Matrine Induces Apoptosis of Hep G2 Cells via Mitochondrial Apoptotic Pathway

目的探讨苦参碱通过线粒体通路诱导HEP g2发生凋亡的机制。方法分别采用MTT法、PI染色法、流式细胞术检测苦参碱对肝癌HEP g2细胞的增殖抑制、周期调控、诱导凋亡作用;采用WESTErn blOT检测苦参碱诱导肝癌HEP g2细胞抑凋亡蛋白bId、bCl-2的表达量。结果苦参碱可抑制HEP g2细胞的增殖作用和诱导凋亡,呈时间和剂量依赖性,并可将HEP g2细胞周期阻滞在g0/g1期。苦参碱可引起线粒体膜电位崩溃,同时诱导bId、bCl-2表达下调,bAX表达上调。结论苦参碱通过调节细胞内bId、bCl-2和bAX蛋白的表达,经线粒体信号转导途径诱导人肝癌细胞系HEP g2发生凋亡。Objective To explore the mechanism of matrine inducing apoptosis of Hep G2 cells via mitochondrial apoptotic pathway.Methods MTT assay,PI staining,flow cytometry were used to evaluate the anti-proliferation,cell cycle regulation,and inducing apoptosis effects of matrine on Hep G2 cells.Western Blot assay was used to detect the expression levels of anti-apoptotic protein Bid and Bcl-2 in Hep G2 cells.Results Matrine could inhibit the proliferation and induce the apoptosis of Hep G2 cells in a time- and dose-dependent manner,and the cell cycle of Hep G2 was arrested in G0 / G1 phase.Apoptosis of Hep G2 cells induced by matrine was associated with collapse of mitochondrial membrane potential,which made the Bid and Bcl-2 expression levels down-regulated but the Bax expression level up-regulated.Conclusion Matrine induces the apoptosis of human hepatoma cell line Hep G2 via the mitochondrial apoptotic pathway by regulating the expression levels of Bid,Bcl-2 and Bax proteins.国家自然科学基金资助项目(81172284); 福建省自然科学基金资助项目(2010J0615

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies