Promene u stepenu galaktozilacije IgG kunića tokom dugotrajne imunizacije goveđim serumskim albuminima

The galactose content of isolated IgG was analyzed before immunization and at intervals during prolonged immunization over 16 months. It was found that IgGs from non-immunized and immunized animals differed in galactose content and that the galactose content of serum IgG varied during the course of the immune response. These results suggest possible intraclonal variations or the emergence of subclones of cells secreting IgG that is differently galactosylated relative to the IgG of non-immunized animals. Since isotypic polymorphism of IgG (subclasses), which determines the effector potential(s) of IgG antibodies, does not exist in rabbits, glycosylation may be what defines functional subsets of isotypically uniform antibodies produced during an immune response in these animals.Ugljeni hidrati su strukturni element imunoglobulina G (IgG), bitan za konformaciju i efektorne funkcije molekula. Ugljenohidratni sadržaj čine oligosaharidni lanci (glikani) vezani kovalentno za konzervirani Asn 297 u svakom CH2 domenu Fc regiona IgG molekula. Sadržaj pojedinih šećera koji ulaze u sastav glikana nije isti u svim molekulima IgG. Imunoglobulini G su populacija različitih glikoformi čiji sadržaj varira pod fiziološkim, a naročito pod raznim patološkim uslovima. Pretpostavlja se da su te varijacije odraz promena u distribuciji specifičnih antitela IgG klase tokom tekućeg često hroničnog, imunog odgovora na neki antigen. O dinamici tih promena se međutim, malo zna, a posebno o pojavi glikoformi tokom imunog odgovora koje bi mogle imati patogenetski značaj. U ovom radu su analizirane promene u glikozilaciji serumskih IgG tokom imunog odgovora kunića dugotrajno imunizovanih goveđim serumskim albuminima. Rezultati su ukazali da je taj odgovor praćen permanentnim promenama u sadržaju terminalne galaktoze. S obzirom da su molekuli IgG kunića, za razliku od drugih sisara, izotipski uniformni, tj. nemaju podklase (kojima je determinisana brojnost efektornih funkcija IgG), razlike u galaktozilaciji bi mogle determinisati pojavu funkcionalnih subsetova IgG antitela stvorenih tokom imunog odgovora kod ove vrste

Engineering Water- and Land-Scapes: Swimming Pools Design Guidelines and Methodologies between the Freeform Leisure and Professional Water Sports Standards [Recognition] [STRAND 2021]

ON ARCHITECTURE 2021, FACING THE FUTURE – NEW CHALLENGES, 9th International Conference and Exhibition, Serbian Academy of Sciences and Arts, Gallery of Science and Technology, Belgrade, SerbiaJURY REPORT International Jury Members in alphabetical order: Zoran Abadić (Serbia), Stahl Stenslie (Norway), Venetia Tsakalidou (Greece) and Renate Weissenböck (Austria).Recognition ENGINEERING WATER- AND LAND-SCAPES: Swimming Pools Design Guidelines and Methodologies Between the Freeform Leisure and Professional Water Sports Standards By Dragana Ćirić (Serbia) CATEGORY: ARCHITECTURE AND ART – Architectural Designs: Urban DesignThis project shows us how aesthetics, design, public programme and land formation can blend together beautifully into one architectural gesture. It suggests an innovative take on architecture of a larger scale and urban design that is worthy of a recognition. It proposes a unified complex consisting of different water environments and spaces, which integrate smoothly with their surroundings, the waterfront and the river basin, as if they were always there in the first place. With this project, architecture and landscape are brought closer to the community in order to improve the quality of life of the inhabitants and offer a variety of water sports and activities in a well-articulated mechanism.Rad se nalazi u delu publikacije - Nasl. str. prištampanog teksta: Exhibition boo

Diagramma / Datascapes: Metacognitive Strategies [Recognition] [STRAND 2017]

The Fifth International Exhibition ON ARCHITECTURE 2017 Jury (A-Z): Elif Ayiter, Mitesh Dixit, Stanko Gaković, Nora Lefa, Cristian Suau, Anastasios Tellios, Wilfried van WindenCategory INNOVATION IN ARCHITECTURE: Diagramma / Datascapes: Metacognitive Strategies by Dragana Ćirić awarded with RECOGNITIONThe project addresses the act of generating diagrams used in the procedure of architecture. It captivates the spirit of complexity of a robust scientific, analytic tool, while at the same time it breathes the air of an innovative, aesthetically pleasant visual composition. The monochromatic design attributes enhance and strengthen its distinct characterhttps://strand.rs/2017/architecture/exhibition/competition-results/https://dciricdiagramma.tumblr.com/https://dciricmicrohistoriesdiaseries02.tumblr.com/https://vimeo.com/438134603Rad se nalazi u delu publikacije - Nasl. str. prištampanog teksta: Exhibition Boo

Digital Spatial Diagrammatics: Space and Information in the Age of Strategic Design Intelligence [datascapes]

