The Effects of Different Diet Models on Oocyte Quality, Oocyte Maturation and in Vitro Fertilization Outcomes in Mice

Nutritional quality affects fertility in both men and women. Nutrition should be considered in the prevention and treatment of infertility in men and women. In this study, we evaluated IVF rates, oocyte maturation and oocyte quality in a female animal model feding ketogenic, western and standard type diet. Female BALB / c mice were allocated to three groups: (a) control; (b) Western and (c) Ketogenic. After feeding female mice with these 3 feeding groups for 4 weeks, IVF was administered before sacrification of the mice. The number of collected oocytes and oocyte quality, fertilization rates and embryo quality were evaluated.The number of total oocytes was the highest in the ketogenic diet and the least in the western diet. MII stage oocytes in the ketogenic group were statistically significantly higher than the control group and western groups. Fertilized oocyte count was significantly lower in the western group compared to the other two groups. When the two-cell zygote stage was evaluated, it was found that the results were better in the ketogenic diet group and worse in the western diet group. The worst results in terms of the number of zygotes reaching the blastocyst stage were observed in the western type nutrition group, and the best results were observed in the group on the keteogenic diet. The results of our study show that western nutritional models negatively affect IVF results, while ketogenic diet has a positive effect. Keywords: Fertility, ketogenic diet, oocyte, ovulation, western diet DOI: 10.7176/JMPB/72-02 Publication date: January 31st 202