Sexual dimorphism of the human corpus callosum: Digital morphometric study

Background/Aim. Changes in the morphology and the size of the corpus callosum, are related to various pathological conditions. An analysis of these changes requires data about sexual dimorphism of the corpus callosum, which we tried to obtain in our study. We also investigated the method of digital morphometry and compared the obtained results with the results of other authors obtained by magnetic resonance imaging or by planimetry. Methods. A morphological research included 34 human brains (cadavers of both sexes − 19 female and 15 male aged 26−72 years). By digital morphometry using an AutoCAD software we performed measurements in the corpus callosum: the length (L), width in the half of its length (WW’), length of its cortical margin (LCM), area and perimeter of the anterior and posterior callosal segments, as well as the area and perimeter of the corpus callosum section area. The investigated parameters were analyzed and compared between the females and males. Results. There was not a statistically significant difference between the males and females in the investigated parameters of the corpus callosum (t test; p > 0.05), including the mean values of the two most important parameters, the surface of its midsagittal section area (males 654.11 mm2; females 677.40 mm2) and of its perimeter (males 19.61 cm; females 19.72 cm). The results obtained by digital morphometry were in the range of the results of other authors obtained by magnetic resonance and by planimetry. However, the value of Pearson coefficient of linear correlation between the section surface area and perimeter of the corpus callosum in the males was highly significant (rxy = 0.6943, p < 0.01), while in the females this value was statistically insignificant. Conclusion. Digital morphometry is accurate method in encephalometric investigations. Our results suggest that the problem of sexual dimorphism of the corpus callosum is very complex, because the identical variables (section surface area or its perimeter) do not exhibit the same behavior in males and in females, implicating that these variables even cannot be simply compared between the sexes

Importance of inflammation in arteriosclerotic plaque destabilization and rupture

Introduction. Although Rudolf Virchow considered arteriosclerosis an inflammatory disease in his book Cellular Pathology published in 1858, the opinion that it was a degenerative arterial disease as a civilization disease prevailed. Nowadays, a great attention has been paid to the inflammatory process in the pathogenesis of arteriosclerosis and particularly in the destabilization and rupture of plaque. Aim. To find out whether T and B lymphocytes, lipid macrophages, vascular smooth muscle and mast cells as well as plaque destabilization and rupture are present in ruptured arteriosclerotic plaque in the coronary arteries. Methods. Histochemical and immunochemical analyses of 68 ruptured arteriosclerotic plaques from the coronary arteries were performed. Microscopic examination revealed the presence of inflammation elements in all of them. The following histochemical and immunochemical methods were applied: Masson's trichrome, actins, vimentin, CD3, CD43, CD68, CD20, CD45 and chlorine acetyl esterase. The control group included 10 arteriosclerotic plaques from the coronary arteries with fibrous cap, but without inflammation cells. Results. Rupture of the arteriosclerotic plaque fibrous cap, with thinned and torn collagen fibers, was found in all of the 68 arteriosclerotic plaques. In 57 out of 68 analysed plaques, the increased number of T-lymphocytes, lipid macrophages, vascular smooth muscle and mast cells particularly on the plaque rupture site were found. In the remaining 11 specimens, mast cells were present in a somewhat smaller number. In the control group with the stable plaque, inflammation cells were not observed. Conclusion. Our results pointed out that the inflammatory elements, which might exert an effect upon the arteriosclerotic plaque destabilization, and rupture had been present in the ruptured arteriosclerotic plaque

Morphological changes in aorto-coronary vein graft: The analysis of autopsy and biopsy material

