DNA修饰电极的研究——Ⅶ.共价键合和吸附DNA-SAM/Au修饰电极的制备及表征

采用先通过 2 ,2′ 二硫二乙醇自组装得到自组装单分子层 (SAM) ,再在SAM上共价键合和吸附固定dnA的方法制备了两类dnA修饰电极 ,并对得到的dnA修饰电极进行了电化学和谱学表征 .结果表明该方法是可行的 ,也是较为理想的在电极表面固定dnA的方法 .国家自然科学基金!(批准号:39370213;39770220;29773034);厦门大学固体表面物理化学国家重点实验室基金;湖北省自然科

Electrochemical Engineering of Carbon Nanodots

作为零维碳基发光纳米材料,碳点是对现有发光纳米材料的重要补充. 精准控制粒径及表面结构对实现碳点的性质调控及其应用至关重要. 本文介绍了本课题组在利用电化学方法研究荧光碳点方面的进展. 重点展示了利用电化学方法实现对碳点粒径的控制,对表面氧化程度的调节以及对其发光机理的研究. 电化学方法可对只有几纳米厚度的材料表面进行有效的控制,可操作性强且经济环保. 通过对碳点的粒径及表面的调控,作者也进一步揭示了碳点的发光与表面结构的相关性. 这些工作为碳点的合成及其性质调控提供了可循的规律,有利于推动碳点在生物医生成像、传感检测、催化及能源转化等领域的应用.Carbon nanodots (CNDs), as zero-dimensional carbonaceous fluorescent nanomaterials, are valuable add-ons to the current cohorts of fluorescent nanoparticles. The fine control over the size and the surface is the key to gain designated photophysical properties of CNDs as well as empowers CNDs in many applications. Herein, a series of electrochemical strategies to manipulate the size and the surface of CNDs and to identify the surface structures was presented. Accordingly, the understandings on the originals of photoluminescence as well as the pathways of electrochemiluminescence of CNDs were revealed. These studies demonstrated that electrochemical methods were easy to operate, cost-effective and efficient in altering thin layers of the surface on CNDs within a few nanometers. The key findings in the luminescence mechanism provided guidelines for the rational design of CNDs with suitable features, which could promote applications of CNDs in bioimaging, sensing and catalytic conversion.通讯作者:庞代文E-mail:[email protected]:PANGDai-WenE-mail:[email protected].澳大利亚皇家墨尔本理工大学工程学院,墨尔本, 澳大利亚 30012.南开大学化学学院,分析科学研究中心,生物传感及分子识别天津市重点实验室,天津 3000711. School of Engineering, RMIT University, Melbourne, 3001, Australia2. State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, and College of Chemistry, Nankai University, Tianjin 300071, P. R. Chin

DNA-Modified Electrodes(Ⅸ)── Immobilization and Characterization of DNA Probes and the Molecular Hybridization on Gold Surfaces

采用巯基化合物自组装 /共价键合反应的逐层固定方法将双链 DNA固定到金表面得到 DNA修饰电极 ,并对该电极表面进行了电化学和 X射线光电子能谱表征 .研究了电极表面固定化 DNA的表面分子杂交 .对开发电化学基因诊断芯片和基因传感器具有一定意义Double stranded DNA was covalently immobilized onto gold surfaces by layer by layer immobilization method, and the surfaces were characterized by electrochemical and XPS methods. The surface molecular hybridization of DNA was studied on these functionized surfaces. This work might be fundamental for electrochemical gene chips and genosensors for disease diagnosis.国家自然科学基金! (批准号 :2 9773 0 3 4 ;3 9770 2 2 0 );; 国家杰出青年科学基金! (批准号 :2 0 0 2 5 3 11);; 教育部“高等学校

DNA-modified electrodes (IX) - Immobilization and characterization of DNA probes and the molecular hybridization on gold surfaces

Double-stranded DNA was covalently immobilized onto gold surfaces by layer-by-layer immobilization method, and the surfaces were characterized by electrochemical and XPS methods, The surface molecular hybridization of DNA was studied on these functionized surfaces. This work might be fundamental. for electrochemical gene chips and genosensors for disease diagnosis

Study on the Fluorescent Carbon Nanodots with Electrochemical Methods

作为以碳为骨架结构的新型纳米材料，碳点具有许多优良的性能，如发射波长可调、良好的光稳定性、抗光漂白、良好的水溶性以及易于生物偶联等. 正是因为这些优点，碳点和其它碳质纳米材料（富勒烯、碳纳米管、石墨烯）一样受到了广泛的关注. 电化学方法制备碳点具有条件温和、费用低廉、后处理简单等特点. 另外，电化学方法在材料的表面结构分析以及发光机理的研究中也有其独特的优势. 本文即就电化学方法在荧光碳点的制备以及发光机理探讨中的应用作了综述，并简略介绍了碳点在传感器中的应用，提出了优化电化学方法制备碳点的某些设想.Carbon nanodots are a new class of fluorescent nanoparticles with a carbon-based core, which possess wavelength-tunable luminescence, high photostability, resistance to photobleaching, water-solubility, and ease of bioconjugation. Owing to their attractive merits, carbon nanodots like other carbon nanomaterials such as fullerene, carbon nanotubes and graphene, have attracted much attention. In addition, carbon nanodots can be inexpensively produced by electrochemical methods with simple post-processing under mild conditions. Electrochemical approaches have unique advantages in the analysis of surface structures and luminescence mechanism of materials. In this review, recent advances in electrochemical methods used for the synthesis and luminescence mechanism of fluorescent carbon nanodots were summarized. The possible applications of carbon nanodots in biosensing are also included.武汉东湖新技术开发区“3551人才计划”（[2011]137）作者联系地址：武汉大学 化学与分子科学学院，教育部生物医学分析化学重点实验室，武汉生物技术研究院，湖北 武汉 430072Author's Address: College of Chemistry and Molecular Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine Ministry of Education, and Wuhan Institute of Biotechnology, Wuhan University, Wuhan 430072, China通讯作者E-mail：[email protected]