Amplifying Non-Resonant Production of Dark Sector Particles in Scattering Dominance Regime

We investigate the enhancement of dark sector particle production within the scattering dominant regime. These particles typically exhibit a slight mixing with Standard Model particles through various portals, allowing for their generation through in-medium oscillation from Standard Model particle sources. Our analysis reveals that in the scattering dominance regime, with a significantly smaller scattering mean free path $\lambda_{\rm sca}$ compared to the absorption mean free path $\lambda_{\rm abs}$, the non-resonant production of sterile states can experience an enhancement by a factor of $\lambda_{\rm abs}/\lambda_{\rm sca}$. This phenomenon is demonstrated within the context of kinetic mixing dark photon production at a reactor, precisely satisfying this condition. By incorporating this collisional enhancement, we find that the current sensitivity to the mixing parameter $\epsilon$ for dark photons in the TEXONO experiment can be significantly improved across a range spanning from tens of eV to MeV. This advancement establishes the most stringent laboratory constraint within this mass spectrum for the dark photon. Sterile neutrino production, however, does not exhibit such enhancement, either due to the failure to meet the scattering dominance criterion or the neutrino damping in resonant production.Comment: 8 pages, 4 figure

PO-084 Research of HIIT Detraining on Mitochondria of Soleus Muscle Beclin1 and Bnip3 Contents in Aging Rats

Objective To observe the temporal variation of Beclin1 and Bnip3 protein in skeletal muscle aging degeneration by constructing the aged rat model, and to observe the effect of HIIT intervention on the changes of Beclin1 and Bnip3 protein and the relationship between the two. It provides a theoretical basis for the effect of exercise on the aging degeneration of skeletal muscle by affecting the level of mitochondrial autophagy. Methods 40 male Wistar rats aged 8 months were randomly divided into quiet control group (C) and HIIT intervention group (H). After the rats entered the animal room for one week of adaptive feeding and exercise, the rats in the C group did not exercise, and the H group exercise alternately based on the maximum oxygen uptake test results of the rats with the 70%-90%-50%VO2max intensity. Once every two weeks, the maximal oxygen uptake of the rats in group H and group C was tested. Group H underwent 50min/ days, 5 days / weeks, and lasted for 16 weeks. The rats in the two groups were randomly selected after the first VO2 test and eighth and sixteenth weeks after intervention. After anesthesia, blood was collected from the abdominal aorta and soleus tissue was obtained. The ROS activity in soleus muscle was tested by fluorescence enzyme labeling method. Isolation of mitochondria from soleus muscle using tissue mitochondria Isolation Kit, and the expression of Beclin1 and Bnip3 in the mitochondria of the soleus muscle was tested by Western blot. The Image Lab 4 software was used to collect the data of the protein test strip, and the SPSS 17 software was used to analyze the data. The results of the data analysis were presented in the form of mean standard deviation. In the process of protein strip analysis, the relative value of the protein content of each sample was obtained by the gray scale analysis method. The results of the first sampling were taken as the baseline value, and the ratio of the H group in the C group of 8 weeks and 16 weeks was obtained with the baseline value, that is, the relative value of the protein content. Then, repeated measurement of variance analysis was used to analyze the differences of different indicators at baseline level, 8 weeks and 12 weeks between group C and group H. The independent sample T test was used without interaction effect, and multivariate analysis of variance was used. A significant level of alpha =0.05 is set. Results (1) the content of ROS in skeletal muscle of rats was related to the process of natural aging (F=119.314, P < 0.001), and the level of ROS would rise with the process of natural collars (F=28.884, P=0.001; F=127.607, P < 0.001) through the comparison of the time points in the group C and the H group. At the same time, the level of ROS in group H was lower than that in group C, but there was no significant difference (P=0.310). And the interaction effect of time and exercise mode (HIIT) will not affect the result (F=0.814, P=0.477). But the growth rate of ROS in group H was lower than that in C group. ⑵Exercise, time change and their interaction did not affect the content of Beclin1 in rat skeletal muscle mitochondria (P > 0.05). ⑶The mitochondrial Bnip3 content in H group and C group was significantly different at 8 weeks (F=14.500, P=0.001), H group was significantly higher than that in C group, but there was no significant difference in mitochondrial Bnip3 content at the 16 week (F=0.090, P=0.767), and the Bnip3 content of skeletal muscle mitochondria changed with age (F=20.852, 0.001). The trend of H increased, but then decreased. There was a linear trend (F=6.950, P=0.005) between the level of mitochondrial Bnip3 content and the intergroup factors (time point changes) and the interaction between time and HIIT movement in rats. Conclusions  With the process of aging, (1) The content of ROS in skeletal muscle of rats increased significantly, while long-term HIIT training could delay the increase, but the best exercise time was unknown. (2) There was no obvious change in Beclin1 content in skeletal muscle mitochondria of rats, and HIIT training had no obvious effect on it. However, the changes in mitochondrial Beclin1 content relative to the total Beclin1 content of skeletal muscle need to be further studied; (3) The content of Bnip3 in skeletal muscle mitochondria in rats is increased, and long-term HIIT training has a delayed effect

