中泰中医教育体系的比较探析

培养高层次的医学人才是医学不断发展、创新的基础。随着人们对中医学的深入了解,运用中医治病防病已日益被人们所接受。中医疗法在当今的疾病防治和促进身心健康等方面均取得了举世瞩目的疗效。在泰国,由于中医药在防病治病和养生保健中的确切疗效,使其逐渐成为泰国医疗保健领域中不可缺少的部分。因此深入探讨中泰中医人才培养体系的异同,对系统认识中泰中医学具有重要的指导意义。分析比较两种中医学人才培养体系的异同,探求各自的特色,取长补短,可促进中泰中医学的创新和发展,同时也对世界传统医学的交融和发展具有积极的推动作用。中国中医科学院“十三五”重点领域研究专项第一批(No.ZZ10-011-1-2

电针促进胃黏膜损伤修复的时效关系及分子机制

目的:动态观察电针对胃溃疡模型大鼠胃黏膜损伤修复的影响,探讨电针治疗胃溃疡的时效关系和分子机制。方法:72只SD大鼠分为空白组、模型组、胃经穴组、对照点组,并按干预时间1、4、7 d分为3个亚组,每个亚组6只。采用乙醇灌胃方法制备胃溃疡大鼠模型,胃经穴组电针\"足三里\"\"梁门\",对照点组电针\"梁门\"\"足三里\"外旁开5 mm处,每日1次,每次电针30 min。空白组和模型组用鼠板束缚但不进行电针处理,每日1次,每次30 min。用逆转录-聚合酶链反应(PR-PCR)法检测胃增殖细胞核抗原(PCNA)、P物质(SP)的表达,Western blot检测胃神经降压素(NT)的表达。结果:干预1 d后,模型组溃疡指数显著高于空白组(P0.05),PCNA m RNA、SP m RNA低于模型组(P0.05)。干预7 d后,各组以上指标组间比较差异均无统计学意义(均P>0.05)。结论:电针胃经穴能在胃溃疡发展的不同病理状态下对PCNA m RNA、SP m RNA进行双向调节的平衡作用同时促进NT蛋白高表达,进而促进溃疡的修复。深圳市科技计划项目:JCYJ 20160406140612883;;国家自然科学基金项目:8147375

针药结合治疗湿热型痤疮的临床研究

目的:观察并对比针药结合治疗与常规西药治疗湿热型痤疮的临床疗效。方法:将100例湿热型痤疮患者随机分为针药结合组(n=50)和西药治疗组(n=50),针药结合组采用以口服中药为主并辅以针刺的疗法进行联合治疗,西药治疗组口服米诺环素胶囊,两组均连续治疗4周后观察疗效及皮损的类型、分级、评分和不良反应等。结果:针药结合组与西药治疗组总有效率分别为92. 00%、84. 00%,差异有统计学意义(P 0. 05。结论:针药结合疗法比常规西药治疗更能够显著提高湿热型痤疮的临床疗效。中国中医科学院“十三五”重点领域研究专项(No.ZZ10-011-1-2

An ecological study on zooplankton in the northern Beibu Gulf V: the effects of microzooplankton grazing on phytoplankton in summer

