11 research outputs found
λ€μν μ체 κΈ°λ₯κ³Ό κΈ°κ³μ μμ μ±μ κ°λ νμ΄λλ‘μμννμ΄νΈμ μ‘°μ§ μ¬μμ© ν¨ν°λ
νμλ
Όλ¬Έ (λ°μ¬) -- μμΈλνκ΅ λνμ : 곡과λν μ¬λ£κ³΅νλΆ(νμ΄λΈλ¦¬λ μ¬λ£), 2021. 2. κΉνμ΄.μΈμ²΄ λ΄λΆ λΌμ ꡬμ±μμ μ€ νλμΈ νμ΄λλ‘μμννμ΄νΈ(HA)λ μ‘°μ§ μ¬μ λΆμΌμμ λ리 μ¬μ©λλ μ λ§ν μ¬λ£λ‘ μλ €μ Έ μλ€. λ§μ κ²½μ°μ, HAλ κΈ°μ‘΄ μ‘°μ§κ³Όμ κ²°ν©λ ₯μ μν₯μ λ―ΈμΉλ μ체μ ν©μ±μ μ¦μ§μν€κΈ° μν΄ κΈμ μνλνΈ νλ©΄μ μ½ν
μ μ©λλ€. κ·Έλ¬λ, νΉμ μ μ·¨μ±μΌλ‘ μΈν΄, HAλ 볡μ‘ν λͺ¨μμ κΈμ μνλνΈλ μ μ°ν κ³ λΆμ νλ©΄μ μ μ©λλλ°λ μ΄λ €μμ΄ μλ€. λ°λ‘ μ΄μ μμ, λ§μ΄ν¬λ‘ν¨ν°λμ΄ μ·¨μ±μ΄ μλ HAλ₯Ό νλ©΄μ μ μ©νλ λμμ΄ λ μ μλ€. ν¬ν 리μκ·ΈλνΌλ₯Ό μ΄μ©ν΄ μ μν HA λ§μ΄ν¬λ‘ν¨ν΄μ λ³Έ μ°κ΅¬μ§μ μλ‘μ΄ ν¨ν΄ νΈλμ€νΌλ§(Pattern transferring) κΈ°μ μ μ΄μ©ν΄ κ³ λΆμ νλ©΄μ λμ
λμλ€. μ΄λ¬ν λ°©λ²μ μ΄μ©ν΄, μ·¨μ±μ΄ κ°ν HAλ₯Ό κΈ°κ³μ μμ μ±μ κ°λ λμμ μ¦μ§λ μ체μ ν©μ±μ λΆμ¬νλ©΄μ μ μ°ν λ°μ΄μ€ κ³ λΆμ νλ©΄μ μ μ©νλ κ²μ΄ κ°λ₯νλ€.
첫λ²μ§Έ μ°κ΅¬μμ, λ³Έ μ°κ΅¬μ§μ μ§μ νμ HA ν¨ν΄μ μ€λ¦¬μ½ μ¨μ΄νΌ μμ μ μνκ³ μ΄ν κ·Έ ν¨ν΄μ νλΈ ννμ μΈκ³΅ νκ΄μΌλ‘ μ¬μ©λλ ν΄λ¦¬-L-λ½ν±μ‘μλ(PLLA) νλ¦μ νΈλμ€νΌνμλ€. μ체 νμ±μ κ°λ HA ν¨ν΄μ μμ²΄λ΄ νμ±μ΄ μ μ κ³ λΆμ μΈκ³΅ νκ΄μ μ체μ ν©μ±μ λΆμ¬νκ² λλ€. κ³ μ μ λ°λ ννμ ꡬ쑰λ₯Ό κ°λ HA ν¨ν΄μ΄ PLLA λͺ¨μ¬μ μ±κ³΅μ μΌλ‘ νΈλμ€νΌλμ΄ λ°νμλ κ²μ νμΈνμλ€. HA ν¨ν΄νλ PLLA νλ¦μ HAκ° μ½ν
