绿色荧光蛋白基因在大黄鱼体内的表达

以绿色荧光蛋白基因为报告基因 ,以pIRESneo为载体质粒 ,制备含有报告基因的质粒 ,以肌肉注射的方式导入大黄鱼体内 ,每隔一周用荧光显微镜观察绿色荧光蛋白基因在鱼体不同部位的表达情况 .结果表明 ,绿色荧光蛋白基因可以在大黄鱼体内得到表达 ,表达时间高达 2 8d以上 .绿色荧光蛋白基因不仅可以在肌肉注射部位组织细胞得到表达 ,而且在其它部位的肌肉、肝脏、肾脏和心脏等组织中也有表达 ,但表达水平低于肌肉注射部位组

Comparison of Recoveries of PAHs Obtained by Ultrasound-assisted Extraction from Wet and Freeze-dried Marine Sediment

以美国环保局(EPA)优先控制的16种多环芳烃(polycyclic aromatic hydrocarbons,PAHs)为研究对象,建立了湿法超声波辅助萃取技术提取沉积物中痕量PAHs的可靠方法,对萃取剂、萃取功率、萃取次数和萃取时间进行了优化。并与传统干法超声辅助萃取进行了比较,湿法超声波辅助萃取的PAHs回收率为57%～125%,而干法超声波辅助萃取PAHs的回收率为48%～113%,对相对分子质量小的PAHs如萘～芴的回收率,湿法明显高于干法。该文建立的湿法超声辅助萃取适用于沉积物和泥土中痕量PAHs的快速测定。An ultrasound-assisted extraction method for the extraction of 16 trace PAHs(USEPA priority pollutant) from wet(i.e.untreated) marine sediment was developed.The effects of solvent type,power of ultrasound,and extraction time were studied and optimized.The developed method was compared with the traditional ultrasound-assisted extraction for freeze-dried sediment.The results showed that recoveries of sixteen PAHs extracted from the wet marine sediment ranged from 57% to 125%,whereas those extracted from the freeze-dried marine sediment ranged from 48% to 113%.The results also indicated that the recoveries of PAHs with smaller relative molecular mass(e.g.naphthalene and fluorine) extracted from the wet sediment were higher than those from the freeze-dried sediment.Thus the developed method was appropriate for rapid determination of trace PAHs in marine sediment and soil.海洋公益性行业科研专项(200705011);; 中国海监技术支撑体系项目;; 2008年海洋环境保护及节能减排专

Low-temperature Preparation of Anatase TiO_2 Powders by Hydrothermal Method

[中文文摘]以钛酸四丁酯为前驱体,采用热液法在低于100℃的条件下制备了纳米晶TiO2粉体。运用DSC、XRD和HRTEM对所获得的TiO2粉体进行表征。XRD结果表明:所得到的TiO2粉体都是锐钛矿相,颗粒大小随着热液处理温度(60～100℃)的升高而增大,由Scherrer公式计算,其粒径介于4.8～6.9nm。同时,研究了所获TiO2粉体在紫外光下降解亚甲基蓝的性质。[英文摘要]Nanocrystalline TiO2 in the anatase phase has been obtained by hydrothermal method with tetrabutyl titanate below 100 ℃. The synthesized powders were characterized by differential scanning calorimetry (DSC), X-ray diffraction (XRD), and high resolution transmission electron microscope (HRTEM). XRD results show that the synthesized powders are all anatase. The grain size of the powders increases with the increment of the hydrothermal temperature from 60 degrees to 100 degrees, calculated grain size is from 4.8 to 6.9 nm by Scherrer method. The samples were investigated for the photodegradation properties of methylene blue (MB) solutions exposed to UV light illumination

凝固浴中加入碳纳米管对PAN碳纤维原丝结构性能的影响

在凝固浴中加入经酸处理的多壁碳纳米管(MWNTs),制得多壁碳纳米管-聚丙烯腈纤维,并对其进行了表征.结果表明:在凝固浴中加入酸处理MWNTs,对填补PAN碳纤维原丝内部的微孔有明显的作用;对PAN原丝的结晶结构几乎没有影响;降低了原丝的晶区取向;提高了原丝的断裂强度、断裂伸长率及拉伸韧度

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2015年5月至2015年12月笔者采用针刺听宫、后溪穴为主治疗颈型颈椎病28例,现报道如下。1临床资料28例均为厦门大学医学院国医堂针灸科和厦门大学附属中山医院厦禾分部的门诊患者,其中男12例,女16例;年龄20-55岁,平均（35±5）岁;病程最短1个月,最长2年;中医辨证属寒凝血瘀型18例,气血亏虚型10例。均符合《中医病证诊断疗效标准》（[1]）中颈型颈椎病的诊断标准,临床主要表现为颈肩背部酸痛、颈肌痉挛、僵硬,严重者活动受限

