Chemical Composition of Chinese Pyrola Herb Volatile and the Effects on the Osteoblast Proliferation

目的分析鹿衔草挥发油的化学成分,研究其对体外培养成骨细胞增殖的影响。方法采用水蒸气蒸馏法提取鹿衔草挥发油,GC-MS检测化学成分;鹿衔草挥发油干预成骨细胞系ROS17/2.8后,采用MTT法检测成骨细胞增殖,流式细胞术检测成骨细胞增殖周期,实时荧光定量PCR检测成骨细胞PCNA mRNA表达。结果一定浓度的鹿衔草挥发油能促进成体外培养成骨细胞增殖,处于增殖周期的成骨细胞比例明显增加,且成骨细胞的增殖细胞核抗原(Proliferating Cell Nuclear Antigen,PCNA)mRNA表达显著提高。结论鹿衔草挥发油通过上调PCNA表达,加速成骨细胞增殖周期进程,从而促进成骨细胞增殖。Objective To analyze the chemical composition of Chinese pyrola herb volatile and study its effects on the in vitro cultured osteoblast proliferation. Methods The water vapor distillation was used to extract Chinese pyrola herb volatile and GC- MS was to determine the chemical composition. After intervention of Chinese pyrola herb volatile on ROS17 /2. 8,MTT method was adopted to determine the osteoblast proliferation,the flow cytometry was to determine the osteoblast proliferating cycle and RT- PCR was to determine the expression of PCNA mRNA. Results Chinese pyrola herb volatile of a certain of concentration promoted the proliferation of in vitro cultured osteoblast cells. The percentage of osteoblast cells in the proliferation cycle was increased apparently and the expression of PCNA mRNA was improved significantly. Conclusion Chinese pyrola herb volatile accelerates the progression of osteoblast proliferating cycle through up- regulating PCNA expression so as to accelerate osteoblast proliferation.国家自然科学基金(81473706);; 福州市卫生系统科技计划项目(2013-S-wq10);; 福建省中医药科研项目(wzgs201307