2011年8月份于北部湾北部海域5个观测站位获得的分层水样,分析了表层叶绿素A含量和表层微型浮游动物丰度以及类群组成;同时于现场采用稀释培养法研究了该海域浮游植物生长率(μ)和微型浮游动物的摄食率(g)。分析和测定结果表明:调查海区的微型浮游动物丰度400—1167个/l,类群组成以无壳纤毛虫为主;浮游植物的生长率为-1.50—1.13 d-1,微型浮游动物摄食率为0.33—1.08 d-1;推算微型浮游动物对浮游植物现存量以及初级生产力的摄食压力分别为28.1%—66.0%和-7.4%—438.4%。相对于中国其他海区,8月份北部湾北部海域微型浮游动物摄食速率处于中等水平。调查期间,广西沿海高生产力海区,浮游植物生长率大于微型浮游动物动物的摄食率,浮游植物生物量处于积累期;涠洲岛以南海域,浮游植物生产力较低,微型浮游动物摄食作用是控制浮游植物生长的重要因素。In August 2011,we quantified the impact of microzooplankton grazing on phytoplankton production in the surface waters of the northern Beibu Gulf,Vietnam.Shipboard dilution incubation experiments were carried out at five stations to calculate phytoplankton specific growth rates and the specific rates of grazing losses to microzooplankton.Dilution experiments using chlorophyll a( Chl a) as a tracer were used to estimate daily rates in two size fractions; image-analyzed microscopy provided quantitative estimates of microzooplankton standing stock( including ciliate and vertebrate larvae).PFW( particle-free water) was used to dilute seawater to five target dilutions of 0%,20%,40%,60%,and 80%.Microzooplankton grazing and phytoplankton growth rates were estimated by the linear regression of AGR( apparent growth rate) versus dilution factor.We estimated the grazing impact of microzooplankton on phytoplankton by calculating the percentage of phytoplankton standing stock and potential primary production ingested.Average total chlorophyll a concentration was( 0.67 ± 0.58) μg / L.Small-celled phytoplankton dominated this particular community.A notable characteristic of the phytoplankton community structure was that nano- and picoplankton made up a significant portion.Aloricate ciliates dominated the microzooplankton samples,in particular Strombidium,which accounted for 27.2% of total aloricate ciliate abundance.In addition,loricate ciliates and copepod nauplii were recorded.Microzooplankton densityvaried from 400 to 1167 ind / L.The horizontal distribution of microzooplankton was greater in nearshore than in offshore waters.Abundance did not differ significantly with chlorophyll a concentration in this study; however,there was a positive correlation between microzooplankton abundance and temperature.The phytoplankton growth rate( < 200 μm) ranged between-1.50 and 1.13 d-1,with the highest values being associated with nearshore waters.Meanwhile phytoplankton mortality due to microzooplankton grazing( 0.33—1.08 d-1) was highest at the same nearshore sites; however,growth rates were low at these sites.Consequently,microzooplankton consumed 28.1%—66.0% of chlorophyll a standing stocks and-8.1%—438.4% of the primary production per day.However,the grazing rate of microzooplankton on nanoplankton( 2— 20 μm) varied from 1.87 to 0.52 d-1,with an average of( 0.94±0.57) d-1.Given that the rates of grazing on phytoplankton of <200 μm were lower than those on nanoplankton,we conclude that microzooplankton prefer to graze on nanoplankton.There was a positive correlation between phytoplankton growth rate and grazing rate; in addition,significant differences between grazing rate and chlorophyll a were found.This suggests that microzooplankton grazing activity is the main factor regulating the community structure and restraining the growth of phytoplankton.During the investigation period, microzooplankton grazing effectively controlled the growth of phytoplankton,consuming 176.2% of primary production on average.When grazing rates are far greater than phytoplankton growth rates,energy is transferred from lower to higher trophic levels more effectively.In the surface waters of the northern Beibu Gulf,microzooplankton largely control the growth of phytoplankton by grazing offshore rather than in nearshore waters where primary production is high.The growth and grazing rates combined promote planktonic ecosystem stability in this area.These results indicate that grazing by microzooplankton is a key process controlling the growth of phytoplankton in this area.海湾公益项目(201005012

The relationship between nutrients and phytoplankton community structure in northern Beibu Gulf

根据2011年4月和8月北部湾北部(北部湾20°n以北的海域)的调查数据,北部湾北部春夏两季共鉴定出浮游植物312种,隶属于4门78属,硅藻是主要优势类群。春季优势种为细弱海链藻(THAlASSIOSIrA SubTIlIS)、丹麦细柱藻(lEPTOCylIndruS dAnICuS)、奇异棍形藻(bACIllArIA PArAdOXA)和尖刺拟菱形藻(PSEudOnITzSCHIA PungEnS);夏季优势种仅为海链藻(THAlASSIOSIrA SP.)。浮游植物群落在春季可划分为2个子群落(SPr1和SPr2),在夏季可划分为3个子群落(SuM1、SuM2和SuM3)。该海域n/P比值一直处于高的水平,绝大部分的水域表现为磷限制,这可能是硅藻大量生长所造成的。春季SPr2群落优势种的柔弱角毛藻(CHAETOCErOS dEbIlIS)与除铵盐之外的营养盐具显著相关关系,奇异棍形藻与环境因子无相关关系。夏季SuM3群落优势种的奇异棍形藻与磷酸盐具显著相关关系。分析表明,琼州海峡过道水为北部湾北部输送了磷酸盐,而硅酸盐则主要由陆地径流输入。Phytoplankton community and nutrient concentration in the northern Beibu Gulf in April and August 2011 were investigated.In this research, 312 phytoplankton taxa were identified, which belonged to four phyla and 78 genera, with Bacillariophyta being the main group.The dominant species were Thalassiosira subtilis, Leptocylindrus danicus, Bacillaria paradoxa, and Pseudonitzschia pungens in spring, and Thalassiosira sp.in summer.Phytoplankton community in northern Beibu Gulf could be divided into two natural groups in spring(spr1 & spr2) and three in summer(sum1, sum2 & sum3).N/P ratio was in a relatively high level in the study area, and most of the waters were phosphorus limitated which resulted from diatom blossom.Chaetoceros debilis was the dominant species of group spr1 and had significant correlation with nutrient in spring, except for ammonium.Bacillaria paradoxa was the dominant species of group sum3 and had significant correlation with phosphate in summer, but, it was uncorrelated with any environmental factors as the dominant species of group spr2 in spring.Phosphate was transported to northern Beibu Gulf through the Qiongzhou Strait corridor water, and silicate was input by land runoff.海洋公益性行业科研专项项目(201005012

Ecosystem structure and function in northern Beibu Gulf: zooplankton spatial niche and its differentiation

根据2006—2007年北部湾北部海域4个航次调查资料,采用k-优势度曲线法对浮游动物种(类)群进行排序分析,运用lEVInS公式和PIAnkA指数分析了主要优势种(类)群的生态位宽度值和生态位重叠程度,并通过除趋势典范对应分析(dCCA)研究生态位分化状况。结果表明,该海区浮游动物优势种(类)群按生态位宽度值可划分为广生态位、中生态位和窄生态位三大类型。其中,广生态位的种(类)群如肥胖软箭虫、亚强次真哲水蚤、长尾类幼体等具有较强的生态适应性,生活范围较广;窄生态位的种(类)群,对环境的适应性较差,对海流和水团等环境因素的变化具有指示意义。生态位宽度能够反映种(类)群丰度季节变化,但不能反映具体丰度大小。北部湾北部全年主要优势种(类)群的生态位宽度值介于0.08—0.77之间,并且各优势种(类)群的生态位宽度差异较大,种对间生态位重叠值介于0.02—0.89之间,平均生态位重叠指数为0.45,种(类)群之间利用资源环境的互补性较强,这与海域内生境多样化并且各优势种对环境适应能力的差异存在一定联系。dCCA分析表明,影响浮游动物分布的主要因子是水深、温度和盐度,其次是叶绿素A和溶解氧。The concept of ecological niche had remained as one of the core ideas in ecological research for almost a century,which was closely linked with interspecific competition,resource utilization had played an important role in the study of community composition and function,of the relationships among species,biodiversity,community succession and population evolution.Based on four seasonal investigations( Spring 2007,Summer 2006,Autumn 2007 and Winter 2006) in northern Beibu Gulf from 2006 to 2007,the niche breadth,niche overlap,spatial differentiation and environment effects on the abundance and spatial distribution of zooplankton were studied with qualitative and quantitative methods.The cumulative top 80% of zooplankton dominant populations were selected by the K-dominant curve method,then the niche breadths and their overlaps of zooplankton were measured using the formulas proposed by Levins Niche Breadth index and the Pianka Niche Overlap index,and the spatial niche differentiation was studied by using Detrended Canonical Correspondence Analysis( DCCA) method.The results showed that the zooplankton dominant species in northern Beibu Gulf were classified in to three groups,i e.,wide niche breadth groups( Bi= 0.60—0.77),middle niche breadth groups( Bi= 0.30—0.60),narrow niche breadth groups( Bi= 0.08—0.30) based on the niche breadth value.The niche breadths of zooplankton such as Sagitta enflata,Subeucalanus subcrassus and Macrura larvae were wider,which was related with their own characteristics and the capability of adapting environment,while the species whose niche breadths were narrower such as Doliolum denticulatum and Dolioletta gegenbauri will be considered as an indicative significance for ocean currents and water masses.Niche breadth could reflect the zooplankton abundance with the seasonal dynamic changes,and also could objectively reflect the species in the habitat in which the distributions of degree but it could not reflect the Accurate zooplankton abundance.The niche breadth values of dominance zooplankton species ranged between 0.08 and 0.77 in the whole year and The niche breadth difference among the dominant populations was great; The niche overlap values of dominance zooplankton species ranged between 0.02 and 0.89 in the whole year and the average niche overlap index was 0.45,there was strong complementarity in terms of the utility of resource among zooplankton populations,which had certain relationship with northern Beibu Gulf habitat diversification and the strong complementarity in terms of the utility of resource among zooplankton populations.The Detrended Canonical Correspondence Analysis result showed that depth,temperature and salinity had more important influence on the species spatial distribution than Chlorophyll a and dissolved oxygen.海湾公益项目(201005012); 我国近海海洋综合调查与评价专项(908-01-ST09

电针“梁门”-“足三里”对急性胃黏膜损伤模型大鼠血清代谢物动态表达的影响

目的采用核磁共振氢谱技术(1H NMR)探讨电针梁门-足三里治疗急性胃黏膜损伤的可能作用机制。方法 54只SD大鼠随机分为正常组、模型组、电针组,各组又分为干预1、4、7天3个时间点,每个时间点6只。除正常组外其余各组采用75%乙醇灌胃法复制急性胃黏膜损伤大鼠模型。造模成功后电针组大鼠用鼠板捆绑固定,采用疏密波(4 Hz,50 Hz),电针"梁门"(负极)"足三里"(正极),每次30 min,每日1次。正常组和模型组大鼠用鼠板捆绑固定30 min,每日1次。分别于干预1、4、7天后光镜观察胃黏膜病理形态,检测大鼠血清1H NMR谱,并利用模式识别方法分析不同干预时间下代谢物谱的差异,用正交偏最小二乘判别分析(OPLS-DA)模型和独立样本t检验,以P <0. 05筛选潜在的生物代谢标志物。结果病理结果显示,正常组大鼠胃黏膜表面细胞完整,未见溃疡性损害。干预1、4天时模型组大鼠可见胃黏膜上皮结构破坏严重,毛细血管增生及出血;干预4天时电针组大鼠胃黏膜的黏膜结构恢复较为完整,较模型组明显改善。血清代谢组学结果显示,正常组与模型组分离出差异代谢物6个:甘油磷酸胆碱、谷氨酰胺、甜菜碱、葡萄糖、乙酸盐和胆碱。与模型组比较,干预1天时电针组胆碱、甘油磷酸胆碱、葡萄糖、乙酸盐、谷氨酰胺和甜菜碱均升高;干预4天时胆碱、葡萄糖、谷氨酰胺和甜菜碱升高,甘油磷酸胆碱和乙酸盐降低;干预7天时甘油磷酸胆碱降低,葡萄糖、乙酸盐、谷氨酰胺和甜菜碱升高,胆碱无明显变化。结论电针"梁门"-"足三里"可有效回调急性胃黏膜损伤大鼠血清相关差异代谢物,恢复相应代谢通路稳定,以电针4天效果最佳,可能是其逆转胃黏膜损伤的作用机制之一。国家自然科学基金(81473751);;深圳市科技计划(JCYJ20160406140612883

三重疗法治疗雄激素性脱发疗效分析

目的:通过三重疗法治疗雄激素性脱发,并进行疗效分析。方法:66例雄激素性脱发病人随机分为试验组与针刺组,每组各33例。试验组采取针刺、梅花针联合实按灸疗法,针刺组采取针刺疗法。记录两组患者的疗效及伴随症状评分情况。结果:试验组的总有效率为81. 82%,针刺组的总有效率为57. 58%。两组的疗效比较差异具有统计学意义(P <0. 05)。治疗后,试验组的伴随症状评分均低于针刺组,差异均具有统计学意义(P <0. 05)。结论:电针、梅花针联合实按灸疗法治疗雄激素性脱发疗效显著,临床上具有推广及应用价值。中国中医科学院“十三五”重点领域研究专项第一批(No.ZZ10-011-1-2

中泰医学推拿比较系统探析

传统推拿学是中泰医学的核心内容之一,随着人们对推拿的深入探析,中泰按摩疗法在当今的疾病防治和促身心健康等方面均取得了举世瞩目的疗效。因此深入探讨中泰传统医学推拿体系的形成、操作方法和临床应用,对系统认识中泰推拿具有重要的指导意义。分析比较两种传统医学推拿体系的异同,探求各自的特色,取长补短,可促进中泰按摩的创新和发展,同时也为促进世界传统医学的交融和发展具有积极的推动作用。中国中医科学院“十三五”重点领域研究专项第一批(No.ZZ10-011-1-2

艾灸干预胃溃疡大鼠胃黏膜脑肠肽的动态表达

目的:观察艾灸干预胃溃疡大鼠胃黏膜相关脑肠肽的动态表达,探讨艾灸干预胃溃疡的时效关系。方法:SD大鼠72只随机分为3批,各批分为正常组、模型组、胃经穴位组和非经穴位组(n=6)。采用70%乙醇灌胃复制胃溃疡大鼠模型。胃(非)经穴位组的大鼠进行艾灸1、4、7d(30min/d)。干预结束后于光镜下观察胃黏膜形态,RT-PCR测定胃黏膜增殖细胞核抗原(PCNA)及P物质(SP)表达。结果:与正常组比较,模型组大鼠胃黏膜溃疡灶明显,胃黏膜PCNA表达降低(P<0.05),SP表达升高(P<0.05);与模型组比较,干预1d后胃经穴位组大鼠胃黏膜PCNA表达升高(P<0.05),干预4d后胃经穴位组PCNA表达升高(P<0.05),SP降低(P<0.05);干预7d后各组大鼠胃黏膜PCNA及SP表达无显著差异。结论:艾灸胃经穴位能有效调节相关脑肠肽指标,促进胃黏膜损伤修复,并具有一定的时效关系。深圳创新科技计划项目(No.JCYJ20160406140612883);;国家自然科学基金项目(No.81473751);;国家重点基础研究发展计划(973计划)(No.2015CB554502)~