口服免疫添加剂对养殖大黄鱼免疫机能影响的初步研究

对福建省连江县厦宫乡新辉村的养殖大黄鱼病害进行免疫防治试验 ,通过定期口服免疫添加剂后 ,检测大黄鱼血清中溶菌酶活力、抗菌活力和酚氧化酶活力 ,测定大黄鱼体长和体重 .研究结果表明 :大黄鱼在服用免疫添加剂后血清中的溶菌酶活力平均值提高了 2 6% ,抗菌活力增加了 18% ,酚氧化酶活力提高幅度高达 4 6% .与此同时 ,实验组大黄鱼的生长速度也比对照组要快 ,其中实验组平均体长比对照组高 3.8% ,平均体重增加 13.5%

Concentration and distribution of polycyclic aromatic hydrocarbons in surface sediments collected in the Southern Yellow Sea

采用气相色谱与质谱联用(gC/MS)技术,在一个航次内对南黄海表层沉积物中16种优先监控的PAHS的污染状况进行了调查,采用菲/蒽、荧蒽/芘、荧蒽/(荧蒽+芘)、吲哚芘/(吲哚芘+苯并(g,H,I)苝)等特定比值对PAHS来源进行了分析.结果表明,南黄海表层沉积物中检出PAHS的总含量为90.4~732.65ng·g-1,各站点均以4~6环为主;与其它站位相比,倾废区的H01站位受到PAHS污染较为明显,无论是16种PAHS总量还是高分子量组分最高值都出现在该站点,虽然该海区沉积物中PAHS的含量没有超出生物影响低值,但苯并(b)荧蒽、吲哚芘和苯并(g,H,I)芘等一些没有最低安全标准的PAHS也有不同程度的检出,对海洋生物具有潜在的毒副作用.PAHS可能来源于原油、生物和煤燃烧造成的污染.The concentrations of sixteen representative Polycyclic Aromatic Hydrocarbons(PAHs) in surface sediment samples collected from the Southern Yellow Sea were determined by GC-MS.The distribution and potential sources of PAH contamination in the region were investigated.The types of PAHs found in the surface sediments were mainly 4～6 ring PAHs,and they are unevenly distributed among the different sampling sites covering an area of 139 km2.The area is known for continuous waste dumping activities in recent years,and indeed sites adjacent to such activities were found to have high levels of PAHs.Some high molecular weight PAH compounds such as benzo$$b$$fluoranthene,dibenzo$$a,h$$anthracene and benzo$$g,h,i$$perylene were detected in some samples.The health implication of these contaminants needs careful assessment since toxicity guidelines are not available for these species.The potential sources of PAHs are discussed using various source-specific PAH indexes such as Phenathrene/Anthracene、Fluoranthene/Pyrene、Fluoranthene/(Fluoranthene+Pyrene) and Indeno [1,2,3-cd] pyrene/(Indeno[1,2,3-cd] pyrene+Benzo [g,h,i] perylene).The composition of the observed PAHs reflects that they originate from both petroleum utilization and incomplete combustion.海洋公益性行业科研专项(No.200705011);中国海监技术支撑体系项目;2008年海洋环境保护及节能减排专项---

含副溶血弧菌(Vibrio parahaemolyticus) Fla I基因真核表达重组质粒的构建

用ClaⅠ /EcoRⅠ双酶切带有副溶血弧菌 (Vibrioparahaemolyticus)极鞭毛蛋白FlaI基因的质粒pMD18 FlaI,回收目的基因片段 ,插入到真核细胞表达载体pIRESneo同样经ClaⅠ /EcoRⅠ切开的窗口中 ,成功地将FlaI克隆到pIRESneo中 ,获得有意义的重组质粒pIRESneo FlaI.构建的pIRESneo FlaI真核表达重组质粒 ,将为海水经济鱼类副溶血弧菌DNA疫苗的研制奠定基

闽南养殖九孔鲍暴发性流行病的病原研究

从1999年春季东山县九孔鲍暴发流行病的病鲍内脏提取病原粗提液,经电镜观察和回归感染实验,证实其病原主要是病毒合并细菌感染,细菌为两种弧菌,病毒为球形,其发生基质为细胞质中一种双层膜的泡状结构,观察到病原入侵后引起鲍的组织细胞产生严重的病理变化,探讨病原的入侵途径,提出预防该流行病的一些措施