Isolation of Japanese Encephalitis Virus from Mosquitoes Collected in Omura District, Nagasaki Prefecture, Japan, in 1964.

1964年5月26日から10月13日に至る間大村市近郷黒丸部落で捕集した13442個体144プールのコガタアカイエカから19株の日本脳炎ウイルスが分離された.このうち3株は分離当初ヒナ血球に対する凝集能が甚だ低くかったがマウス脳内5代通過後,凝集能が認められJEウイルスと同定された.最初のウイルスは6月8日分離され従来日本各地におけるウイルス分離時期に比べ甚だ早いことが注目された.オトリ動物として使用したウサギの抗体上昇の時期は患者発生に先立つこと1週又は1ケ月前に認められ,県下の患者発生状況と比較しウイルス汚染の程度を推察することが出来た.Isolation of Japanese Encephalitis virus from mosquitoes collected at Kuromaru village in Omura city Nagasaki prefecture had been made on May 26th thru October 13th 1964. The total 19 arboviruses could be isolated from 13442 in 144 pools of Culex tritaen

Epidemiological Studies on Viral and Parasitic Diseases, and Vector Insects in East Africa : I. Virological Studies, Especially on the Epidemiology of Arboviruses (Preliminary Report)

Virological investigations have been made in Mwanza, Republic of Tanzania, East Africa during from June 1966 to March 1967. During the course, 17, 097 mosquitoes belonging to 16 species and 224 bats belonging to 3 species were collected and processed for arbovirus content. An agent isolated from Culex decens was identified to be a strain of H 336 virus (Table 1, 3, 5, 7, 8). Twelve arboviruses were also isolated from the salivary glands of bats (Talbe 2). Two of 10 strains from Tadarida pumila were identified to be Bukalasa Bat virus (Table 4, 6, 7, 9). Another strains including 2 strains from Tadarida condylura await identification. Seventy-eight sera of human beings residing in Mwanza were examined for HI antibodies against 8 different antigens (3 Group A, 4 Group B and Bunyamwera). The incidence was highest with Chikungunya virus (73%), which was thought to be due to the epidemic of O\u27nyong-nyong fever in East Africa occurred in 1959. On the other hand, low incidence of Yellow fever antibodies (9%) was also pointed out (Table 10). One hundred and eighty-two materials, chiefly throat swab, were collected from the patients with fever. These materials were processed for virus isolation by using HeLa cells culture and 20 strains of viruses were isolated up to the present. Identification studies of these viruses are being carried out (Table 11, 12). We wish to express our sincere thanks to all staffs of East African Institute for Medical Research, Mwanza for their kindness to accomplishment of our survey, and also express our thanks to Dr. G. W. Kafuko, Dr. M. C. Williams and Dr. B. E. Henderson of East African Virus Research Institute, Entebbe, Uganda for their kind help of identification studies of isolated arboviruses. And also, we wish to acknowlege the kind identification of bats by Dr. F. A. Mutere, Makerere University College, Kampala, Uganda

保育（施設）実習指導の一環としてのボランティア活動の実際～全国保育士養成協議会東北ブロック内における調査より（2）～

departmental bulletin pape

Ecological Studies on Japanese Encephalitis Virus. Isolation of Japanese Encephalitis Virus from Mosquitoes Collected in Nagasaki and Kagoshima districts, Japan, in 1965.

Continual survey in Nagasaki prefecture were established at Kuromaru village since last year. In this season, 13 places in Nagasaki prefecture and the suburbs of Taniyama city, Kagoshima prefecture, were selected additionally for the collection of mosqui