λ PLLA νλ¦μ λΉν΄ μ°μν κΈ°κ³μ μμ μ±μ κ°λ κ²μ κ΅½ν, μΈμ₯, κ·Έλ¦¬κ³ μ체 λͺ¨λ°© μν 쑰건 μ€νμΌλ‘ μ μ μμλ€. λ°λΌμ μΌλ ¨μ μ€νμ ν΅ν΄ νλΈ ννμ μΈκ³΅ νκ΄μΌλ‘ μ μ©νλλ° μ ν©ν¨μ νμΈνμλ€. μΆκ°μ μΌλ‘, HA ν¨ν΄μ΄ νκ΄λ΄νΌμΈν¬μ ν¨ν΄μ λ°λ₯Έ μ¦μκ³Ό μ΄λμ μ΄μ§ν¨μΌλ‘μ¨ μ μν νκ΄λ΄νΌμΈν¬ν(endothelialization)μ μ λνμλ€. HA ν¨ν΄νλ PLLAμ νμ‘μμ μ±μ νλ©΄μ λΆμ°©λ μλμ μΌλ‘ μ μ μμ νμνμ ν΅ν΄ μ μ μμλ€. μ λ°μ μΌλ‘, HA ν¨ν΄νλ PLLAλ μ무 μ²λ¦¬κ° λμ§μμ PLLAμ λΉν΄ νλ₯ν κΈ°κ³μ μμ μ±λΏ μλλΌ, μ¦μ§λ μ체μ ν©μ±κ³Ό νμ‘μμ μ±κΉμ§ 보μ¬μ£Όμλ€.
λλ²μ§Έ μ°κ΅¬μμλ, PLLA 골νμ±μ λλ§(GBR λ©€λΈλ μΈ)μ μ μ©λ HA ν¨ν΄μ μ 2ν μ¬μ‘°ν©μΈκ°κ³¨νμ±λ¨λ°±μ§(rhBMP-2)λ₯Ό λ΄μ§νλ μλ‘μ΄ λ°©λ²μ κ°λ°νμλ€. PLLA λ©€λΈλ μΈμ μ±κ³΅μ μΌλ‘ λ§λ€μ΄μ§ HA ν¨ν΄μ νΈλμ€νΌνμκ³ κΈ°κ³μ μκ·Ήμ 쀬μλλ κ³ μ λκ³ μμ μ μΈ νμμ 보μ¬μ€μ νμΈνλ€. λ¨λ°±μ§μ΄ PLLAμ λΉν΄ HA ν¨ν΄ μμ μλμ λͺ
ννκ² λΆμ°©λμκ³ , HA μλμ λ΄μ§λ κ²½μ°μμ 7μΌμ κ±Έμ³ μμν λ°©μΆλλ κ²λ νμΈνμλ€. λ΄μ§λ rhBMP-2μ μμ HAμλ λ΄μ§λ μν(u/HAP)μμ κ°μ₯ λ§μκ³ , μ΄λ λ°κ΅¬ν ꡬ쑰λ₯Ό κ°λ HA ν¨ν΄μ λμ νλ©΄μ λλ¬ΈμΌλ‘ 보μλ€. μΈν¬ νμκ³Ό μ¦μ κ²°κ³Όλ€μ PLLA λ©€λΈλ μΈμ HAκ° λ―ΈμΉλ μ체μ μΈ ν¨κ³Όλ₯Ό μ¦λͺ
ν΄μ€λ€. μ 쑰골 μΈν¬μ λΆν(Osteoblastic differentiation) κ²°κ³Όλ 14μΌ λμμ ALP νμ±λμ λμ λλ μ¦κ°λ₯Ό ν΅ν΄ u/HAPμμμ rhBMP-2μ λ§μ λ°©μΆλμ μ
μ¦νλ€. λλ¬Ό μ€ν κ²°κ³Όλ 골μ¬μμ μ λνλ HAμ μν₯κ³Ό u/HAPμμ λ§μ μμ rhBMP-2κ° λ°©μΆλ¨μ λ°λΌ μ°μ₯λ 골μΉμ ν¨κ³Όλ₯Ό 보μ¬μ£Όμλ€.
