高粱总DNA导入春小麦新品系高分子量麦谷蛋白亚基的变化

通过花粉管通道法将高粱总DNA导入春麦甘麦8号、陇春13号和陇春10号,经过多代选择获 得了5个稳定遗传新品系．在高分子量麦谷蛋白亚基分析中,甘麦8号后代89144的高分子量麦谷蛋白 亚基发生突变,较其受体多了5+10亚基,而少了2+12亚基;其他几个转基因后代与其受体比较,高分 子量麦谷蛋白亚基组成未发生变化;但是各亚基的相对质量分数有较大变化．高分子量麦谷蛋白亚基 的组成和各亚基质量分数的变化直接影响小麦品质．本研究对外源总DNA花粉管通道法导入小麦在 改良小麦品质方面的作用进行了讨论

Magnetic resonance imaging of Fe3O4@SiO2-labeled human mesenchymal stem cells in mice at 11.7 T

<a href="mailto:Fe3O4@SiO2">Fe3O4@SiO2</a> core-shell nanoparticles were synthesized and used to label human mesenchymal stem cells (hMSCs) for in vitro and in vivo magnetic resonance imaging study. The diameter of the nanoparticles is 24-30 nm with a Fe3O4 core of similar to 8 nm and a SiO2 shell of similar to 8 nm. Transverse relaxivity of the nanoparticles dispersed in water is measured to be similar to 106 mM(-1) s(-1). After incubation with hMSCs for 12 h at a concentration of 100 mu g Fe/mL, cellular uptake of <a href="mailto:Fe3O4@SiO2">Fe3O4@SiO2</a> is 20-100 pg Fe/cell, which are located predominantly in the cytoplasm of cells. This level of uptake exhibits no significant influence on hMSCs&#39; viability and differentiation. In vitro imaging of <a href="mailto:Fe3O4@SiO2-labeled">Fe3O4@SiO2-labeled</a> hMSCs evenly distributed in agarose gel yields single cell sensitivity at 11.7 T. In vivo imaging of <a href="mailto:Fe3O4@SiO2-labeled">Fe3O4@SiO2-labeled</a> hMSCs injected into the left brain hemisphere of nude mice yields imaging sensitivity of similar to 130 hMSCs. (C) 2013 Elsevier Ltd. All rights reserved