An Exploration of Media Communication between Mainland China and Taiwan (1987-2008)

两岸关系在历史的洪流中起起伏伏数十年，但两岸媒体交流自1987年开启后就从来没有停止过。屈指算来，两岸媒体交流已经走过了二十二个年头。两岸交流发展至今，两岸媒体在及时、广泛、深入地沟通两岸关系的重要作用上仍是无法取代的。 本文试图通过对两岸媒体交流的历史梳理，探讨两岸媒体交流存在的现实问题。在两岸政治、经贸和文化交流日益密切的今天，在这种新的形势下，一方面，两岸媒体在交流中存在着哪些困境？是什么因素阻碍了两岸媒体的交流的进一步扩大化？两岸媒体应该在两岸关系中有怎样的角色定位，应该发挥怎样的功能？另一方面，本文也试图探讨在未来的媒体交流中，两岸应该采取怎样的合作方式进行交流和沟通，从而实现两岸...The relationship between mainland China and Taiwan is full of ups and downs for decades. However, media communication between mainland China and Taiwan has never stopped since 1987. In the past twenty-two years, cross-strait media communication has played an indispensable role in cross-strait exchange. Based on the history of the cross-strait media communication, the paper expects to explore ...学位：文学硕士院系专业：新闻传播学院新闻学系_新闻学学号：3062007115240

The time during which the map of each province in Shilinguangji was charted and the value of these maps

《事林广记》所附分省图是今见较早的中国古代系统的分区地图。本文从各幅分省图所载政区地名入手，结合《元史》、《新元史》、《大元一统志》、《大元混一方舆胜览》等文献中的相关记载，仔细考辨其建置沿革，分别考证各幅分省图所反映的地理面貌，就其绘制年代、所根据的地理信息与底图、史料价值等问题，形成了一些初步认识： （1）各幅分省图的绘制年代及其所据底图并不一致，然其最终汇集制图并刻版印刷，大抵不出延祐年间（1314—1320）；因此，总的说来，它们反映了元朝前期各行省的政区地理面貌。 （2）在各分省图中，“腹里图”、“汴梁行省所辖图”、“陕西行省所辖图”较多反映了金元之际的政区变动情形，“云南行省所...The provincial maps attached to Shi Lin Guang Ji（《事林广记》）are the earlier systematically district maps in the traditional China that can be seen today. This paper firstly discusses the names of administrative districts in the provincial maps. By using the records of Yuan Shi(《元史》), Xin Yuan Shi（《新元史》）、 Da Yuan Hun Yi Fang Yu Sheng Lan（《大元混一方舆胜览》）and so on, carefully research and analysis the establi...学位：历史学硕士院系专业：人文学院历史学系_历史地理学学号：1032008115229

日中両言語の待遇表現における人称代名詞の制約:一人称、二人称代名詞を中心に

关于日語第一人称代詞的使用,三輪指出“不用説`私が参ります,私にやらせて下さい'只要説`参ります,やらせて下さい'便足以表达意思。如果在此加上"ワタシ、オレ"这样的人称代詞反而显出説話人強烈的自我主張意訳。关于日語的第二人称代詞,金丸芙美指出"在日語里尽量不使用第二人称代詞,特別是対長辈,职位高的人不能使用"。"本稿就是計対达样的第一人称,第二人称代詞用法上的制約,从待遇表現的視点来分析日語和漢語里存在的相同点与不同点。departmental bulletin pape

日中両言語における人称代名詞の対照研究

http://purl.org/coar/resource_type/c_18w

現代中国語における「人」と「人家」の人称代名詞用法

在我们的日常生活中,有“不要随便拿人的东西"或“不要随便拿人家的东西"的两种表达方式。这里的“人"和“人家"所指的对象都是不特定的第三者。但是,在具有全面的品词分类的《现代汉语词典》里,“人家"被定为名词和人称代词,第二音节的“家"为一声时,为名词2轻声时为人称代词。而“人"只定义为名词,关于人称代词的用法却没有任何记载。在上述例子中,我们可以发现说话者和听话者,不包括在这里的“人"字之内。因此,笔者认为光把“人"定义为名词是否得当,还需进一歩考证。由于到目前为止,关于“人"的研究还不多见,本稿的日的是针对词典和先行 研究的不足,通过对“人"和“人家"的使用情况的对比,来考察两者在现代汉语中所处的位置以及作用。 通过考察,可以看到“人"和“人家"泛指“別人"时,用法基本一致,没有多大的差別。但专指“他"或“我"时,两者的动 机都是为了避免说“他"或“我",“人家"往往带有感情色彩,以表达说话者的主观情感。而“人"不带感情色彩,却能在待遇表现中起到委婉的作用。departmental bulletin pape

