Self-assembled hyaluronic acid nanoparticle as a topical agent for psoriasis treatment

학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2021. 2Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS and METHODS 4 1. Materials 4 2. Preparation and characterization of HA-LCA NP 5 3. Cell culture and treatment 6 4. Mice and induction of psoriasis mouse model 7 5. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) analysis 7 6. Cells viability test 8 7. Cell isolation 9 8. FACS analysis 9 9. Western blot 10 10. Franz diffusion cell (FDC) 11 11. Histological analysis 12 12. Statistical analysis 13 Ⅲ. RESULTS 16 1. Synthetic scheme and characteristics of self-assembled HA-LCA NP 16 2. HA-LCA NP inhibits the polarization of resting macrophages into M1 phenotype 20 3. Effects of free HA and LCA on the expression of M1 marker genes in THP-1 cells 26 4. HA-LCA NP inhibits LPS-induced TLR4 signaling pathway in macrophages 31 5. Effects of HA-LCA NP on the expression of psoriasis-related genes in keratinocytes 34 6. Skin-penetrating ability of HA-LCA NP 37 7. Topical treatment of HA-LCA NP alleviates psoriatic symptoms in IMQ-induced psoriasis mouse model 45 8. Topical treatment of HA-LCA NP inhibits keratinocyte hyperproliferation 50 9. Topical treatment of HA-LCA NP decreases infiltration of macrophages into the dermis 53 Ⅳ. DISCUSSION 56 Ⅴ. REFERENCE 60 Ⅵ. ABSTRACT IN KOREAN (국문초록) 65MasterSelf-assembled hyaluronic acid nanoparticles (HA-NPs) are widely used as drug delivery systems because HA has biocompatible, non-toxic, and non-immunogenic. Recently, drug-free HA-NPs have been shown to exert therapeutic effects in type 2 diabetes and atherosclerosis. Here, I investigate the in vitro and in vivo therapeutic efficacy of an empty HA-NP itself in psoriasis, the most common chronic inflammatory skin disease, after topical treatment via transcutaneous delivery. HA-lithocholic acid (LCA) NPs are amphiphilic compounds composed of hydrophilic HA and hydrophobic LCA that self-assemble in an aqueous environment to form sphere-shaped NPs. HA-LCA NPs inhibited the lipopolysaccharide (LPS)-induced polarization of the human monocyte cell line THP-1 into pro-inflammatory M1 macrophages and reduced the expression of interleukin (IL)-23, a key player in psoriasis and inflammatory cascades. In addition, HA-LCA NPs decreased the expression of pro-inflammatory genes in the human keratinocyte cell line HaCaT stimulated with IL-17 and IL-22. Furthermore, transcutaneous administration of HA-LCA NPs resulted in significant localization into the dermis and epidermis in control and imiquimod (IMQ)-induced psoriasis mouse models, thereby effectively ameliorating psoriasis-like skin inflammation as reflected by reduced skin tissue thickening, inflammation, and cytokine expression. These results suggest that skin-penetrating HA-LCA NPs may serve as novel therapeutic agents for the treatment of human psoriasis-like dermatitis by suppressing the inflammatory response

Factors Associated with Intention for Additional Vaccination in Mothers with Children aged 4~6 Years

Purpose: The study was done to investigate factors in mothers related to their intention to get additional vaccination for their children aged 4~6 years. Methods: A descriptive correlational study was conducted. The participants were 858 mothers conveniently selected from a public health center in Seoul. Data were collected using self-administered questionnaires. Descriptive statistics, t-test, ANOVA, correlation and stepwise multiple regression were used for data analysis. Results: The mothers got a high of 2.08 marks on intention to get additional vaccination. The intention was positively correlated to attitude, perceived benefits, self-efficacy and negatively to perceived barriers, parenting stress. The most significant variables was attitude, perceived benefits, self-efficacy and those three variables explained 42.7% of mother's intention for additional vaccination. Conclusion: The above mentioned findings were identified as significant factors affecting mother's intention for additional vaccination. The findings are expected to make a positive contribution to creating an ideal intervention and to promoting an increased vaccination rate

Quality of Life and Related Factors in Patients with Chronic Hepatitis B

Purpose: This study aimed to investigate LDQOL (Liver Disease Quality of Life) and its related factors of patients with Hepatitis B. Method: A cross-sectional descriptive study. The LDQOL was formally translated to Korean and reliability was examined. One hundred thirty eight patients following gastroenterology outpatient clinic of S. hospital in Seoul, Korea participated. Results: The mean age of the patients was 45.43 years and 87.7% were men. The mean score of LDQOL was 77.34, and mean scores of subscale were followed; symptom of liver disease (SxLD) (82.12), effect of liver disease (ELD) (25.50), concentration (Conc) (84.47), memory (Mem) (83.24), health discomfort, (HD) (75.18), sexual function (SFun) (75.71), sexual problem (SProb) (84.70), Loneliness (85.50), Hopeless (67.43), and stigma of liver disease (SLD) (91.64). Women had a lower LDQOL score for Loneliness (p=.034), and over 45 year-old patients had a lower LDQOL overall score (p=.000). Patients who were HBV carriers, or who had Chronic Hepatitis B or Liver Cirrhosis reported lower QOL respectively(p=.032). Conclusion: Although the liver disease itself seemed to be stable, patients with HBV experienced poor QOL in ELD, SProb, SFun, and Hopeless. Therefore nursing interventions in these aspects are needed

Depression and Quality of Life among Family Caregivers of Patients with Parkinson's Disease in South Korea

ression and quality of life of family caregivers of patients with Parkinson's disease(PD). Methods: A cross-sectional descriptive study was conducted in one neurology outpatient clinic in Seoul, Korea from March to June, 2006. Sixty eight family caregivers of PD patients were participated to the study, using CES-D and SF-36. Results: Mean scores of depression were 16.18±8.39 (range: 0-48) and it was a little lower than caregiver's who took care of Dementia patients and were higher than primary caregivers of the patients with Stroke. Time for caregiving, perceived severity, duration of PD were significantly related with depression respectively. Higher ADL scores which mean greater motor disabilities were related to higher caregiver depression. Lower income and greater medical expenditure were closely related with the depression of family caregivers respectively. The mean scores of total QOL were 435.5±96.5 and the mean scores of PF, SF, RE and MH were lower than general population. Time for caregiving, depression, patients' ADL scores were significantly associated with QOL respectively. People who were older and had lower educational background showed lower QOL scores respectively. Conclusion: Healthcare professionals should pay more attention to emotional aspects of caregivers who take care of PD patients, and develop comprehensive management strategies both for patients and their caregivers