无酶光电化学法快速检测酒类中的乙醇含量

建立了应用于酒类中乙醇含量快速检测的无酶光电化学方法。通过水热法在FTO表面生长垂直板状WO3阵列（VAP-WO3）,利用SEM、EDX和XRD技术分别表征VAP-WO3的形貌、元素组成和晶格特征。光电化学实验结果表明,乙醇对VAP-WO3/FTO电极的光电流有明显增强作用。实验考察了钨酸钠反应量、偏置电压、缓冲溶液p H值对VAP-WO3/FTO电极检测乙醇光电性能的影响。在优化的条件下,乙醇的检测线性方程为ΔI=6.773+0.0494CEthanol（μg/mL）,线性范围为20.0160.0μg/mL,检出限为7.3μg/mL。此外,VAPWO3/FTO电极在乙醇检测中表现出良好的可重复使用性能和较高的选择性。将本方法应用于市场在售酒类中乙醇含量的检测,测定值与国标法的测定结果相符,并与酒精度的标注值相吻合,样品的加标回收率为93.9%107.0%。国家自然科学基金项目(Nos.21675062,21305050);;福建省自然科学基金项目(No.2017J05024)资助~

一种增/减量可变的计数式数控振荡器的设计

数控振荡器是全数字锁相环中的关键部件,目前应用较多的是除N计数式数控振荡器和增量/减量计数式数控振荡器,应用于锁相环时,前者做一次分频比调整就能使环路进入锁定状态,捕捉时间短,后者捕捉时间长,却有着前者没有的优势:结构简单、易于集成。提出了一种增/减量可变的计数式数控振荡器电路,此电路结构简单,同时也具有捕捉时间短的优点

Island instantaneous coastline extraction based on the characteristics of regional statistics of multispectral remote sensing image

本研究采用IkOnOS遥感图像,选取波谱特征区,通过区域像元统计并计算各波段权重系数,将影像进行波谱归一化处理,使归一化的影像岛陆与海水特征更明显。在此基础上,采用最大类间方差法确定最佳分割阈值,对归一化的影像进行二值化,从而提取海岛岸线。该方法采用特征曲线法进行水陆分离,得到的二值图像保持了原图的有效边缘,采用二值形态学提取的海岸线连续可靠、信噪比高。结果表明,该方法简单、快速,能有效提取海岸线,具有实用价值。This study adopted IKONOS remote sensing images and selected spectral characteristic areas, through regional pixel statistics and calculating weight coefficients of each band, processed the images with the spectral normalized method, which made the features of islands, land and water features more obviously in the images.On this basis, the OTUS was used to determine the optimal segmentation threshold, and the normalization image binarization was made, thus the island coastline was extracted.This method used the characteristic curve method to separate the land and water, obtained the binarization images and maintained the original edge effectively.The coastline that was extracted by Binary Morphology was continuous, reliable and high signal-to-noise ratio.The results showed that this method could extract the coastline fast, simply and effectively, which had the practical value

Analysis on the land use and cover change in Tianjin Binhai New Area based on the remote sensing

采用全国土地分类系统,基于lAndSAT卫星TM遥感影像与天津滨海新区1:50,000地形图,进行相应数据处理,编制出2000年、2005年、2010年三期滨海新区土地利用/覆盖分类图,并运用土地利用转移矩阵对近10年来天津滨海新区土地利用/覆盖变化进行定量分析。在此基础上,进一步分析了经济、人口等驱动因子对土地利用/土地覆盖的影响。结果表明:十年间,滨海新区的建筑用地面积增加显著,而绿地、湿地、滩涂等高生态价值的土地向建筑用地、未利用地等开发建设用地大幅度转化,后五年变化尤为显著。This paper carries out quantitative analysis on the land use/cover(LU/C) change of Tianjin Binhai New Area in recent 10 years through using land use transition matrix from the three-stage LU/C classification maps of 2000, 2005 and 2010 drafted by means of the National Land Classification System of China based on Landsat TM satellite remote sensing image and the Tianjin Binhai New Area 1:50 000 relief maps.On this basis, the impact of such driving factors as the economy and population on LU/C is further analyzed.The results show that the area of the building land in Binhai New Area has increased significantly over the ten years, and the greenland, wetland, and shoals of high ecological value have been dramatically transformed into the building land and unused land for the development and construction, and the change is more significant in the later five years

