黑果枸杞籽油品质及体外抗氧化活性Quality and in vitro antioxidant activity of Lycium ruthenicum seed oil

为挖掘黑果枸杞籽油的潜在开发利用价值，采用石油醚浸提法提取黑果枸杞籽油，对黑果枸杞籽油的理化性质进行测定，分析其脂肪酸组成及含量，并测定其对DPPH·、ABTS+·的清除能力和总还原能力。结果表明：黑果枸杞籽油碘值（I）为(84.0±0.2) g/100 g，皂化值（KOH）为(130.1±0.3) mg/g，过氧化值为(0.42±0.03) g/100 g，酸值（KOH）为(8.4 ±0.2) mg/g，需进一步精炼；黑果枸杞籽油主要包括亚油酸（68.31%）、油酸（17.76%）、棕榈酸（5.37%）、γ-亚麻酸（3.73%）、硬脂酸（2.58%）等9种脂肪酸；黑果枸杞籽油对DPPH·清除率的IC50为0.587 mg/mL，3.2 mg/mL黑果枸杞籽油的ABTS+·清除率为57.50%，且其具备一定的Fe3+还原能力。因此，黑果枸杞籽油富含不饱和脂肪酸，体外抗氧化活性良好，具有开发的价值和潜力。 In order to tap the potential development and utilization value of Lycium ruthenicum seed oil, Lycium ruthenicum seed oil was extracted by petroleum ether, and its physicochemical properties, fatty acid composition and content , DPPH· and ABTS+· scavenging power, and total reducing power were analyzed . The results showed that the iodine value, saponification value, peroxide value and acid value of Lycium ruthenicum seed oil were (84.0±0.2)gI/100 g, (130.1±0.3)mgKOH/g, (0.42±0.03) g/100 g, and (8.4±0.2) mgKOH/g, respectively, and it needed to be further refined. Lycium ruthenicum seed oil contained 9 kinds of fatty acids, such as linoleic acid (68.31%), oleic acid (17.76%), palmitic acid (5.37%), γ-linolenic acid (3.73%), and stearic acid (2.58%) and so on. The IC50 of Lycium ruthenicum seed oil for DPPH·scavenging rate was 0.587 mg/mL, the ABTS+· scavenging rate of 3.2 mg/mL Lycium ruthenicum seed oil was 57.50%，and it had a certain Fe3+ reduction power. Lycium ruthenicum seed oil is rich in unsaturated fatty acids, and it has good in vitro antioxidant activity, so it has development value and potential