consists of two major amylases, Ayt40 and Ayt70, that were determined as an endo-amylase and an exo-amylase, respectively. While these two amylases exhibited different pH and temperature requirements for opt1

Method for aggregating microalgae using strongly alkaline electrolyzed water and aggregates thereof

본 발명에 의한 강알칼리 전해수를 이용한 미세조류 응집방법은, (a) 미세조류를 포함하는 배양배지를 준 비하는 단계 (b) 상기 배양배지에 강알칼리 전해수를 투입하여 미세조류 응집체를 형성하는 단계 및 (c) 상기 미세조류 응집체와 여액을 분리하여 상기 미세조류 응집체를 획득하는 단계 를 포함하는 것을 특 징으로 한다

Extraction and Characterization of Pectin-like Polysaccharide from Arthrospira (Spirulina) maxima

Pectin or pectin-like polysaccharide (PLP) are one of the most complex polysaccharide in plant cell walls, and it’s consist on linked galacturonic acid polymer as a backbone and various sugars in side chain. The functions of PLP are the substrates of gelling and stabilizing polymers in food industry. Furthermore, PLP has an important role in medicinal removal of heavy metals and anticancer activity reported to be beneficial for human health. The aim of this study was to extract, characterize and compare the PLPs isolated from Arthrospira (Spirulina) maxima and citrus peel as a control sample with different chemicals and methodologies. The yields of the extracted PLP isolated from A. maxima were 21.06±3.5% in distilled water (DW) and 24.48±1.2% in acidic condition (AC), whereas the yields of PLP from citrus peel of 8.68±1.2% (DW) and 9.37±0.6% (AC), respectively. The yields of galacturonic acid of PLP from A. maxima were 8.40±0.1% (DW) and 8.36±0.1% (AC). We will be discussed in progress and further studiesbstrates of gelling and stabilizing polymers in food industry. Furthermore, PLP has an important role in medicinal removal of heavy metals and anticancer activity reported to be beneficial for human health. The aim of this study was to extract, characterize and compare the PLPs isolated from Arthrospira (Spirulina) maxima and citrus peel as a control sample with different chemicals and methodologies. The yields of the extracted PLP isolated from A. maxima were 21.06±3.5% in distilled water (DW) and 24.48±1.2% in acidic condition (AC), whereas the yields of PLP from citrus peel of 8.68±1.2% (DW) and 9.37±0.6% (AC), respectively. The yields of galacturonic acid of PLP from A. maxima were 8.40±0.1% (DW) and 8.36±0.1% (AC). We will be discussed in progress and further studies1

Optimization of up- and down-stream processes for microalga-based mammalian cells culture media

FBS is the most widely used serum-supplement for in vitro cell culture of animal cell lines. However, sustainability of FBS industry has aware of ethical, economics and environmental issues despite the serum is important in the field of worldwide cell industry. The development of the serum-substituted media is being remarkable with strong interest in worldwide. In this study, we designed for optimization processes from upstream to downstream in order to make a FBS-alternative solution using in situ cultured Spirulina maxima biomass. S. maxima were cultured quarterly using by ROSE-max culture system, and were used as a raw material for manufacturing Spirulina Animal Cell Culture Solution (denoted as SACCS). Thepreprocessing method of SACCS was established by the processes via S. maxima cell milking, extraction and filters system. In the results, quality of the serial SACCS production based on Lot# was confirmed by contamination of mycoplasma, fungi and bacteria by PCR, and then all of the samples were not detected. Endotoxin test showed safe level below 5 EU/ml according to the guideline of ISIA (the International Serum Industry Association). Protein, carbohydrate, fat and minerals were analyzed and presented. Heavy metals were insignificantly detected by the inorganic analysis. For identifying adaptable cells culture, T24 (human urinary bladder cancer model) and H358 (lung cancer tumor model) cells were then applied2

Chitosanase signal peptide from Bacillus subtilis its lead target protein to periplasmic space in Escherichia coli