Background. Patients with implanted aortic coronary grafts have different survival time, which raises the question why the efficacy of graft implants is so poor. The aim of this study was to present the results of the analysis of morphological changes in the vein grafts taken after the death of patients who died after surgery in different time intervals, as well to present the analysis of the grafts obtained after surgical reintervention. Methods. The total number of 656 grafts of 308 dead patients was analyzed, as well as 76 grafts from 40 patients who underwent surgical reintervention. According to the duration of the graft since surgical intervention until death, all the analyzed changes were divided into two groups: a) early changes and complications, and b) late changes and complications in aorto-coronary vein grafts. Results. After the autopsy, 518 vein grafts from the first group were evaluated histopathologically. Changes were found in the form of small or large areas with peeled endothelium in 266 grafts, with the insudation of fibrin and thrombocytes in such places, subendothelial edema, and occlusive thrombosis of the graft lumen. Significant stenosis, which occurred distally from the anastomoses, was present in 118 grafts without changes in the walls of the graft, and there was significant narrowing of the graft lumen in 134 vein grafts due to intimal hyperplasia. In the second group, 138 grafts were histopathologically analyzed after autopsy. Significant hyperplasia was present in 117 grafts with the migration of smooth muscle cells from media into intima, and in 21 grafts there were atheromatous plaques. In 120 veins analyzed before the graft implantation, the lesion or the lack of endothelium was found, as well as the penetration of fibrin and blood elements and intimal hyperplasia. In 46 veins analyzed before the graft implantation, significant intimal hyperplasia with the elevated number of smooth muscle cells was found. Conclusion. The most frequent lesions in the grafts were the lesions of the endothelium, which caused thrombosis formation and lumen occlusion. Intimal hyperplasia in patients with longer survival time occurred due to the migration of smooth muscle cells from the media, or due to the formation of atherosclerotic plaques, which caused graft lumen stenosis or thrombosis

Migration influence on distribution of AB0 and Rh blood types in the area of Republic of Srpska

Migrations cause the gene flow in populations and represent the factor that changes its genetic structure. At the end of the previous century there was a significant migration and mixing of distant populations in the area of Republic of Srpska. Such changes inevitably cause the change of gene frequencies. The aim of our research is to analyze the distribution of phenotipic characteristics and to determine allele frequency for AB0 and Rh blood types in the mentioned area, and to compare the results with the previous research. The results were obtained from the Institute of Transfusion Medicine for the regions of Banja Luka, Prijedor, Gradiška, Novi Grad, Doboj and Trebinje. The results, displayed as tables and graphs, were analyzed by standard statistical methods, and they imply certain changes in allele frequencies that are not statistically significant

Elements of characterology in folklore music of Dinaric area

Dinaric type of man, with all its anthropological, genetic and psychological characteristics presents an orthodox example of patriarchal upbringing and tradition. Regardless of their patriarchalism and apparent insensitivity to other people, in almost every element of their intellectual work (music, dance, sazings, etc.) the fleshly and instinctive, that had to be satisfied regardless of all bans and restraints, and the message doubtless confirms that he did live in accordance with instincts, but at the same time he had to respect criteria of patriarchal moral. In this work the autors cite several songs from this area and analyze it from the perspective of psychology and characterology, finding the elements of love joy and sorrow, cure, passion, women shyness, etc

The research of morphological variations and sexual dimorphism of primary grooves on the medial side of brain hemispheres in humans

Morphological studies of the various parts of the brain show certain morphological and morphometric differences in correlation with sex, so-called sexual dimorphism of the brain. Our research has been done on the cerebral hemispheres, taken from cadavers of both sexes and different age without pathological processes in the brain. The sample comprised 26 male brains and 16 female brains. We studied three primary grooves (sulcus cinguli, sulcus parietooccipitalis and sulcus calcarinus) of the medial surface of the human cerebral hemispheres. We conducted morphological typology of grooves and morphometric measurements of primary brain grooves length in relation to sex and side of hemisphere. The results showed a statistically significant sex difference in the cingulate sulcus length (p0,05). Determined morphometric sexual dimorphism in cingulate sulcus length is significant because it implies the correlation between morphology and function of the explored areas of the cerebral cortex

Asymmetry and sexual dimorphism of the medial frontal gyrus visible surface in humans

