Symphony: Optimized Model Serving using Centralized Orchestration

The orchestration of deep neural network (DNN) model inference on GPU clusters presents two significant challenges: achieving high accelerator efficiency given the batching properties of model inference while meeting latency service level objectives (SLOs), and adapting to workload changes both in terms of short-term fluctuations and long-term resource allocation. To address these challenges, we propose Symphony, a centralized scheduling system that can scale to millions of requests per second and coordinate tens of thousands of GPUs. Our system utilizes a non-work-conserving scheduling algorithm capable of achieving high batch efficiency while also enabling robust autoscaling. Additionally, we developed an epoch-scale algorithm that allocates models to sub-clusters based on the compute and memory needs of the models. Through extensive experiments, we demonstrate that Symphony outperforms prior systems by up to 4.7x higher goodput

11β-HSD1 inhibition ameliorates diabetes-induced cardiomyocyte hypertrophy and cardiac fibrosis through modulation of EGFR activity

11β-HSD1 has been recognized as a potential therapeutic target for type 2 diabetes. Recent studies have shown that hyperglycemia leads to activation of 11β-HSD1, increasing the intracellular glucocorticoid levels. Excess glucocorticoids may lead to the clinical manifestations of cardiac injury. Therefore, the aim of this study is to investigate whether 11β-HSD1 activation contributes to the development of diabetic cardiomyopathy. To investigate the role of 11β-HSD1, we administered a selective 11β-HSD1 inhibitor in type 1 and type 2 murine models of diabetes and in cultured cardiomyocytes. Our results show that diabetes increases cortisone levels in heart tissues. 11β-HSD1 inhibitor decreased cortisone levels and ameliorated all structural and functional features of diabetic cardiomyopathy including fibrosis and hypertrophy. We also show that high levels of glucose caused cardiomyocyte hypertrophy and increased matrix protein deposition in culture. Importantly, inhibition of 11β-HSD1 attenuated these changes. Moreover, we show that 11β-HSD1 activation mediates these changes through modulating EGFR phosphorylation and activity. Our findings demonstrate that 11β-HSD1 contributes to the development of diabetic cardiomyopathy through activation of glucocorticoid and EGFR signaling pathway. These results suggest that inhibition of 11β-HSD1 might be a therapeutic strategy for diabetic cardiomyopathy, which is independent of its effects on glucose homeostasis

Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients

Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report recovery of JEV GI live virus and identification of JEV GI RNA from cerebrospinal fluid (CSF) of encephalitis patients in JE endemic areas of China. Whole-genome sequencing and molecular phylogenetic analysis of the JEV isolate from the CSF samples was performed. The isolate in this study is highly similar to other JEV GI strains which isolated from mosquitoes at both the nucleotide and deduced amino acid levels. Phylogenetic analysis based on the genomic sequence showed that the isolate belongs to JEV GI, which is consistent with the phylogenetic analysis based on the pre-membrane (PrM) and Glycoprotein genes. As a conclusion, this is the first time to isolate JEV GI strain from CSF samples of encephalitis patients, so continuous survey and evaluate the infectivity and pathogenecity of JEV GI strains are necessary, especially for the JEV GI strains from encephalitis patients. With respect to the latter, because all current JEV vaccines (live and inactivated are derived from JEV GIII strains, future studies should be aimed at investigating and monitoring cross-protection of the human JEV GI isolates against widely used JEV vaccines

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

CRISPR/Cas9-Based Editing of Streptomyces for Discovery, Characterization, and Production of Natural Products

Microbial natural products (NPs) especially of the Streptomyces genus have been regarded as an unparalleled resource for pharmaceutical drugs discovery. Moreover, recent progress in sequencing technologies and computational resources further reinforces to identify numerous NP biosynthetic gene clusters (BGCs) from the genomes of Streptomyces. However, the majority of these BGCs are silent or poorly expressed in native strains and remain to be activated and investigated, which relies heavily on efficient genome editing approaches. Accordingly, numerous strategies are developed, especially, the most recently developed, namely, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system reveals remarkable higher accuracy and efficiency for genome editing in various model organisms including the Streptomyces. In this mini review, we highlight the application of CRISPR/Cas9-based approaches in Streptomyces, focus on the editing of BGCs either in vivo or in vitro, as well as target cloning of large-sized BGCs and heterologous expression in a genetically manipulatable host, for discovery, characterization, reengineering, and production of potential pharmaceutical drugs

Frequency-shifted dynamics of Nd:YVO4 laser with anisotropic and quite weak optical feedback

The dynamic response of single-mode a-cut Nd:YVO4 laser combined with linearly polarized oscillation and orthogonally polarized optical feedback is investigated. Under frequency-shifted of 2 MHz, the output laser exhibits amplitude and phase modulation with anisotropic feedback when arbitrary retarder is inserted in external cavity. Its response characteristics vary with both phase retardation and optical axis orientation of the retarder. The amplitude response coefficient (ARC) and the phase response coefficient (PRC) are introduced to describe the response characteristics. In the special case where phase retardation equal to π/2, there is the minimum value in ARC and jump in PRC. Based on the Jones matrix of birefringent external cavity and rate equations of micro-chip laser, theoretical analysis of the frequency-shifted dynamics is carried out, in which the amplitude and phase response coefficient bridge the measurement and simulation quite well. Under different feedback level, the response limit of micro-chip laser with anisotropic feedback is verified as low as 10-9 of the reinjected light

Effect of Different Dietary Patterns on Macronutrient Composition in Human Breast Milk: A Systematic Review and Meta-Analysis

The aim of this meta-analysis was to systematically review the relationships between lactation diet and the composition of mature breast milk through screening multiple databases and gray literatures, with priority given to quantitative articles published in Chinese and English. We identified 27 cross-sectional studies that included 4355 lactating women. According to these data, dietary patterns were categorized into four patterns based on the fat and protein energy supplying ratio, including rational-fat and rational-protein dietary (RR), high-fat and high-protein dietary (HH), rational-fat and high-protein dietary (RH), and high-fat and rational-protein dietary (HR). The results showed the fat content in mature milk was increased with increments of fat intake, while both the protein and lactose contents in mature milk did not increase with their intakes for lactating mothers. Among these four dietary patterns, the energy ratio of macronutrients in human milk at the RR was the closest to the estimated energy ratio for infants aged 0–6 M. In conclusion, our study represents that the rational dietary pattern should be advocated, and the irrational dietary patterns, especially high-fat and high-protein dietary patterns, should be avoided during lactation stage

Experiment investigation on vacuum arc of AMF contacts under different materials

In this paper, the arc characteristics of cup-shaped AMF contacts in vacuum arc experiments under different materials was studied. The experiment was conducted in a detachable vacuum chamber with a gas pressure of 10-4Pa. The arc voltage and current were measured at the same time by high-voltage probe and Rogowski coil, and the arc pictures were photographed with a high-speed camera. The cup and the rod of the contact are made of pure copper material, and the contact pieces are selected ten common contact materials for comparison (CuCr10, CuCr 20, CuCr 30, CuCr 40, CuCr 50, WCu10, WCu20, WCu30, CuW-WC). Fixed-distance trigger experiments were conducted with currents of 5kA (rms), 10kA (rms), and 15kA (rms) for each contact. The arc appearance, cathode surface morphology, and anode status morphology were observed. The shape of the arc was analyzed in conjunction with the voltage and current after the experiment. The morphology of cathode and anode surface was analyzed by combining the erosion after the experiment. The effect of various material properties was discussed under the AMF. It’s found that the center of the anode erosion area is approximately hexagonal, and the slot is burned more outward than the plane. WCu contact has better erosion resistance than CuCr contact. For CuCr, the erosion of CuCr30 is the slightest with low depth and small area