We isolated chitosanase secreting B. subtilis CH2 and identified the chitosanase sequence. Analyzed the sequence showed that it consisted of 813 bp, including 87 bp signal sequence. The signal sequence leads the target protein to the cell-membrane of the B. subtilis CH2 and then secret the chitosanase out of the cell. The signal peptide showed 6 amino acid deletion compared to other B. subtilis chitosnase signal peptides. The chitosanase sequence including signal peptide was cloned into pET11a vector without fusion and expressed in E. coli BL21(DE3). The expressed chitosanase in E. coli showed two distinct bands which represent the pro-chitosanase in cytoplasm and mature chitosanase in periplasm. Time frame induction and results showed that muture chitosanase was increased. Subsequently, we linked this chitosanase signal sequence in front of B. subtilis CH2 mature lichenase and xylanase sequences, and expressed it in E. coli BL21(DE3). The recombinant xylanase moved to periplasmic space. However, the recombinant lichenase did not move to periplasm.embrane of the B. subtilis CH2 and then secret the chitosanase out of the cell. The signal peptide showed 6 amino acid deletion compared to other B. subtilis chitosnase signal peptides. The chitosanase sequence including signal peptide was cloned into pET11a vector without fusion and expressed in E. coli BL21(DE3). The expressed chitosanase in E. coli showed two distinct bands which represent the pro-chitosanase in cytoplasm and mature chitosanase in periplasm. Time frame induction and results showed that muture chitosanase was increased. Subsequently, we linked this chitosanase signal sequence in front of B. subtilis CH2 mature lichenase and xylanase sequences, and expressed it in E. coli BL21(DE3). The recombinant xylanase moved to periplasmic space. However, the recombinant lichenase did not move to periplasm.1

해양 유래 펙틴 원천소재의 분리,정제 및 응용기술 개발

제1장 서론 1절. 연구의 필요성 2절. 연구의 목표 제2장 국내외 기술개발 현황 1절. 기술·경제･사회적 측면 2절. 정책 동향 분석 3절. 국외 기술개발 현황 4절. 국내 기술개발 현황 5절. 펙틴 특허동향 제3장 연구개발 수행 내용 및 결과 1절. 해양조류 유래 펙틴 라이브러리 구축 2절. 해양 유래 펙틴의 이화학적 특성 분석 및 정제기술 개발 3절. 펙틴 특성분석 및 독성테스트 4절. 펙틴 나노입자 합성 및 분석 5절. 펙틴 융합 의료용 멤브레인 겔 제작, 특성 및 효능평가 제4장 연구개발 목표 달성도 및 대외기여도 1절. 대표적 성과(정성적 달성도) 제5장 연구개발 결과의 활용계획 1절. 기대효과 2절. 연구개발결과의 활용방안 제6장 참고문헌한국해양과학기술

해양 유래 펙틴 원천소재의 분리,정제 및 응용기술 개발

제1장 서론 1절. 연구의 필요성 2절. 연구의 목표 제2장 국내외 기술개발 현황 1절. 기술·경제･사회적 측면 2절. 정책 동향 분석 3절. 국외 기술개발 현황 4절. 국내 기술개발 현황 5절. 펙틴 특허동향 제3장 연구개발 수행 내용 및 결과 1절. 해양조류 유래 펙틴 라이브러리 구축 2절. 해양 유래 펙틴의 이화학적 특성 분석 및 정제기술 개발 3절. 펙틴 특성분석 및 독성테스트 4절. 펙틴 나노입자 합성 및 분석 5절. 펙틴 융합 의료용 멤브레인 겔 제작, 특성 및 효능평가 제4장 연구개발 목표 달성도 및 대외기여도 1절. 대표적 성과(정성적 달성도) 제5장 연구개발 결과의 활용계획 1절. 기대효과 2절. 연구개발결과의 활용방안 제6장 참고문헌한국해양과학기술