κ²°κ³Όμ μΌλ‘, μ체 νμ±μ κ°λ HA ν¨ν΄μ΄ λ§λ€μ΄μ§κ³ PLLA νλ©΄μ νΈλμ€νΌλμ΄ μ±κ³΅μ μΌλ‘ μ체μ ν©μ±μ λΆμ¬νκ³ μΆκ°μ μΈ μ½λ¬Ό λ΄μ§ κΈ°λ₯μ λΆκ°νμλ€. μ΄μ κ°μ λ
νΉν ν¨ν°λκ³Ό νΈλμ€νΌλ§ κΈ°μ μ ν΅ν΄, μ·¨μ±μ κ°λ μ체μ¬λ£λ₯Ό μ μ°ν κ³ λΆμ λ©€λΈλ μΈμ μ μ©κ°λ₯ν κ²μΌλ‘ 보μ΄κ³ , μ΄μ κ°μ ꡬ쑰λ₯Ό λ€μν μ‘°μ§ κ³΅ν λΆμΌμ μ μ©ν μ μμ κ²μΌλ‘ κΈ°λλλ€.Hydroxyapatite (HA), a component of human bone tissue, is a promising material which is widely accepted in tissue regenerating applications. In many cases, HA is coated on metal implant surface to enhance biocompatibility for tissue fixation. However, due to its inherent brittleness, HA is hard to be applied on metal implants with complex shape or flexible polymer surface. Here, micro-patterning can be an alternative for surface application of brittle HA. HA micro-pattern fabricated by photolithography was introduced on polymer surface by our novel transferring technique. With this method, brittle HA can be applied on the flexible bio-polymer surface with mechanical stability, imparting enhanced biocompatibility.
In the first study, we fabricated a linear HA pattern on a silicon wafer and then transferred the pattern to a poly(L-lactic)-acid (PLLA) film for use as a tubular vascular graft. The bioactive HA pattern can impart biocompatibility on the bio-inert polymeric vascular graft. The HA pattern with its characteristic needle-like shape was successfully embedded into the PLLA. The HA-patterned PLLA film exhibited superior mechanical stability compared with that of a HA-coated PLLA film under bending, elongation, and in vitro circulation conditions, suggesting its suitability for use as a tubular vascular graft. In addition, the HA pattern guided rapid endothelialization by promoting proliferation of endothelial cells and their migration along the pattern. The hemocompatibility of the HA-patterned PLLA was also confirmed, with substantially fewer platelets adhered on its surface. Overall, in addition to good mechanical stability, the HA-patterned PLLA exhibited enhanced biocompatibility and hemocompatibility compared with pure PLLA.
In the second study, we developed a noble method to pattern HA loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2) on PLLA for GBR membrane. Successfully fabricated HA pattern was transferred to PLLA membrane with fixed and sound morphology in spite of mechanical stimuli. Protein was obviously attached on and under HA patterns contrary to PLLA and released gradually from under-HA in the long term of 7 days. The amount of loaded rhBMP-2 was overwhelmingly large on under HA sample (u/HAP), attributed to the large surface area of hemispherical HA pattern. Cellular morphology and proliferation results supported the biological effect of HA on PLLA membrane. Osteoblastic differentiation results proved the large amount of rhBMP-2 from u/HAP, representing remarkable increase in ALP activity after 14 days of culturing. Animal experiments demonstrated the prolonged bone healing effect with releasing larger amount of rhBMP-2 under the HA pattern.
In conclusion, bioactive HA pattern was fabricated and transferred on PLLA surface, successfully imposing biocompatibility and additional drug loading capability. With this unique patterning and transferring technique, brittle biomaterials are expected to be applied on flexible polymer membrane, which can be employed in various tissue engineering applications.Abstract i
List of Figures iv
Chapter 1. Introduction (Theoretical Review) 1
1.1. Composite biomaterials for tissue regeneration 2
1.2. Hydroxyapatite (HA) micro-patterning 4
1.3. Biological functions 5
1.3.1. Biological functions depending on pattern shape 6
1.3.2. High-dose drug loading reservoir 8
1.4. Aim of the studies 9
Chapter 2. Embedded HA micro-pattern for vascular tissue regeneration
13
2.1. Introduction 14
2.2. Experimental procedure 16
2.2.1. HA-patterned vascular graft fabrication 16
2.2.2. Characterization of HA pattern 17
2.2.3. Stability testing 18
2.2.4. In vitro studies 19
2.2.5. Platelet adhesion test 21
2.2.6. Statistical analysis 21
2.3. Results and discussion 22
2.3.1. Pattern on Si wafer 22
2.3.2. Pattern-transferred PLLA 23
2.3.3. Characterization of crystalline phases 23
2.3.4. Stability of HA-patterned PLLA 24
2.3.5. In vitro endothelialization on HA-patterned PLLA 26
2.3.6. Platelet adhesion tests 28
2.4. Conclusions 29
Chapter 3. High-dose drug loading system with HA pattern reservoir for guided bone regeneration 41
3.1. Introduction 42
3.2. Experimental procedure 44
3.2.1. HA-patterned membrane fabrication 44
3.2.2. Surface characterization 46
3.2.3. Stability testing 47
3.2.4. Protein release behavior 47
3.2.5. In vitro studies 48
3.2.6. In vivo animal experiments 49
3.2.7. Micro-CT analysis 50
3.2.8. Histological analysis 51
3.2.9. Statistical analysis 51
3.3. Results and discussion 52
3.3.1. Surface characterization 52
3.3.2. Mechanical stability 53
3.3.3. Protein release behavior 54
3.3.4. In vitro biological properties 56
3.3.5. In vivo animal experiments 58
3.4. Conclusions 60
Chapter 4. Conclusions 72
4.1. Conclusions 73
References 75
μ΄λ‘ (Abstract) 99Docto
A Study of the expert system for hazard and operability analysis in chemical processes
νμλ
Όλ¬Έ(λ°μ¬)--μμΈλνκ΅ λνμ :νν곡νκ³Ό,1996.Docto
The Relationship between Analytical Behavior of Moral Case and Moral Elevation in Youth