推荐一个化学生物学教学实验——电喷雾质谱研究磷酰基对丙氨酸与溶菌酶分子间相互作用的影响

介绍一个利用电喷雾质谱研究生物大分子与化学小分子相互作用的实验方法。讨论了该实验在化学生物学专业本科生教学工作中的实践效果

雷帕霉素衍生物对胰岛的毒性研究

目的探讨雷帕霉素及其衍生物依维莫司、地磷莫司、佐他莫司对胰岛的毒性作用。方法采用小鼠胰岛素瘤细胞(MIN6)作为体外研究胰岛细胞的对象,分别在含有依维莫司、地磷莫司、佐他莫司的培养基中孵育MIN6细胞48小时,通过Brdu检测细胞增殖、CCK8检测细胞活力、PI检测细胞周期、流式细胞术检测细胞凋亡以及ELISA检测细胞分泌胰岛素功能,观察3种雷帕霉素衍生物对MIN6细胞的影响。结果我们发现3种雷帕霉素衍生物均会对MIN6细胞的增殖和活力产生抑制作用。在细胞周期和凋亡实验中,与阴性对照组比较,3种衍生物对MIN6细胞的影响呈现抑制G1期向S期转变的趋势和促进细胞凋亡的趋势,但差异没有统计学意义。另外,3种衍生物与雷帕霉素一样,均可减少MIN6细胞分泌胰岛素,差异具有统计学意义。结论雷帕霉素及其衍生物依维莫司、地磷莫司、佐他莫司均对胰岛产生一定毒性。国家自然科学基金项目(31271038,81302546

Promotion of proliferation of luminal B breast cancer cells by mesenchymal stem cells and its underlying molecular mechanisms

