Recent advances in molecular imprinting-based ratiometric fluorescence sensors

荧光传感器具有灵敏度高、响应快、操作简便等优点;然而,在使用单发射荧光传感器时,背景信号、温度、p H值等因素都会对检测结果产生干扰.为使检测结果更灵敏、准确,具有自校正特性的比率荧光传感器应运而生.将模拟抗原抗体特异性结合作用的分子印迹技术引入到比率荧光体系中,即分子印迹比率荧光(molecular imprinting ratiometric fluorescence, MI-RFL)传感器,具有高选择、高灵敏及便捷性等特点,广受关注.本文梳理了2017年以来MI-RFL传感器构建与应用的研究进展.首先介绍了MI-RFL传感器的荧光源和工作机理,然后重点综述了能够提高传感器性能的印迹策略、荧光双/三发射类型,讨论了传感器的微型设计及在现场检测中的应用,最后,尝试提出了MI-RFL传感器面临的挑战,并展望了其发展前景

Recent advances in molecular imprinting-based ratiometric fluorescence sensors

荧光传感器具有灵敏度高、响应快、操作简便等优点;然而,在使用单发射荧光传感器时,背景信号、温度、p H值等因素都会对检测结果产生干扰.为使检测结果更灵敏、准确,具有自校正特性的比率荧光传感器应运而生.将模拟抗原抗体特异性结合作用的分子印迹技术引入到比率荧光体系中,即分子印迹比率荧光(molecular imprinting ratiometric fluorescence, MI-RFL)传感器,具有高选择、高灵敏及便捷性等特点,广受关注.本文梳理了2017年以来MI-RFL传感器构建与应用的研究进展.首先介绍了MI-RFL传感器的荧光源和工作机理,然后重点综述了能够提高传感器性能的印迹策略、荧光双/三发射类型,讨论了传感器的微型设计及在现场检测中的应用,最后,尝试提出了MI-RFL传感器面临的挑战,并展望了其发展前景

Microfluidic paper chip based on ionic imprinting coated quantum dot detection liquid sample and preparation method thereof

本发明属于材料科学与工程和微流控芯片技术领域，具体来说是一种基于离子印迹包被量子点检测液体样品的微流控纸芯片及其制备方法。检测液体样品的微流控纸芯片为通过酰胺键的成键方式和溶胶凝胶方式将离子印迹层包被量子点的结构固定于具有喷蜡Y型微流通道的玻璃纤维表面，即得检测液体样品的微流控纸芯片。本发明兼具快速、高效、便携、经济、高灵敏等优势，提供可以实现多种重金属离子同时检测的新策略，丰富了微流控芯片的相关研究

一种基于离子印迹包被量子点检测液体样品的微流控纸芯片及其制备方法

本发明属于材料科学与工程和微流控芯片技术领域，具体来说是一种基于离子印迹包被量子点检测液体样品的微流控纸芯片及其制备方法。检测液体样品的微流控纸芯片为通过酰胺键的成键方式和溶胶凝胶方式将离子印迹层包被量子点的结构固定于具有喷蜡Y型微流通道的玻璃纤维表面，即得检测液体样品的微流控纸芯片。本发明兼具快速、高效、便携、经济、高灵敏等优势，提供可以实现多种重金属离子同时检测的新策略，丰富了微流控芯片的相关研究

Rotary micro-fluidic cloth/paper composite chip for detecting lead and mercury ions and preparation method thereof

本发明公开了一种检测铅汞离子的旋转微流控布/纸复合芯片及其制备方法，其中，该复合芯片由底部传感器层、中间层、顶部取样层、铅离子印迹荧光传感布和汞离子印迹荧光传感布组成，前三者均由印有疏水图样的滤纸制成且从下至上依次叠装，底部传感器层上具有检测区，中间层的亲水通道与检测区匹配，顶部取样层的亲水采样区整体呈“V”字形且与亲水通道匹配，相邻两个亲水采样区之间开有探测孔；后两者分别由接有汞离子印迹或接有铅离子印迹的布块制成，均贴于底部传感器层的检测区且间隔排布。本发明开发出的旋转微流控布/纸复合芯片，很好地结合了纸芯片与布的特点，并且使用方便、能够特异性的灵敏检测铅汞离子

Recent advances in applications of fragment/dummy molecularly imprinted polymers

Molecularly imprinted polymers (MIPs) are designed to mimic the specific binding principle of enzymes to substrates or antigens to antibodies,while holding several advantages such as structure predictability,recognition specificity,easy preparation,low cost,high physical robustness,and thermal stability.Therefore,they have been widely applied in many fields including sample preparation (pretreatment),sensing analysis (chemo/biosensors),biomedicine,and environment/food analysis.To date,several strategies were developed for MIPs preparation,aiming to simplify the preparation process and/or improve the properties of the polymers,greatly broadening its usability.The exploration in various advanced imprinting strategies and their combinational use has become a research hotspot in MIPs preparation,among which the fragment imprinting strategy and the dummy template imprinting strategy are especially favored.Fragment imprinting,also called segment imprinting,uses a partial structure of the target molecule as a pseudo-template to prepare MIPs.This strategy is useful to target molecules that are not easy to obtain or that are too large to be used as templates,providing a feasible method for imprinting target analytes that are easy to inactivate or infect,as well as macromolecules that are difficult to imprint.In turn,dummy template imprinting uses molecules with structure,shape,and size similar to the target analytes as templates for imprinting.Because the target is not directly used as a template,this strategy can overcome problems of template leakage,as well as solve target molecule-related difficulties as they can be expensive,infectious,flammable,explosive,or chemically instable.This mini-review compiles information of several articles published in the last four years across ACS,Elsevier,RSC,and other databases,summarizing the most recent advances in the application of fragment/dummy template MIPs (FMIPs/DMIPs).Herein,the biomedical application of FMIPs is mainly addressed as a strategy for the detection of proteins and microorganisms,and the application of FMIPs in the field of food analysis is also explored.In recent years,the imprinting of mammalian cells has made some progress in the application of FMIPs.Mammalian cells,especially cancer cells,overexpress some proteins and sugars,which are good fragment templates.Consequently,the fragment imprinting strategy is widely used in cancer cell imaging,localization,and treatment.Moreover,due to the complicated structure and easy inactivation of some proteins,their MIPs are often prepared by fragment imprinting (also called epitope imprinting).As some microorganisms are infectious,imprinting microorganisms directly can pose a risk;therefore it is safer to also use the fragment imprinting strategy in such cases.The recent application of fragment imprinting strategy in other areas remains scarce.Nonetheless,three studies in the food analysis have explored this possibility.DMIPs are widely used in sample pretreatment and sensing analysis,and they are mainly used as SPE adsorbents for packed SPE,dispersive SPE (DSPE),magnetic SPE (MSPE),and matrix solid phase dispersion (MSPD) extraction.In addition,DMIPs are employed as molecularly imprinted membrane materials.As a result,by virtue of DMIPs,selective extraction and enrichment of target analytes from complicated samples can be achieved.MIP-based sensors can either recognize or transduce,meaning that they can specifically recognize and bind target analytes as well as generate output signals for detection.Because of the high selectivity of MIPs,the use of a dummy template imprinting strategy solves the problem of template leakage in the process of recognition and adsorption,further improving the detection accuracy and sensitivity of the sensor.These features expand the application range of MIP-based sensors

基于微流控的海洋监测技术

In the process of recognizing the laws of the ocean, developing the marine economy, protecting and restoring the marine ecological environment, marine analysis and monitoring are essential technologies. Modern ocean monitoring presents the characteristics of in-situ, on-site, and comprehensive, and requires analysis and monitoring equipment to be small, sensitive, rapid, and automated. Microfluidic chip technology, with its outstanding characteristics of micro channel control, low-cost, and high throughput, combined with advanced chemical analysis methods, has shown excellent prospects in the construction of core sensing components of marine analysis and monitoring equipment. In this review, starting from the manufacture of microfluidic chips and the construction of analysis methods, the typical objects of marine monitoring were classified, involving pH, dissolved oxygen, nutrients, heavy metals, organic pollutants and marine microorganisms. The progress of application research in monitoring was reviewed, and its future development was prospected

A QDs-based microfluidic device for the fluorescence detection of Cd~(2+) and Pb~(2+) ions based on ion imprinting technology

A novel quantums dots (QDs) based microfluidic chip device was proposed for the specific and sensitive detection of Cd~(2+) and Pb~(2+) . The glass fiber paper was activated by grafting with CdTe QDs to gain the fluorescence signal. Based on the ion imprinting technology,the Cd~(2+) and Pb~(2+) imprinted polymers (Cd-IIP and Pb-IIP) were synthesized by using APTES as a functional monomer and TEOS as a cross-linking agent on the surface of the glass fiber in paper. The paper@ QDs @ Cd- IIP and paper@ QDs @ Pb-IIP were applied for the detection of Cd~(2+) AND Pb~(2+) in lake sample. The proposed method indicated that the cadmium ion imprinting fluorescent sensor demonstrated a good linearity from 0. 05 to 80. 0 μg /L for Cd~(2+) with the limit of detection of 0. 01μg /L and 0. 10 to 78. 0 μg /L for Pb~(2+) with the limit of detection of 0. 03 μg /L. The recoveries of paper@ QDs @ Cd- IIP and paper @ QDs @ Pb-IIP were from 98. 0% to 104. 0% and from 99. 4% to 103. 4%, respectively. Furthermore,this method provides quantitative information of the detection of heavy metal ions in the environmental monitoring

Applications of Microfluidic Technology in Marine Analysis and Monitoring

In the process of cognizing the laws of the ocean, developing marine economy, protecting and restoring the marine ecological environment, marine analysis and monitoring are essential technology. Modern ocean monitoring presents the characteristics of in-situ, on-site, and comprehensive. Small, sensitive, rapid, and automated analysis and monitoring equipment is urgently required. Microfluidic chip technology, with its outstanding characteristics of micro channel control, low-cost, and high throughput, combined with advanced chemical analysis methods, has shown excellent prospects in the construction of core sensing components of marine analysis and monitoring equipment. This review described the materials and preparation of microfluidic chips and the construction of analytical methods, focusing on two types of applications, marine environmental analysis and marine in-situ online monitoring, involving pH, dissolved gases, nutrients, heavy metals, organic pollutants and marine microorganisms. This review summarized the application progress of microfluidic technology in marine analysis and monitoring, and provided an outlook on future development

The invention discloses a paper-based device based on a moving valve and a molecular imprinting technology, and a manufacturing method and application thereof

本发明提供了一种基于移动阀与分子印迹技术的纸基装置及其制作方法和应用，其特征在于，所述制作方法包括以下步骤：图样设计、芯片原件制作、电极在纸基上的构建、芯片原件连接、合成功能化分子印迹聚合物层、电化学聚合、洗脱模板分子；所述装置用于用于对血清样品中生物标志物的分析检测。本发明的有益效果在于：发明提供了一种基于移动阀与分子印迹技术的纸基装置及其制作方法和应用，为临床检测生物标志物提供了一种全新的装置，利用该装置进行原位合成生物标志物分子印迹聚合物，形成一套临床应用方法，开创了无抗体生物标志物分析的新策略，实现了在纸基装置上完成从装置制作到检测结果输出的全部过程，兼具经济、便携、快速等优势