에너지·산업부문 온실가스 통합정보 관리시스템 구축

funder : 지식경제부agency : 지식경제부제 1장 서론 1.연구의 필요성 정부의 저탄소녹색성장기본법에 따른 녹색성장 정책은 에너지 및 온실가스 통계의 체계적인 관리의 필요성을 증대시키고 있다.이는 에너지 효율향상 및 기후변화에 필요한 정책 개발 및 정책 이행에 따른 모니터링에 있어 필요로 하는 기초자료가 되기 때문이다. 국가 정책과 최종 에너지사용자 및 온실가스 배출자간의 상호 연계를 위해서는 에너지 사용량 및 온실가스 배출량,에너지 기술에 따른 잠재량,감축옵션 등의 연계된 데이터가 필요로 하지만,현재 우리나라에서는 각 기관별 관리로 인해 자료 확보가 용이하지 않은 현실이다

Preparation of Chitin/Chitosan Nanoparticles by Electrospray

최근 의약학용 바이오 소재로 각광을 받고 있는 키틴과 키토산을 전기적인 힘을 이용하는 전기방사장치를 사용하여 미세입자 형태로 제조하였다. 키틴 또는 키토산 시료에 대하여 고분자 농도가 일정한 수준(사슬 간의 엉킴이 본격적 일어나는 농도) 이상에서는 초극세 섬유의 형태를 보였으며, 그 농도 이하에서는 비교적 균일하고 미세한 입자를 형성하였다. 키토산의 경우, 5% 초산 수용액에 녹여 4~5 wt%로 분사하였을 경우 200~400nm 크기의 미세입자를 제조할 수 있었다. 또한 농도가 증가할수록 입자의 크기가 커지면서 다소 불균일해지는 성향을 보였다. 수용성 글리콜 키토산은 분자량이 적기 때문에 높은 농도인 약 55 wt% 까지 미세입자가 생성되었다. 이 입자의 평균크기는 약 300­500 nm 정도를 나타내었다. 키토산을 초산 수용액에 녹여서 무수 아세트산으로 처리하여 얻어진 N­아세틸화 키틴의 경우에는 포름산에 농도별로 녹여 전기분사를 하였는데 7 wt% 이하에서 균일한 미세입자가 생성되었다. 따라서, 키틴이나 키토산의 미세입자를 제조하기 위해서는 용매에 따라 일정한 농도와 점도와의 관계곡선으로부터 사슬간의 엉킴현상에 의해 섬유상이 형성되지 않은 낮은 농도에서 전기분사를 실시하는 것이 무엇보다도 중요하다는 것을 알 수 있었다.과학기술부 나노핵심기반기술사

Relation of the radiologic findings and labeling index of Ki-67, PCNA and cytokeratin in unicystic ameloblastoma, dentigerous cyst and odontogenic keratocyst

Purpose: To compare the proliferation potential of the epithelial cells between unicystic ameloblastoma (UA), dentigerous cyst (DC), and odontogenic keratocyst (OKC) and to correlate this proliferation potential with the radiographic features of these three pathoses. Materials and Methods: Immunohistochemical expression of PCNA, Ki-67, and cytokeratin as a proliferation marker were assessed for 15 cases of UA, 15 cases of DC, and 15 cases of OKC. The degree of immunochemical expression of three proliferation markers were correlated with the radiographic features, especially cortical expansion (negative and positive) and shape of border (scalloped and round). Results: Using PCNA and Ki-67, OKC showed the highest proliferation potential and UA the lowest. Statistically significant differences were found between the OKC and the UA (p < 0.05). However, no statistically significant difference was present according to the radiographic features in all pathoses. Using cytokeratin, there was no significant differences of proliferation potential among three pathoses. Conclusions : OKC epithelium has the most intense proliferation potential, followed by the dentigeous cyst and then unicystic ameloblastoma. There is no significant relation between the radiographic features and the proliferation potential of epithelium of these three pathoses.이 논문은 2000년도 서울대학교 병원(04-2000-059)연구비 지원에 의해 이루어 진 것임

A laminin-derived functional peptide, PPFEGCIWN, promotes bone formation on sandblasted, large-grit, acid-etched titanium implant surfaces

Purpose: This study aimed to investigate the in vitro and in vivo bone-forming potential of a sandblasted, large-grit, acid-etched (SLA) titanium (Ti) surface treated with a laminin-derived functional peptide, PPFEGCIWN (DN3). Materials and Methods: Human osteoblast-like MG63 cells were cultured with SLA Ti disks that were untreated or treated with DN3 or a control scrambled peptide. Cell adhesion, spreading, and viability on the disks were tested. Alkaline phosphatase gene expression and enzyme activity were also evaluated. Four DN3-coated SLA Ti implants and four untreated implants were placed into the tibiae of two rabbits (two implants/tibia). Ten days later, the bone-implant interfaces were subjected to histomorphometry to measure the bone response. The surface properties of the disks and implants were determined using scanning electron, widefield confocal, and confocal laser microscopy and x-ray photoelectron spectroscopy. Results: The peptide-treated and untreated disks and implants were similar in terms of physical surface properties, but the peptide-treated surfaces had significantly higher nitrogen levels (P &lt; .05). The DN3 peptide promoted cell adhesion, spreading, and alkaline phosphatase expression and enzyme activity (P &lt; .05). Histomorphometry of the harvested implants showed rapid bone formation and affinity of the motif. Conclusion: This study suggests that treatment with the cell adhesion peptide DN3 promotes bone healing at the SLA Ti surface.OAIID:RECH_ACHV_DSTSH_NO:T201909987RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A001644CITE_RATE:1.734DEPT_NM:치의과학과EMAIL:[email protected]_YN:YY

A Vitronectin-derived bioactive peptide improves bone healing capacity of SLA titanium surfaces

In this study, we evaluated early bone responses to a vitronectin-derived, minimal core bioactive peptide, RVYFFKGKQYWE motif (VnP-16), both in vitro and in vivo, when the peptide was treated on sandblasted, large-grit, acid-etched (SLA) titanium surfaces. Four surface types of titanium discs and of titanium screw-shaped implants were prepared: control, SLA, scrambled peptide-treated, and VnP-16-treated surfaces. Cellular responses, such as attachment, spreading, migration, and viability of human osteoblast-like HOS and MG63 cells were evaluated in vitro on the titanium discs. Using the rabbit tibia model with the split plot design, the implants were inserted into the tibiae of four New Zealand white rabbits. After two weeks of implant insertion, the rabbits were sacrificed, the undecalcified specimens were prepared for light microscopy, and the histomorphometric data were measured. Analysis of variance tests were used for the quantitative evaluations in this study. VnP-16 was non-cytotoxic and promoted attachment and spreading of the human osteoblast-like cells. The VnP-16-treated SLA implants showed no antigenic activities at the interfaces between the bones and the implants and indicated excellent bone-to-implant contact ratios, the means of which were significantly higher than those in the SP-treated implants. VnP-16 reinforces the osteogenic potential of the SLA titanium dental implant.OAIID:RECH_ACHV_DSTSH_NO:T201913733RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A001644CITE_RATE:2.467DEPT_NM:치의과학과EMAIL:[email protected]_YN:YY

MicroRNA-146a-5p Limits Elevated TGF-β Signal during Cell Senescence

OAIID:RECH_ACHV_DSTSH_NO:T201703162RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A001644CITE_RATE:6.392DEPT_NM:치의과학과EMAIL:[email protected]_YN:YY