Identification and Determination of Absorbed Components of Danggui-Shaoyao-San in Rat Plasma

Aim: To investigate the absorbed components of Danggui-Shaoyao-San (DSS) in rat plasma and their pharmacokinetics. Methods: Serum samples from rats with DSS extract administrated orally were analyzed by HPLC-DAD-MSn and an HPLC-DAD method was established for the determination. Results: Seven prototypes and one metabolite were identified in rat serum, and albiflorin, paeoniflorin, ferulic acid and ligustilide in rat plasma were determined for pharmacokinetic study. The proposed method was validated with a linear range of 0.025-5.60 μg·mL-1 and limit of quantitation (LOQ) at 0.022-0.180 μg·mL-1, respectively. The RSD of intra- and inter-day precision variations were less than 10.1% and the accuracies ranged from 92.5%-111.5%. The overall recovery was over 80%. Conclusion: Paeoniflorin sulfonate, albiflorin, paeoniflorin, ferulic acid, senkyunolide I, ligustilide and butylidenephthalide were found to be the potentially effective constituents in DSS. The method for determination is simple and sensitive, which is proved to be suitable for pharmacokinetic study of albiflorin, paeoniflorin, ferulic acid and ligustilide in DSS. © 2011 China Pharmaceutical University.目的:研究大鼠口服給藥當歸芍藥散后體內的入血成分及其藥代動力學。方法:大鼠灌胃給予當歸芍藥散提取物后,以HPLC-DAD-MSn分析含藥血清中藥物成分,并建立主要入血成分的HPLC測定方法以應用于藥代動力學研究。結果:大鼠灌胃給予當歸芍藥散提取物后血清中檢測到7個原型成分和1個代謝產物,并對大鼠血漿中芍藥內酯苷、芍藥苷、阿魏酸和藁本內酯進行了測定。方法學考察了線性范圍(0.025-5.60μg·ml-1)、定量限(0.022-0.18μg·ml-1)、日內和日間精密度(80%)。結論:芍藥苷硫酸酯、芍藥內酯苷、芍藥苷、阿魏酸、川芎內酯I、藁本內酯和丁烯基苯酞為當歸芍藥散主要的入血成分。對大鼠血漿中芍藥內酯苷、芍藥苷、阿魏酸和藁本內酯建立的測定方法簡便、準確、靈敏度高,適用于血藥濃度測定及其藥動學研究