Estimation of impact of remodeling and microbial factors on the results of total endoprosthesis of a knee joint in patients, suffering rheumatoid arthritis

Objective. To estimate the impact of remodelling and microbial factors on the results of total endoprosthesis (ТЕP) of the knee joint in patients, suffering rheumatoid arthritis (RА). Маterials and methods. The results of microbiological investigations of operative specimen were compared with results of cloning of the stem stromal cells of the bone marrow. Міcrobiologically and microscopically there were studied 247 specimen of operative material, obtained during performance of primary TЕP of a knee joint in 64 patients, suffering RA. Results. As the investigations have shown, in 1 сm³ of spongious bone of distal femur there is in 1.4 times more a general quantity of cells, containing the nuclei, than in 1 сm3 of spongious bone of proximal tibiae (the difference is not statistically significant). In distal part of femur the quantity of a colony-creating units of fibroblasts (CCUF) in 1 сm³ is in a 9.8 times lower, than the CCUF quantity in 1 сm³ in proximal part of tibiae (the difference is statistically significant), аnd the efficacy of the CCUF cloning is accordingly more than in 2.1 times lower (difference is statistically nonsignificant). Conclusion. High level of contamination of predominantly gram-positive microorganisms of the bone operative material, obtained while primary TEP of a knee joint in 82.8% patients, suffering RA, have a strong (almost a functional one) linear adverse correlation (coefficient r = -0.98) with osteogenic аctivity of the stem stromal cells in the bone marrow, which form a knee joint, what necessitates overestimation of approaches, concerning preparation and performance of surgical intervention for guaranteeing the efficacy of orthopedic treatment and standards of the protocols

Diagnostic value of <i>Rf1</i> gene molecular markers in sunflower

Background. Modern production of sunflower seeds is currently based on the cultivation of high-yielding heterotic F1 hybrids from crossbreeding of lines with cytoplasmic male sterility (CMS) of PET1-type and fertility restorer lines. The paternal parent serves as a donor of the nuclear Rf1 gene functional allele, which is responsible for pollen fertility restoration in F1 plants. The detection of carriers of the Rf1 locus recessive and dominant alleles using diagnostic molecular markers accelerates breeding of female and male parental lines for creating hybrids.Materials and methods. The material for the study included 75 lines of various origins from the VIR sunflower genetic collection as well as hybrids from crosses of VIR 116A sterile line with fertile lines differing in the type of cytoplasm (fertile or sterile) and the presence of molecular markers, most of which were linked to the Rf1 locus. For marker validation, two different approaches were used: either by analyzing associations between the ability of a line to restore pollen fertility and the presence of molecular markers in its genotype, or by estimating recombination frequency between the Rf1 locus and marker loci in four segregating hybrid populations.Results. According to the obtained results, no markers demonstrated 100% efficiency in the analysis of the sample of genotypes. The ORS511 marker was most frequently observed among the lines presumably carrying the dominant allele Rf1. Pollen fertility of F1 hybrids from interline crossings was 89-99%. The segregation for fertility/sterility in F2 fitted the theoretical ratio of 3:1 expected in case of the monogenic control of the trait. The markers HRG01, HRG02 and ORS511 were linked to the fertility restoration trait, with recombination rates between Rf1 locus and markers varying in different cross combinations. The analysis of VIR 116А × VIR 740 and VIR 116А × RIL 130 hybrids showed that among the marker loci studied, the ORS511 was closest to the Rf1 locus Rf1 (recombination frequency of 2.2 and 3.3%, respectively). The recombination rate between the Rf1 and ORS511 loci equaled 7.5% in the cross VIR 116А × VIR 210 and 8.9% in VIR 116 × VIR 195.Conclusion. The markers ORS511, HRG01 and HRG02 are the most efficient for the identification of alleles of the Rf1 gene and for the marker assisted selection in hybrid populations produced involving sunflower lines from the VIR collection

PATHOMORPHOLOGICAL FEATURES OF BONE LESIONS AND CORRELATION OF CLINICAL, LABORATORY AND MORPHOMETRIC CRITERIA IN PATIENTS WITH LATENT SCLEROSING HEMATOGENOUS OSTEOMYELITIS (GARRÉ)

Introduction. Sclerosing hematogenous osteomyelitis of Garré (SHO) holds a significant place among cases of latent hematogenous osteomyelitis. Pathomorphological studies of sclerosing hematogenous osteomyelitis are needed to improve differentiated diagnostics, to prognosticate morphology specifics of nidus and to choose the optimal treatment tactics.Purpose of the study — to identify statistical differences between manifestation patterns of various disease types and correlation between clinical, laboratorial and morphometric criteria of bone lesions in patients with sclerosing hematogenous osteomyelitis.Material and methods. The authors studied tissue fragments of affected bones of 25 patients with SHO which was diagnosed by clinical, laboratorial, visualizing and morphological methods. Gradation morphometric criteria were used to reflect condition of nidus tissues. The authors made the analysis of qualitative characters and correlation analysis of dependencies between clinical and laboratorial criteria on the one hand, and with morphometric criteria — on the other, and identified association factor. Results. Pathomorphological study of SHO nidus demonstrated variances of bone lesions in separate disease cases despite the overall similarity of morphological manifestation. About 2/3 of all cases correspond to fibrosing type whereby even small exudative inflammation sites are absent. In about 1/3 of all cases apart from fibrosis, osteosclerosis and remodeling, the osteomyelitis niduses contain microabscesses indicative of suppurative inflammation as well as the authors observed small sequestration. The maximum differences in patients with various types of SHO were identified in such parameters as share of stab microphages and erythrocyte sedimentation rate (ESR). In cases of long term morbidity the fibrosing disease type is prevailing, in cases of short term lesion (1–2 years) — a fibrosing type with microabscesses formation.A series of correlation dependencies was reported between clinical and laboratorial criteria on the one hand, and with morphometric criteria — on the other hand.Conclusion. Pathomorphological features of bone lesions in sclerosing hematogenous osteomyelitis are nonhomogeneous. Every case in the study demonstrated stereotype alterations such as substantial diffuse fibroses of marrow spaces in cancellous bone and bone regenerate, remodeling of bone tissues with gradual acceleration of osteosclerosis and persisting productive inflammation. Only in sone cases featured small sites of exudative inflammation — microabscesses with microsequestration