Case report: Successful treatment of advanced pulmonary sarcomatoid carcinoma with BUBIB-ALK rearrangement and KRAS G12C mutation by sintilimab combined with anlotinib

Pulmonary sarcomatoid carcinoma (PSC) is a rare and aggressive subtype of non-small cell lung cancer (NSCLC) that is characterized by poor differentiation and invasiveness. According to the World Health Organization, PSC exhibits sarcoma or sarcomatoid differentiation and typically presents with an insidious onset, lacking specific symptoms and signs. It is associated with high malignancy, early metastasis, short survival time, and a poor prognosis. Treatment for PSC follows a similar approach to NSCLC; however, it presents significant challenges due to its high resistance to chemotherapy. Previous research has demonstrated the coexistence of two or more target mutations in PSC, and the presence of multiple mutations is correlated with higher mortality rates compared to single mutations. This is supported by our case study of a male patient with advanced BUBIB-ALK rearrangement and KRAS G12C missense mutation. There is currently no standard treatment protocol available for patients with this condition. The patient showed rapid progression after 1 month of alectinib treatment and was intolerant to paclitaxel + cisplatin chemotherapy. Following this, successful disease control was achieved with a combination therapy of sintilimab and anlotinib. The patient achieved a progression-free survival (PFS) of over 20 months, and long-term follow-up is still ongoing for the patient. Based on our clinical experience, the combination of anlotinib and programmed death-1 (PD-1) inhibitors may be a promising strategy for PSC patients, particularly those with multi-target mutations who do not respond to ALK-TKI and are resistant to chemotherapy

PO-098 Effect of HIIT on mitochondrial telomerase of skeletal muscle in aged rats: There is no full text article associated with this abstract

Objective The HIIT and moderate-intensity exercise are two different exercise models among the public fitness. In recent years, HIIT become more and more popular, unfortunately, there is a tremendous lack of research being done effects of mitochondrial reverse transcriptase (TERT) on age-related degeneration of skeletal muscle by HIIT. The purpose of this study was to compare the HIIT group and moderate-intensity group, and research difference of telomerase expression and cardiopulmonary endurance between the exercise group and the quiet control group was discussed. Methods  fifty-nine male Wistar rats were divided into three groups at random: control group (Q=19), moderate-intensity intervention group (M=20), and HIIT intervention group (H=20). The rats in Q group did not any exercise, and the rats in M group developed the exercise with 60% VO2max intensity for 8 weeks. H group did a training program for an 8-week exercise with alternating 40%, 60%, and 80% VO2max intensities. The rats in the experimental group were exercised for 50 minutes every day and trained for 5 days per week. After the baseline value group was sampled, each group of rats was selected after the training reached the specified number of weeks (4 and 8 weeks), and the maximum oxygen uptake test was performed before the material was taken. Single factor analysis of variance were used to assess differences in VO2max, and expression of protein between conditions. Results It was found that H group VO2max was significantly higher than M group and Q group (P<0.05). At same time, the mTERT expression of the M group at the 4th week was significantly higher than that of the Q group (P<0.05). The mTERT expression in group H was significantly higher than that in group Q at week 8 (P<0.05).There was no significant difference between the H group and the Q group at 8th week (P<0.05). Conclusions 1. HIIT exercise lasting for 8 weeks can effectively inhibit the decrease of maximal oxygen uptake in aging rats compared with moderate exercise. 2. HIIT training for 8 weeks promotes the expression of mTERT; 3. The maintenance of VO2max in aging rats may be related to the enhancement of mitochondrial antioxidant function by HIIT-promoted TERT to mitochondrial translocation

Tuberculosis burden in China: a high prevalence of pulmonary tuberculosis in household contacts with and without symptoms

Background: In the context of decreasing tuberculosis prevalence in China, we examined the effectiveness of screening household contacts of tuberculosis patients. Methods: A tuberculosis survey was conducted in 2008. All 3,355 household contacts of notified tuberculosis cases were examined with a questionnaire interview, chest X-ray and three sputum smear tests. The effectiveness was examined by comparing the prevalence of pulmonary tuberculosis in household contacts with or without presenting clinical symptoms against the respective notification rates. Regression models were used to evaluate the factors associated with pulmonary tuberculosis. Results: Of the 3,355 household contacts, 92 members (2.7%) had pulmonary tuberculosis, among which 46 cases were asymptomatic. The prevalence of pulmonary tuberculosis and smear positive cases in household contacts without symptoms were 20 and 7 times higher than the notification rates in 2008, while those in household contacts with symptoms were 247 and 108 times higher than notification rates, respectively. The patients detected were mainly Index Cases’ spouses, sisters/brothers and those who were in contact with female Index Cases. Conclusions: The present study provides convincing evidence that household contacts of notified tuberculosis cases are at higher risk of developing tuberculosis. Routine screening for household contacts without any symptoms is recommended for sustained tuberculosis control in China as well as in the world