Targeted antibacterial gene, polypeptide, recombinant protein and preparation method and application thereof

本发明提供了一种靶向抗菌基因、多肽、重组蛋白及其制备方法和应用，其中所述抗菌基因的核苷酸序列如SEQ ID NO.1所示，所述多肽的氨基酸序列如SEQ ID NO.2所示，所述重组蛋白含有抗菌VpBDef‑2基因，且表达抗菌VpBDef‑2多肽。本发明的有益效果在于：所述抗菌基因、多肽、重组蛋白可应用于生产蔬菜、畜禽及水产类抗菌药物、疫苗或饲料添加剂，具有广阔的应用前景；且所述基因、多肽、重组蛋白及其制备方法和应用可为进一步研究菲律宾蛤仔免疫防御机制提供基础，并为菲律宾蛤仔的病害防治、遗传选育和饲料添加剂研究奠定基础

Antifungal gene, polypeptide, recombinant protein and preparation method and application thereof

本发明提供了一种抗真菌基因、多肽、重组蛋白及其制备方法和应用，其中所述抗真菌基因为菲律宾蛤仔抗真菌Rpmacin基因，其核苷酸序列如SEQ ID NO.1所示；所述多肽的氨基酸序列如SEQ ID NO.2所示；所述重组蛋白含有抗真菌基因，且表达抗真菌多肽。本发明的有益效果在于：所述抗真菌基因、多肽、重组蛋白可应用于生产蔬菜、畜禽及水产类抗菌药物、疫苗或饲料添加剂，具有广阔的应用前景；且所述基因、多肽、重组蛋白及其制备方法和应用可为进一步研究菲律宾蛤仔免疫防御机制提供基础，并为菲律宾蛤仔的病害防治、遗传选育和饲料添加剂研究奠定基础

Inducing method and application of shellfish blood cell extracellular trap

本发明涉及一种贝类血细胞胞外陷阱的诱导方法。采用酵母多糖对贝类血细胞进行诱导，获得发生胞外陷阱的贝类血细胞。有益效果：将蛤仔血细胞用酵母多糖诱导，使得血细胞胞外陷阱的发生。本发明的方法能够有效诱导蛤仔血细胞发生胞外陷阱，并抑制细菌生长

胞外陷阱的免疫学研究现状及展望

Extracellular traps (ETs) are extracellular structures composed of chromatin and granule proteins that bind and kill microorganisms. Presently, most of the research on ETs was focused on the study of their inducing factors and antibacterial activities. However, the study on the formation mechanism of ETs, and the function of mitochondria in releasing ETs are still in its infancy. To gain insight into the study, function and action mode were performed to characterize the ETs formation mechanism and elucidate the changes of mitochondria preliminarily. Additionally, ETs could be degraded by DNA enzyme. Those above provides helpful evidence for revealing the mechanism of ETs formation, action mode and elimination, deepening the cognition on the innate immune defense in invertebrates, as well as extending the immunology boundary of marine invertebrates.</p

Prawn probiotics and application thereof

本发明涉及微生物领域，具体涉及一种对虾益生菌及应用。该菌株分类命名为贝莱斯芽孢杆菌Bacillus velezensis，于2020年6月8日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为：中国北京，保藏编号为CGMCC NO.20039；将上述菌株制备的饲料应用于大塘的养殖虾，大塘出虾产量提高了10‑15%

Bacterial strain for preventing bacterial diseases and application thereof

本发明提供了一种用于预防细菌性疾病的菌株及应用；该菌株的分类命名为侧孢短芽孢杆菌Brevibacillus laterosporus，该菌株已于2020年6月8日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为：中国北京，保藏编号为CGMCC NO.20038。将本发明中的益生菌添加到对虾饲料中，结果证实对虾感染副溶血弧菌和哈维氏弧菌后存活率提高，且对虾的生长速度和养殖水质均得到改善