λλμ κ³ μμ κΈμ μ λλμ μλ‘μ νμΈμ λλμ μ νμ λͺ©κ²©νμμ λ κ°μ§κ² λλ μ μμ΄λ€. λ³Έ μ°κ΅¬λ λλμ μ¬λ‘μ μ μ νλ³ λΆμνλμ΄ λλμ κ³ μμ μ λ°νκ³ μΉμ¬νμ νλμ λκΈ°ννλλ° μ΄λ ν μν₯μ λ―ΈμΉλκ°μ λν μ°κ΅¬μ΄λ€. μ€νλμμ μ΄λ±νκ΅(N=71)μ μ€νκ΅ νμ(N=111)λ€μ λμμΌλ‘ νμμΌλ©°, μ€νκΈ°κ°μ 2κ°μ κ°μ΄μλ€. μ°κ΅¬λμμ μ΄λ±νκ΅μ μ€νκ΅ κ°κ° 3κ° νκΈμ λλμ μ¬λ‘λ§μ κ²½νν κΈμ λ°, λλμ μ¬λ‘μ λΉλλμ μ¬λ‘λ₯Ό κ²½νν κΈμ λΆμ λ°, λΉλλμ μ¬λ‘λ§μ κ²½νν λΆμ λ°μΌλ‘ ꡬλΆνμ¬ λλμ κ³ μκ²½νκ³Ό μΉμ¬νμ νλμ λκΈ°ν μμ€μ μ‘°μ¬νμλ€. μ°κ΅¬κ²°κ³Ό λλμ μ¬λ‘λ§μ κ²½νν κΈμ λ°μ΄ λΉλλμ μ¬λ‘λ§μ κ²½νν λΆμ λ°μ λΉν΄ μ λ°μ μΌλ‘ λλμ κ³ μμ λμ μμ€μ 보μμΌλ©°, λλμ νλμ λκΈ°ν μμ€λ λκ² λνλ¬λ€. κΈμ λΆμ λ°λ λΆμ λ°μ λΉν΄ κΈμ μ μΈ κ²°κ³Όλ₯Ό 보μλ€. λ°λΌμ κΈμ μ μΈ λλμ μ¬λ‘λ₯Ό λ§μ΄ κ²½νν μλ‘ λλμ κ³ μμ΄ μ¦μ§λλ©°, λλμ μ¬λ‘κ° νμ€κ°μ μν΄ λλμ κ°μ±μ μ λ°νλ λΉλλμ μ¬λ‘λ³΄λ€ λλμ μ΄κ³ μΉμ¬νμ νλμ νλλ° λ κΈμ μ μΈ μν₯μ λ―ΈμΉλ€κ³ ν μ μλ€. λ³Έ μ°κ΅¬μ κ²°κ³Όλ₯Ό κΈ°μ΄λ‘ λλκ΅μ‘ νλ‘κ·Έλ¨μ λλμ κ³ μμ μ¦μ§ν μ μλ κ΅μ‘λ΄μ©κ³Ό λ°©λ²μ κ°λ°λκ³ μ΄λ₯Ό μ€μ κ΅μ‘νμ₯μ λ°μν΄μΌ ν κ²μ΄λ€.Elevation is a positive moral emotion that elicited by witnessing acts of virtue. This study examined to find the relationship between witnessing a moral deed and experiencing elevation. The subjects of this study were students of an elementary school (3 class, N=71) and a girls middle school (3 class, N=111). Each of 3 class in elementary and middle school was a positive class which witnessing only moral act, a positive-negative class which witnessing a moral act and immoral act, and a negative class which witnessing only immoral act. The result showed that a positive and a positive-negative class were higher elevated than a negative class, and elevation motivated moral or prosocial behavior. This study suggested that educational materials and methods of moral elevation should be developed and applicated in the moral education program
μμ₯μ μ°©μμ λ°°μλ°λ¬μμ μ μ μμ λ°νκ³Ό κΈ°λ₯μ κ΄ν μ°κ΅¬
Thesis (doctoral)--μμΈλνκ΅ λνμ :μλͺ
κ³ΌνλΆ,2002.Docto
The Effects of Teacher`s Moral Behaviors, Witnessing in Elementary and Secondary School Days support on Positive Moral Emotion of College Students
λ³Έ μ°κ΅¬λ μ΄μ€λ±νκ΅ μμ κ΅μ¬μ λλμ νλμ μ§κ°μ μ μΌλ‘ 보μκ±°λ μ€μ λ‘ μνμλ‘μ κ²½ννμμ λ μκ°μ΄ μ§λ ν λνμμ΄ λμμ λ μ΄λ ν κΈμ μ μΈ λλμ μλ₯Ό μ λ°νκ³ λλμ νλμ λκΈ°ννλκ°λ₯Ό μμλ³΄κ³ μ νμλ€. κΈμ μ μΈ λλμ μμ λν μ°κ΅¬λ κΈμ μ¬λ¦¬νμ΄ λΆκ°λλ©΄μ μ΅κ·Ό λ§μ μ°κ΅¬κ° μ΄λ£¨μ΄μ§κ³ μλ€. λ³Έ μ°κ΅¬λ μ΅κ·Ό μμ μ΄ λκ³ μλ κΈμ μ λλμ μμ ν볡μ μμ μ°¨μ΄μ μ κ·λͺ
νκ³ μ νμλ€. κ·Έλ¦¬κ³ κΈμ μ λλμ μκ°μ μ°¨μ΄μ λ κ·λͺ
νλ €κ³ νμλ€. μ΄λ₯Ό μνμ¬ λ³Έ μ°κ΅¬λ λνμ μΈ κΈμ μ λλμ μμΈ κ³ μ, κ°μ¬, κ°νκ³Ό ν볡μ μμΈ κΈ°μ¨μ λνμ¬ κ°μ λ°μμ ν, μ 체μ κ°κ°, λκΈ°ν μμ€μμ λΆμνμλ€. κ·Έλ¦¬κ³ μ΄λ₯Ό λ°νμΌλ‘ μΈμ± λ° λλκ΅μ‘λ΄μ©κ³Ό λ°©λ²μμ μμ¬μ μ λμΆνλ €κ³ νμλ€. λ³Έ μ°κ΅¬μ κ²°κ³Όμμ κΈμ μ λλμ μλ ν볡 μ μμ ꡬλ³λλ μ μλ‘ μ