目的分析人脐带间充质干细胞(hUC-MSCs)对Luminal B型乳腺癌细胞生长增殖的影响,并初步探讨其可能的分子机理。方法绿色荧光蛋白(GFP)和荧光素酶共表达慢病毒感染人Luminal B型乳腺癌细胞BT474,并经嘌呤霉素筛选两周后,于荧光显微镜下观察GFP的表达情况,IVIS Kinetic成像系统拍照以观察和记录慢病毒感染后BT474细胞荧光素酶的表达情况;荧光显微镜下直接观察,结合MTS实验分析hUC-MSCs共培养或其浓缩上清处理对GFP和荧光素酶共表达BT474细胞生长增殖的影响;Western blot法检测hUC-MSCs浓缩上清处理对BT474细胞Akt和MAPK信号通路激活情况以及下游细胞周期调控蛋白Cyclin D1表达的影响;常规RT-PCR法检测hUC-MSCs中NRG-1、NRG-2、IGF-Ⅰ、IGF-Ⅱ和EGF等配体的表达。荧光素酶表达强度与细胞数量的相关性经由Excel软件行统计学分析,MTS实验数据则经由SPSS13.0统计软件行统计学分析。结果荧光显微镜和IVIS Kinetic成像系统的观察结果分别证实,GFP和荧光素酶经慢病毒载体系统的介导可在BT474细胞中成功地共表达,且荧光素酶的表达强度与细胞数量呈直线相关。MSCs共培养或其浓缩上清处理均可显著促进Luminal B型乳腺癌细胞BT474的生长增殖,其细胞存活比例分别为各自对照组的148.06%(P<0.005)和147.99%(P<0.001);MSCs浓缩上清处理同时激活BT474细胞内Akt和MAPK信号通路,并上调细胞周期调控蛋白Cyclin D1表达。此外,RT-PCR结果显示,hUC-MSCs中NRG-1和EGF的mRNAs水平呈高表达,而NRG-2、IGF-Ⅰ和IGF-Ⅱ等配体的mRNAs表达也可见。结论 MSCs可通过表达并分泌NRG-1等配体,从而激活Luminal B型乳腺癌细胞BT474的下游Akt和MAPK信号转导通路以上调细胞周期调控蛋白Cyclin D1的表达,进而促进其生长增殖。Objective To investigate the effect of human umbilical cord mesenchymal stem cells(hUC-MSCs) on proliferation of luminal B breast cancer cells and its underlying molecular mechanisms. Methods Human luminal B breast cancer cells BT474 were infected with GFP and luciferase co-expressing lentiviruses and then subjected for selection with Puromycin for 2 weeks. The expression of GFP and luciferase was detected by fluorescent microscopy and IVIS Kinetic image system, respectively. The effect of coculture or treatment with conditioned medium of hUCMSCs on proliferation of BT474 was analyzed with MTS assay. Western blot was carried out to detect the effect of treatment with conditioned medium of hUC-MSCs on the activation of both Akt and MAPK signalings in BT474, as well as the expression of downstream cell cycle regulator Cyclin D1. Regular RT-PCR was applied to analyze the mRNAs expression of ligands such as NRG-1, NRG-2, IGF-Ⅰ, IGF-Ⅱ, and EGF in hUC-MSCs. The correlation between relative luciferase activity and cell number was analyzed with Excel software, while MTS assay data was statistically analyzed with SPSS 13.0 software. Results The co-expression of GFP and luciferase in BT474 via lentiviral expression system was visualized by fluorescent microscopy and IVIS Kinetic image system. The linear correlation between relative luciferase activity and cell number was determined by curve fitting analysis. Coculture or treatment with conditioned medium of hUC-MSC significantly promoted the proliferation of BT474, with survival rates being 148.06 %(P < 0.005)and 147.99 %(P < 0.001)of control, respectively. In addition, treatment with conditioned medium of hUC-MSC was shown to induce activation of both Akt and MAPK signalings, which further upregulated the expression of Cyclin D1. Moreover, high mRNAs expression levels of both NRG-1 and EGF, as well as moderate mRNAs expression levels NRG-2, IGF-Ⅰ, and IGF-Ⅱ were showed by RT-PCR. Conclusion Our results here demonstrated that MSCs may promote the proliferation of luminal B breast cancer cells through paracrine of ligands such as NRG-1, which in turn results in the activation of both Akt and MAPK signalings and upregulation of the expression of Cyclin D1.国家自然科学基金面上项目(81272922);; 福建省自然科学基金面上项目(2016J01577);; 福州总医院院内课题国际合作研究专项(2015G01

Evaluation technique of the loss of coastal ecosystem services caused by sea reclamation project with three methods

在分析填海工程对海洋环境影响的基础上,结合《海洋生态资本评估技术导则》(gb/T 28058-2011)综合构建了填海工程对海洋生态系统服务功能损耗评估指标体系。分别采用价值评估法、能值分析法和物种丰度经验公式法对生态损耗进行价值评估,以福鼎市塔下新建填海工程为分析对象进行了实证评估,生态系统服务价值总损失量分别为672.0万元·年–1、809.2万元·年–1和133.3万元·年–1。通过与国内相关研究成果进行比较,分析三种评估方法的可行性和适用性,以期在实际环境影响评价工作中将工程外部成本纳入项目经济损益分析,为海岸带经济可持续发展提供技术支撑。Based on the effects of marine environment caused by sea reclamation and Technical Directives for Marine Ecological Capital Assessment(GB/T 28058-2011), the indexes of the losses of coastal ecosystem services caused by sea reclamation were built.The lost value of sea reclamation was calculated with three methods including economic evaluation, emergy analysis and biodiversity experiential formula which were applied in the new sea reclamation in Taxia Fuding Fujian Province separately, and the total ecosystem lost value was 672.0×104yuan·a–1, 809.2×104yuan·a–1, and 133.3×104yuan·a–1 correspondingly.Compared with other relevant researches, the methods are expected to apply in EIA economic evaluation in order to consider external cost and to apply in coastal economic sustainable development.海洋渔业安全环境保障服务系统关键技术研究及示范应用(201205006

有限要素法を用いた付着強度の寸法効果に関する解析的考察

application/pdfjournal articl