Research and Development of Biopesticide in Supporting the Related Industrial Development (II)

本研究計畫主要目的在於以群體團隊力量發展生物農藥資源與專門技術,建立標的微生物製劑及其附加產品之量產與製劑系統,以支持並強化台灣本土性生物農藥產業的發展;在未來一年半計畫中擬進行之工作,大致有下列四項: 1. 病害防治用微生物及天然物配方之發展應用, 2. 真菌性殺蟲劑之發展應用, 3. 蟲生真菌綠殭菌(Nomuraea riley)之發展應用, 4. 建立黑殭菌(Metarhizium anisopliae)孢子自動分離及收集系統以供真菌性殺蟲劑之製作應用.擬發展之標的微生物包括Streptomyces sioyaensis PMS 502分離株、Gliocladium virens G8分離株、Trichoderma spp. YT3分離株、Pseudomonas putida YLFP14分離株、Verticillium lecanii、N. rileyi及M. anisopliae之MA-805與MA-126分離株.在量產含放線菌等標的拮抗細菌方面,將建立生產以休眠狀態菌體或含抗生物質培養液為主的液態發酵標準生產流程;針對標的真菌菌株之生產方面,二階段半固態發酵法將用以生產真菌之分生孢子,並進一步發展此些孢子之收集系統與劑型之改良,擬發展之劑型包括粉劑、乳劑及噴霧乾燥之粒劑,另一方面,篩選增強標的微生物製劑生物活性或延長其櫥架壽命之添加劑或方法亦為本研究計畫發展重點,此些試做產品樣本均將經生物活性、安定性以及所預期在田間實際施用之保護效果等測試.過去兩年來,本研究團隊部份研究成果中,已成功研發兩項病害防治用微生物製劑劑型,並已經農委會將建立好之技術移轉給2家國內私人產業進行商業化量產,在所擬具的未來延續工作中,發展成功一至二項可供技術移轉產業應用之生物農藥劑型將為吾人繼續努力之主要目標.The main objectives of this proposed team effort is to develop the resources and knowhows, and to establish ready-to-spin-off biotechniques and mass production and formulation systems to support and enforce the industrial biopesticide development in Taiwan. The proposed works to be carried out in the coming year are organized into four parts: 1. Development and practical application of microbial and natural products as disease control agents; 2. Development and practical application of fungal insecticides; 3. Development and practical application of green muscardine fungus Nomuraea rileyi; and 4. Establishment of automated spore separation and collection system for production of Metarhizium anisopliae as mycoinsecticides. The targeted microbial organisms include Streptomyces sioyaensis PMS 502 isolate, Gliocladium virens isolate G8, Trichoderma spp. isolate YT3, Pseudomonas putida isolate YLFP14, Verticillium lecanii, N. rileyi, M. anisopliae isolates MA-805 and MA-126. For the mass production of bacterial isolates (include the actinomycete members), standard operation protocol (SOP) for the liquid fermentation production of the resting stage biomass and/or antibiotic-metabolite-containing culture broth will be established. For the targeted fungal isolates, the semi-solid fermentation system will be established for the production of reproductive fungal spores; and the system for spore collection and formulation will be extensively explored. The attempted formulation includes powder, emulsion and spray-dried granules. Additives and methodologies effective in enhancing the biological activity of, and/or extending the shelf-life of the attempted product are both to be extensively explored subjects. All the attempted sample product will be tested for the biological activity, the stability, and as well the expected plant protection performance in practical application. During the past 2 years, efforts from part of this work team have successfully developed 2 biopesticide formulations useful for disease control. And the established techniques have been transferred to 2 local private enterprises for commercialization. In the continued effort proposed, additional 1 to 2 biopesticide formulation ready for technique transfer to private industry are the prime goal of the group effort