1 research outputs found
Optimization of the medium composition for production of antimicrobial substances by bacillus subtilis ATCC 6633
In the effort to overcome the increase in antimicrobial resistance of
different pathogens, natural products from microbial sources appear to be the
most favorable alternative to current antibiotics. Production of
antimicrobial compounds is highly dependent on the nutritional conditions.
Hence, in order to achieve high product yields, selection of the media
constituents and optimization of their concentrations are required. In this
research, the possibility of antimicrobial substances production using
Bacillus subtilis ATCC 6633 was investigated. Also, optimization of the
cultivation medium composition in terms of contents of glycerol, sodium
nitrite and phosphates was done. Response surface methodology and the method
of desirability function were applied for determination of optimal values of
the examined factors. The developed model predicts that the maximum
inhibition zone diameters for Bacillus cereus ATCC 10876 (33.50 mm) and
Pseudomonas aeruginosa ATCC 27853 (12.00 mm) are achieved when the initial
contents of glycerol, sodium nitrite and phosphates were 43.72 g/L, 1.93 g/L
and 5.64 g/L, respectively. The results of these experiments suggest that
further research should include the utilization of crude glycerol as a carbon
source and optimization of composition of such media and cultivation
conditions in order to improve production of antimicrobial substances using
Bacillus subtilis ATCC 6633