“海洋结构与装备的关键基础科学问题”研讨会学术综述

简要介绍了"海洋结构与装备的关键基础科学问题"研讨会的基本情况,对邀请报告分类进行了学术综述,对专家们的自由发言和集中研讨形成的研究对象及前瞻性关键科学问题进行了归纳总结

Study on expression and significance of tumor suppressor gene WWOX in hepatocellular carcinoma tissues by real-time fluorescent quantitative PCR

目的检测染色体普通型脆性位点FRA16D(16q23.3-q24.1)上WWOX(WW domain containing oxidoreductase)基因在原发性肝细胞癌(HCC)中的表达及意义。方法用RT-PCR、荧光定量PCR方法分别对40例原发性肝细胞癌癌组织(以下简称肝癌组织)、癌旁组织中WWOX基因的Δ6-8变异情况及其mRNA表达差异进行检测。结果WWOX mRNA在肝癌组织的表达(0.318±0.0559)显著低于癌旁组织(1.0),P<0.05;而且在肝癌组织中Δ6-8变异体的出现频率为20%(8/40),显著高于癌旁组织2.5%(1/40),P<0.05。结论WWOX基因的低表达、变异可能在肝癌的发生发展中起重要作用,并有可能作为肝癌的早期诊断指标之一。 【英文摘要】 Objective To evaluate the expression and significance of tumor suppressor gene(WWOX)in hepatocellular carcinoma(HCC)tissues.Methods Variant delta 6-8 of WWOX in patients with hepatocellular carcinoma was detected by RT-PCR and the expression of WWOX mRNA was examined by fluorescent quantitative PCR(FQ-PCR).Results The expression of WWOX mRNA in HCC patients(0.318±0.0559)was significantly lower than that in para-carcinoma tissue(P<0.05).Variant delta 6-8 was found in HCC and its frequency(20%,8/40)was sig..