Background/Aim. Studies of visible (extrasulcal) surface of the brain hemispheres are not feasible for measurements of the brain size, but are valuable for analysis and quantification of sexual dimorphism and/or asymmetries of the human brain. Morphological and morphometric investigations of the brain may contribute in genetic studies of the human nervous system. The aim of this study was to determine and to quantify sexual dimorphism and the right/left morphological asymmetry of the visible surface of medial frontal gyrus (gyrus frontalis medialis - GFM). Methods. Measurements and analysis of the visible surface of GFM were done on 84 hemispheres (42 brains from the persons of both sexes: 26 males and 16 females, 20-65 years of age). After fixation in 10% formalin and dissection, digital morphometric measurements were performed. We studied these in relation to the side of the hemisphere and the person's sex. Standardized digital AutoCAD planimetry of the visible surface of GFM was enabled by the use of coordinate system of intercommissural line. Results. In the whole sample, the visible surface of the right GFM (21.39 cm2) was statistically significantly greater (p < 0.05) than the left GFM (18.35 cm2) indicating the right/left asymmetry of the visible surface of GFM. Also, the visible surface of the right GFM in the males (22.66 cm2) was significantly greater (p < 0.05) than in the females (19.35 cm2), while the difference in size of the left GFM between the males and the females was not significant (p > 0.05). Conclusion. Morphological analysis of visible surface of GFM performed by digital planimetry showed sexual dimorphism of the visible surface and the presence of right/left asymmetry of GFM

Quantitative defining of acetabulum using 3D-CAD model of hip joint

Finding a real acetabulum position is an important clinical and biomechanical problem. Knowing the exact linear and angle dimensions is of vital importance for the success of numerous clinical and biomechanical procedures. 3D hip joint model was developed in this study in an original way on the basis of CT data. We used the potentials of CAD software CA TIA V-5 and its modules. We showed multiple clinical applications of this method, such as: Pre-operative planning in hip arthroplasty, making individualized implants and biomechanical measurements. Also, we compared this method to other similar methods of visualization and 3D reconstruction in medicine. Our parameter-defined 3D acetabulum model is characterized by complete (dimensionism) and photorealistic presentation. Other programs have only one or other previously mentioned features.. We showed the possibility for improving the perfection of presented CAD method and we proposed the methods of how to achieve the same

Comparative analysis of anthropometric parameters of the primary school pupils of urban and rural Doboj region

An investigation was conducted on the sample of primary school students in urban area with the aim of analyzing the anthropometric parameters between boys and girls entering 1st-9th grade. The main objective is to determine the frequency and causes of obesity in the population of school children with the intention of preventing the risks of developing the 'modern man' diseases (diabetes, hypertension, hypercholesterolemia, the development of metabolic syndromes, coronary heart disease, etc). The basic anthropometric parameters were measured: body weight and height, chest, waist and hips circumference, and from the obtained values the following were calculated: body mass index, ratio of waist circumference to body height and ratio of waist circumference to hips circumference. The comparison of the results according to age and sex of the pupils is expected to give the values that might answer some questions, such as: the degree of development and nutritional status of schoolchildren according to age and sex in relation to the environment in which they live, the annual increment of the measured parameters value, the differences between age and gender groups. Results were compared with parameters of growth and development of the children of same age in rural areas based on earlier research. The goal of the study is to confirm or reject the existence of a linear regression in correlation to some anthropometric parameters in relation to sex and age of the pupils from urban and rural areas, by means of the allometric method

Sexual dimorphism of hyoid bone

Hyoid bone, is located in anteriorsuperior site of the neck. Represents the odd bone which is the only bone in the human body that is not articulated or otherwise normally, associated with any other bone. Bearing in mind its embryonic origin, the bone is part of the skeleton viscerocranium. It has a horseshoe shape and consists of a body, two large and two small horns. In our study, we investigated sexual dimorphism hyoid bone. Testing we did at 42 hyoid human cadaver bones with known sex and age. We made 5 oseometric mesaurements. Metric analysis of the hyoid bone is a useful technique in the determination of sexual dimorphism hyoid bone that can give good results, but is not entirely satisfactory, and can serve as a complementary method