Smart evacuation analysis platform based on IMO MSC.1/Circ.1533

IMO(International Maritime Organization)에서는 국제 해사법으로써 여객선의 설계시 비상시를 대비한 대피 및 탈출 규정을 명시하고 있다. 2016년 새롭게 발효된 \\IMO MSC.1/Circ.1533에는 새롭게 2020년 1월부터 신건조되는 여객선 부터는 반드시 Evacuation Analysis를 수행해야 한다. 그러나 IMO MSC.1/Circ.1533의 기준을 모두 반영하여 Evacuation analysis를 수행할 수 있는 플랫폼은 전세계적으로 전무한 실정이다. 여객선 대피 탈출의 경우 Evacuation analysis를 수행하는데 큰 어려움이 있는데, 선박의 침몰시 배가 기우는 현상, 위험물의 폭발 및 가스 누출, 낮과 밤의 승객의 행동 양상의 상이함 등으로 인해 신뢰성 높은 Evacuation analysis를 수행하는데 어려움이 있다. 본 학위논문에서는 선박의 사고 상황을 분류하여 침몰, 폭발 및 가스 누출, 그리고 IMO MSC.1/Circ.1533에서 규정한 낮과 밤의 승객의 행동양상의 특성을 모두 고려가능한 Smart Evacuation Analysis Platform을 제안한다. 추가적으로 위험을 인지하여 탈출 경로를 바꾸는 인지 반응 알고리즘과 인지 반응 시간을 고려한 알고리즘을 제안하였다.한국과학기술원 :기계공학과,학위논문(박사) - 한국과학기술원 : 기계공학과, 2021.8,[vi, 102 p. :

Localization of Carbonic Anhydrase(Cytoplasm and Periplasm) Orignated from Synechocystis PCC6803 and E.coli(K12)

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생물촉매를 이용한 이산화탄소의 탄산칼슘 전환 연구

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Chaperone co-expression with organophosphorous hydrolase following twin arginine translocation pathway in E. coli and its enhanced whole cell activity

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Conversion of Carbon Dioxide to Calcium Carbonate using Recombinant Biocatalyst

MasterSeveral carbon dioxide (CO2) capture and storage (CCS) technologies have been developed for reduction of CO2 which is the major anthropogenic green house gas. As a new paradigm, there have been attempts to convert CO2 to valuable chemicals. Recently, as a mimic of natural biomineralization, use of carbonic anhydrase (CA) which is an enzyme catalyzing reversible hydration of CO2 to bicarbonate has been suggested to biologically convert CO2 to carbonate minerals. Purified bovine CA (BCA) has been commonly utilized in the previous studies. However, due to economical problem of BCA preparation, practical utilization on biological CO2 conversion has been limited. Here, we focused on biocatalytic capture by recombinant Neisseria gonorrhoeae carbonic anhydrase (NCA), followed by trapping as solid CaCO3 using whole cell biocatalyst.In the present work, we investigated conversion of CO2 to calcium carbonate (CaCO3) as a target model carbonate minerals by using economical recombinant whole cell biocatalyst. To our knowledge, this is the first report on usage of recombinant CA for biological CO2 conversion. Recombinant α-type CA originated from N. gonorrhoeae was highly expressed as a soluble form in Escherichia coli. We found that purified NCA (~99% purity) has comparable hydration activity to the commercial purified BCA. Furthermore, precipitation of CaCO3 was promoted by addition of calcium ion in the presence of NCA and the rate of forming crystal morphology (calcite) was also accelerated. Importantly, whole cell fraction without high cost purification steps also showed comparable conversion ability to the purified NCA and BCA. Collectively, economical recombinant CA in the form of whole cell biocatalyst can be practically applied for conversion of CO2 to high valuable carbonate minerals

Distribution Effect of Cationic Amino Acids on Activity of Antimicrobial Peptides

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