The core interest of this study will be the relationship involving space, data/information, spatial inscription and representation, reasoning, and decision-making as part of the current digital phase. We will look at how digitality (as the cultural and philosophical framework), digital sciences and technologies (as its practical counterpart), and digital media and creativity have transformed spatial awareness, action, notation, and imagery, and what role spatial and architectural design thinking and performance have taken within these tendencies as both their initiators or leading developers and their contributors, highly influenced by digital technologies, tools, and the unlimited design possibilities warranted in the digital world.Starting from the outline of space informatisation and cyberisation as a broader social condition, a more focused argument will be built upon the question of what digital diagrammatics stands for. Operating along the scale from the logical abstractions and complexities in inferencing that guide intellectual plots, processing, and relational networking behind the ideas and concepts to their materiality. objecthood, or different physical manifestations, digital diagrammatics has acquired a specific role of spatial agency through data. Datascapes—environments defined, designed, or mapped by data; scapes of information; superpositions of multiple physical and virtual realities inasmuch as one of them alone—will be examined from the diagrammatic architectural perspective, asking: How do diagrammatic and spatial strategies employ and articulate data for different purposes, and what are the outcomes of digital data operations in a diagrammatic spatial layout? What are the ways in which they are practiced across the disciplinary system while dealing with data in a documentary, scientific, or creative manner? Supported by comments on historical precedents and forms that laid their foundations, the answers on how digital language and literacy (as pivotal features of the underlying system of new digital media and its hybrid aesthetics) have transformed the ways in which spatial or any other information is used and comprehended are expected to come as a conclusion.Invited Participation (by Prof. Amirouche Moktefi); Tallinn University of Technology (TalTech), Tallinn, Estonia; Department of Law, TalTech; Ragnar Nurkse School of Innovation and Governance, TalTech; TalTech Mektory, L’Institut Françaisd’Estonie (IFE) ; Organisers: Peeter Müürsepp (Department of Law, TalTech), Ahti-Veikko Pietarinen, Amirouche Moktefi, Marika Proover (Ragnar Nurkse School of Innovation and Governance, TalTech) ; Session Chair: Raul Veed

The Science of Networks: Grand Paris

The science of networks can be considered a key instrument for complex systems analysis or analytics, design, and planning. Under its framework and through its main analytical methods and forms (network graphs and related operations), one of the urban subsystems—urban mobility and transportation system—has been represented and investigated. The Grand Paris rail network, including all its transportation categories with their existing lines and extensions currently either in construction and planned or under consideration in the long term, has been modelled as a background topological and simulation environment and geometry for various abstract and real-world urban computing research operations and generative design tasks (e.g., urban movement path generation, the network’s incremental growth and reconfiguration, spatial urban scenarios constructions, etc.).Related Works: Invitation for OA2023 Conference Section (by Prof. Ružica Bogdanović

Architectural Scenery and Natural Light Dynamics

The study analyses two design issues in relation to formal and programmatic architectural design aspects and decision-making: 1. natural light dynamics within the broader framework of architectural articulation of environmental properties, and 2. architectural scenery, supported by the cinemetric graphic research guidelines and methods. Parameters of architectural atmosphere directed by the environmental conditions have been converged with the cinemetric reasoning and representation in order to broaden the scopes of each of the stated architectural design research methods, concerns, and constitutive elements of various design strategies. Both themes have been complemented with the methodology of design through 3D modelling and visualisation, using such mediums as corrective instruments and design control mechanisms indicating consistencies or inconsistencies of the proposed scientific design claims.Two architectural projects for private houses have been re-deployed for demonstration of the investigative process and methodology due to their light- and environment-infused design determinants and scenery. The argued interior mechanisms and concepts of contingency between the daily time-dynamics and thus regulated programmatic activities inside the architectural space have been targeted, resulting in sequences of sunlight- and skylight-built atmospheres with the diagrammatic explanation of instances that have directed such results.Invited Conference Contribution (by Professor Ružica Bogdanović

Čumi, Bernar [Bernard Tschumi]. Arhitektonski paradoks

Prevod teksta Arhitektonski paradoks od Bernara Čumija [Bernard Tschumi

Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display (vol 7, 429, 2016)

Microbial surface and secreted proteins (the secretome) contain a large number of proteins that interact with other microbes, host and/or environment. These proteins are exported by the coordinated activities of the protein secretion machinery present in the cell. A group of bacteriophage, called filamentous phage, have the ability to hijack bacterial protein secretion machinery in order to amplify and assemble via a secretion-like process. This ability has been harnessed in the use of filamentous phage of Escherichia coli in biotechnology applications, including screening large libraries of variants for binding to “bait” of interest, from tissues in vivo to pure proteins or even inorganic substrates. In this review we discuss the roles of secretome proteins in pathogenic and non-pathogenic bacteria and corresponding secretion pathways. We describe the basics of phage display technology and its variants applied to discovery of bacterial proteins that are implicated in colonization of host tissues and pathogenesis, as well as vaccine candidates through filamentous phage display library screening. Secretome selection aided by next-generation sequence analysis was successfully applied for selective display of the secretome at a microbial community scale, the latter revealing the richness of secretome functions of interest and surprising versatility in filamentous phage display of secretome proteins from large number of Gram-negative as well as Gram-positive bacteria and archaea

Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display

Microbial surface and secreted proteins (the secretome) contain a large number of proteins that interact with other microbes, host and/or environment. These proteins are exported by the coordinated activities of the protein secretion machinery present in the cell. A group of bacteriophage, called filamentous phage, have the ability to hijack bacterial protein secretion machinery in order to amplify and assemble via a secretion-like process. This ability has been harnessed in the use of filamentous phage of Escherichia coli in biotechnology applications, including screening large libraries of variants for binding to "bait" of interest, from tissues in vivo to pure proteins or even inorganic substrates. In this review we discuss the roles of secretome proteins in pathogenic and non-pathogenic bacteria and corresponding secretion pathways. We describe the basics of phage display technology and its variants applied to discovery of bacterial proteins that are implicated in colonization of host tissues and pathogenesis, as well as vaccine candidates through filamentous phage display library screening. Secretome selection aided by next-generation sequence analysis was successfully applied for selective display of the secretome at a microbial community scale, the latter revealing the richness of secretome functions of interest and surprising versatility in filamentous phage display of secretome proteins from large number of Gram-negative as well as Gram-positive bacteria and archaea