Cloning and Expression of the Leukotoxin Gene SH from Fusobacterium necrophorum

坏死梭杆菌白细胞毒素是坏死杆菌病的主要致病因子,白细胞毒素基因(lkT)是其编码基因。以分离到的国内牛源坏死梭杆菌fn(A)菌株f4基因组dnA为模板,应用PCr方法扩增白细胞毒素基因SH片段,克隆至PMd18-T载体上,以bAMHⅠ和HIndⅢ酶切的目的片段SH与相应酶切的PET32A载体连接构建PET32A-SH重组表达质粒,经转化E COlI bl21(dE3)后用IPTg进行蛋白诱导,SdS-PAgE检测重组蛋白表达情况。结果表明:扩增基因序列大小为1800bP,SdS-PAgE检测重组蛋白有效表达,表达得到大小为80.2kdA的目的蛋白,采用镍柱亲和层析方法纯化SH重组蛋白,获得了纯度达95%的重组蛋白;经WEST-Ern-blOT证实,该蛋白对抗坏死杆菌阳性血清具有反应活性。The leukotoxin of Fusobacterium necrophorum(FN) is considered to be one of the main virulence factors.The lkt gene encodes for FN.In this study,the SH fragment of lkt gene was amplified by PCR using the F4 genome as the template,which was isolated from the Chinese Fusobacterium necrophorum strain.The fragment was then cloned to the pMD18-T vector for sequencing.Thereafter,the SH fragment was subcloned into the multiple cloning sites of the pET32 to construct pET32a-SH recombinant plasmid,which was then trans-formed into E.coli BL21(DE3) with IPTG induction for expression.SDS-PAGE was used to analyze the recombinant protein.The results showed that the SH fragment of about 1800 bp was amplified and was about 80.2 kDa.The fusion protein was purified by Ni-NTA affinity chromatography under denature conditions,and their purity was above 95%.Western-blot analysis indicated the SH fragment had anti-genicity against Fusobacterium necrophorum.“十五”国家科技攻关子课题(2002BA518A04);吉林省科技发展计划项目(20070570);吉林市科技发展计划项目(200805

斑岩铜矿热液流体的kmg同位素示踪

文章系统分析了德兴斑岩铜矿热液蚀变岩的K-Mg同位素组成,发现蚀变斑岩和围岩的K-Mg同位素组成变化显著,其中δ~(41)K值为-1.02~0.38‰, δ~(26)Mg值为-0.49~0.32‰.除少量蚀变岩具有异常低的K-Mg同位素值外,大多数蚀变岩的K-Mg同位素值都高于相应的上陆壳基线值.这指示了在热液蚀变过程中,蚀变产物尤其是层状硅酸盐矿物优先富集重的K-Mg同位素.德兴斑岩铜矿蚀变岩的K-Mg同位素值与其K-Mg含量以及矿物学特征之间无相关性,可能指示了具有不同物理化学性质或同位素组成的流体作用.基于蚀变岩的K-Mg同位素特征,在德兴斑岩矿床中识别出了至少三种不同的热液流体.研究显示K-Mg同位素可用于示踪复杂热液体系中的不同地质流体

Research on integrated management and retrieval method of multi-source heterogeneous land basic data(多源异构土地基础数据一体化管理检索方法研究)

为了从多源异构的复杂土地基础数据中快速准确地提取用户所需信息，提出了基于元数据的一体化管理检索方法.在元数据信息提取、元数据加权索引、实体同义词扩展检索3个环节中，结合土地领域专业知识和用户实际需求，设计和开发了共享元数据表结构、加权元数据中字段相对重要性和信息熵因子，构建地名实体和专题数据层实体同义词库，并集成到包括中文分词、实体识别、同义词扩展、索引检索和相似度计算的一体化管理检索框架中，解决了多源异构土地基础数据统一管理和精确检索的问题.实践表明，该方法较传统的通用信息检索方法具有更好的适用性和更高的准确率

Crawler type walking mechanism with several walking modes

本发明公开一种多行走模式的履带式行走机构。具有结构相同的两个 模块，通过变形模块相互连接；其中一个模块结构为：主动带轮通过驱动 齿轮与第一电机的输出轴相连，从动带轮安装在机架上，履带围绕主动带 轮和从动带轮安装，第二电机的输出轴作为俯仰轴，与俯仰架一端安装一 起，齿轮安装在俯仰架的另一端，滚轮安装在俯仰架内，位于从动带轮和 齿轮之间。根据应用背景需要，组成直线型、并排型、复合型三种基本形 式，变形以适应环境与任务空间的要求