μ¦λμμΌλ©°, κΈμ μ μ μ κ°μλ ꡬλ³λλ νΉμ§μ 보μ΄λ κ²μΌλ‘ λνλ¬λ€. λ³Έ μ°κ΅¬λ₯Ό ν΅νμ¬ μΈμ± λ° λλκ΅μ‘μμ κΈμ μ μΈ λλμ μλ₯Ό λ°μν κ΅μ‘μ΄ νμνλ©°, κ΅μ¬μ λλμ νλμ΄ κ΅μ‘κ³Όμ μ λ°μλμ΄μΌ νλ€λ μ μ κ°μ‘°νκ³ μλ€. λν κΈμ μ λλμ μμμ λνλλ μ£Όμ κ°μ λ°μ, μ 체μ κ°κ°μ μΈμ± λ° λλκ΅μ‘μ νμ΅μ±κ³Όλ₯Ό νμΈνλ μ€κ±°λ‘ νμ©ν μ μμΌλ©°, κΈμ μ λλμ μ κ°μλ μνΈμ°κ³μ μΈ λμμ ꡬλ³λλ κ΅μ‘λ΄μ©μ κ°λ°νλ €λ λ
Έλ ₯λ νμνλ€κ³ 보μλ€.
The purpose of this study is to analyze the effects of teacher`s moral behaviors, witnessing in elementary and secondary school days support on positive moral emotion of college students. Recently, research on positive moral emotions has been studied as issues in positive psychology. This study was to investigate the differences between positive moral emotions and happiness. Also, this study tries to identify the difference between positive moral emotions. For this purpose, this study analyzed positive moral emotions, such as elevation, gratitude, admiration, and happiness emotion, such as joy in emotional reaction type, physical sensations, motivational effects. The result of this study proved positive moral emotions as distinct from happiness, and showed to difference between positive moral emotions. This requires curriculum about positive moral emotion in character and moral education. Also, this result suggest that the moral behavior of teachers should be reflected in the curriculum of character and moral education. In addition, emotional reactions and physical sensations of the positive moral emotions could be developed as tool to evaluate learning outcome of positive moral emotion education
A Research on Practical Cases of Multicultural Empathy EducationβFocused on Nonviolent Communication and Restorative Justice
νκ²½κ³Ό κ²½μ κ° ν¨κ» κ°λ μ§μκ°λ₯ν μ§μλ°μ μ μ± ν λ‘ ν (μ΄μ§μ©,κ°μΈκ΅¬,μμ±μ,μ±μ±μ£Ό,μκ΄ν¬,μ΄μκ·,λ°μν,μ€νλ ¬,μ΄μ©μ°,λ¨κ΄ν,μ΄μν,μ€λν,κΉμ’ μΌ,μ νλ)
β
. κ΅λ¦½μνμ 건립 λ°°κ²½
μ μΈκ³μ μΌλ‘ κΈ°νλ³νμ λ°λ₯Έ μ§κ΅¬?νλ°λμ νκ²½λ³νκ° μΌμ΄λκ³ μλ€. μ§κ΅¬μ¨λνμ μν κΈ°μ¨μμΉ, λΉνκ°μ, μ§μ€νΈμ°μ νν, κ°λκ³Ό μ¬λ§ν, ν΄μλ©΄ μμΉ λ±μ νμμ΄ μ¦κ°νκ³ κΈ°νλ³νμ λ°λ₯Έ μμμ§ κ°μ λ° μ‘μ λ° ν΄μμνκ³μ λ³νλ‘ λ©Έμ’
μκΈ°μ’
μ λν μνμμΈμ΄ μ¦κ°νκ³ μλ€. λν 무λΆλ³ν κ°λ°, λμν?μ°μ
νμ λ°λΌ μλ¬Όμ μμμ§ νκ΄΄, λ¨ν?ν¬νμΌλ‘ μΈν μλ¬Όλ€μμ±μ΄ κ°μνκ³ μλ€. μλ¬Όμμμ μ§μκ°λ₯ν κ΅κ°λ°μ μ ν λμ΄μ κ΅κ°κ²½μλ ₯μ ν΅μ¬μΌλ‘ 21μΈκΈ°μ κΈ°κ°μ°μ
μΌλ‘ λλνκ³ μμΌλ©° μλ¬Όμμμ κ΅κ°μμ κΆλ¦¬ λ° μλ¬Όμ’
μ£ΌκΆ ν보λ₯Ό μν κ΅κ°κ° κ²½μμ΄ λ λ‘ μΉμ΄ν΄μ§ μ λ§μ΄λ€.
