Si,Ag,F離子共修飾HA納米生物薄膜的制備與表征

采用單電流階躍電化學沉積技術,在商業純鈦（CP-Ti）表面構建硅、銀、氟離子共修飾羥基磷灰石（Si-Ag-F-HA）納米復合薄膜。Ag+的持續釋放可以提供有效的抗菌性,Si 4+作為生物活性元素可以有效地抵消Ag+的潛在細胞毒性。采用電感耦合等離子體質譜法（ICP-MS）測定涂層中硅和銀元素的釋放規律。采用傅里葉變換紅外光譜（FTIR）、掃描電子顯微鏡（SEM）、能量彌散X射線譜（EDS）、X-射線衍射（XRD）等技術對得到的材料進行了表征。結果表明:Si,Ag和F三種元素均勻地摻雜到了HA的晶體結構中。Si-AgF-HA為納米級的針狀晶體結構,薄膜整體致密且均勻。Si-Ag-F-HA納米生物薄膜可以在一周內很好地誘導類骨磷灰石的形成,具有優異的生物活性。塔菲爾曲線測試結果證實涂層的耐SBF腐蝕性較好。ICP-MS測試結果表明Si-Ag-F-HA納米生物薄膜可以提供持續的Si和Ag離子釋放。FTIR和ICP-MS等光譜技術為開發新型抗菌硬組織修復材料提供了高效快速的檢測手段。In this study, a novel silicon, silver, fluorine co-modified hydroxyapatite (Si-Ag-F-HA) nano-biofilms was deposited on CP-Ti through electrodeposition. Ag was incorporated into HA coating to improve the antimicrobial properties. Si was added as a second binary element to offset the potential cytotoxicity of Ag. The as-prepared coatings were examined by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) tests. Results highlight that F-, Si4+ and Ag+ could be evenly incorporated into the Si-Ag-F-HA coating. The results indicate that the Si-Ag-F-HA coatings take the morphology of nanoscale- villous-like, the composite coating becomes more compact. The composite coatings were found to be bioactive, based on the promotion of additional apatite onto the Si-Ag-F-HA coating surface from SBF. Potentiodynamic polarisation tests revealed that the corrosion resistance increased after Si-Ag-F-HA coating. The release of Si and Ag ions from Si-Ag-F-HA coatings shows sustained release kinetics without burst release, which reached a near steady state afterwards, thereby revealing long-term sustainable release. FTIR and ICP-MS provide a rapid and effective method for the development of new antibacterial hard tissue repair materials