- μ΄ν μλ΅νκ²½κ³Ό κ²½μ κ° ν¨κ» κ°λ μ§μκ°λ₯ν μ§μλ°μ μ μ±
ν λ‘ ν
?μ 1λΆ : μ§μκ°λ₯ν λμκ°λ° -μμ²μ Plan B
01.κ΅λ¦½μνμ 건립 μΆμ§λ°©ν₯ (μ΄μ§μ© νκ²½λΆ κ΅λ¦½μνμμΆμ§κΈ°νν νμ₯)
02.ν΄μμλ¬Όμμκ΄ κ±΄λ¦½ κΈ°λ³Έκ³ν λ° μΆμ§νν© (κ°μΈκ΅¬ ν΄μλΆ ν΄μμλ¬Όμμκ΄μΆμ§κΈ°νλ¨ νμ₯)
03.μμ²μ λΉμ κ³Ό μ§μκ°λ₯ν λ°μ (μμ±μ μμ²κ΅°μ² μ μ±
κΈ°νμ€μ₯)
?μ 2λΆ : μ§μκ°λ₯ν μ§μλ°μ μ¬λ‘μ κ²½ν
01.κ΅κ°κ· νλ°μ μ¬μ
κ³Ό μ°κ³ν μΆ©λΆμ μ§μλ°μ μ λ΅ (μ±μ±μ£Ό,μκ΄ν¬ μΆ©λΆκ°λ°μ°κ΅¬μ)
02.κ³΅κ³΅κΈ°κ΄ μ΄μ μ μ§μ νμ©λ°©μ (μ΄μκ· κ²½κΈ°κ°λ°μ°κ΅¬μ)
03.κ°μμ§μ λμ¬νκ²½μ λ¬Έμ μ κ³Ό κ°μ λ°©μ (λ°μν κ°μλ°μ μ°κ΅¬μ)
04.μμΈμ νκ°λ₯΄λ€μμ€ νλ‘μ νΈ (μ€νλ ¬ μμΈμμ κ°λ°μ°κ΅¬μ)
05.μ§μκ°λ₯ν λ³΄λ Ήλ°μ κ³Ό κ΄κ΄ -λ³΄λ Ήλ¨ΈλμΆμ λ₯Ό μ€μ¬μΌλ‘ (μ΄μ©μ° λ³΄λ Ήμμ² κ΄κ΄κ³Ό κ³Όμ₯)
06.μμ§μ€μΌμ΄λκ΄λ¦¬μ μ§μκ°λ₯ μ§μλ°μ -λꡬ경λΆμ§μμ μ€μ¬μΌλ‘
(λ¨κ΄ν λꡬ경λΆμ°κ΅¬μ λλνκ²½μ°κ΅¬μΌν° μΌν°μ₯)
07.곡μ
μ©μμ μμ§κ³Ό λΉμ© λ° κ³΅κΈμ²΄κ³ κ°μ (μ΄μν μΈμ°λ°μ μ°κ΅¬μ)
08.λλκ° νꡬμ§μμ νκ²½κ΄λ¦¬λ°©μ -λΉμ€ν±μ§λ μμ± λ° μ₯κΈ° λͺ¨λν°λ§μ μ€μ¬μΌλ‘
(μ€λν λΆμ°λ°μ μ°κ΅¬μ)
09.μμ°κ° νꡬμμ μ§μκ°λ₯ν λ°μ κ³Όμ μ μ λ΅ (κΉμ’
μΌ μ λ¨λ°μ μ°κ΅¬μ)
10.μ§μκ°λ₯ κ°λ°μ μν λμ μμ νκ²½μ λ΅ (μ νλ λμ λ°μ μ°κ΅¬μ