Single-molecule analysis of 1D diffusion and transcription elongation of T7 RNA polymerase along individual stretched DNA molecules

Using total intrnal reflection fluorescence microscopy, we directly visualize in real-time, the 1D Brownian motion and transcription elongation of T7 RNA polymerase along aligned DNA molecules bound to substrates by molecular combing. We fluorescently label T7 RNA polymerase with antibodies and use flow to convect them orthogonally to the DNA alignment direction, permitting observation and estimation of the protein diffusivity along the DNA at the single-molecule level. Our observations suggest that the 1D diffusion coefficient varies from molecule to molecule over the range 6.1 × 10−11 cm2/s to 4.3 × 10−9 cm2/s. We also observe binding and transcription by T7 RNA polymerases on single combed T7 DNA molecules with an apparent association rate of 1.6 μM−1s−1. From the measured dependence of the rate of transcription on concentration of nucleotide triphosphate, we infer that the combed DNA molecules capable of interacting with proteins are under an average tension of 25 pN

High Risks of Losing Genetic Diversity in an Endemic Mauritian Gecko: Implications for Conservation

Genetic structure can be a consequence of recent population fragmentation and isolation, or a remnant of historical localised adaptation. This poses a challenge for conservationists since misinterpreting patterns of genetic structure may lead to inappropriate management. Of 17 species of reptile originally found in Mauritius, only five survive on the main island. One of these, Phelsuma guimbeaui (lowland forest day gecko), is now restricted to 30 small isolated subpopulations following severe forest fragmentation and isolation due to human colonisation. We used 20 microsatellites in ten subpopulations and two mitochondrial DNA (mtDNA) markers in 13 subpopulations to: (i) assess genetic diversity, population structure and genetic differentiation of subpopulations; (ii) estimate effective population sizes and migration rates of subpopulations; and (iii) examine the phylogenetic relationships of haplotypes found in different subpopulations. Microsatellite data revealed significant population structure with high levels of genetic diversity and isolation by distance, substantial genetic differentiation and no migration between most subpopulations. MtDNA, however, showed no evidence of population structure, indicating that there was once a genetically panmictic population. Effective population sizes of ten subpopulations, based on microsatellite markers, were small, ranging from 44 to 167. Simulations suggested that the chance of survival and allelic diversity of some subpopulations will decrease dramatically over the next 50 years if no migration occurs. Our DNA-based evidence reveals an urgent need for a management plan for the conservation of P. guimbeaui. We identified 18 threatened and 12 viable subpopulations and discuss a range of management options that include translocation of threatened subpopulations to retain maximum allelic diversity, and habitat restoration and assisted migration to decrease genetic erosion and inbreeding for the viable subpopulations

Changes in Atmospheric Dynamics Over Dansgaard‐Oeschger Climate Oscillations Around 40 ka and Their Impact on Europe

AbstractDansgaard‐Oeschger (D‐O) climate variability during the last glaciation was first evidenced in ice cores and marine sediments, and is also recorded in various terrestrial paleoclimate archives in Europe. The relative synchronicity across Greenland, the North Atlantic and Europe implies a tight and fast coupling between those regions, most probably effectuated by an atmospheric transmission mechanism. In this study, we investigated the atmospheric changes during Greenland interstadial (GI) and stadial (GS) phases based on regional climate model simulations using two specific periods, GI‐10 and GS‐9 both around 40 ka, as boundary conditions. Our simulations accurately capture the changes in temperature and precipitation as reconstructed by the available proxy data. Moreover, the simulations depict an intensified and southward shifted eddy‐driven jet during the stadial period. Ultimately, this affects the near‐surface circulation toward more southwesterly and cyclonic flow in western Europe during the stadial period, explaining much of the seasonal climate variability recorded by the proxy data, including oxygen isotopes, at the considered proxy sites.Plain Language Summary: The climate during the last ice age varied between colder and warmer periods on timescales ranging from hundreds to thousands of years. This variability was first detected in Greenland ice cores and marine sediment cores of the North Atlantic, as well as in continental geological records in Europe. The variation between the colder and warmer periods occur mostly simultaneously in Greenland and in Europe, which is why the atmosphere is assumed to have an important role in transferring the climate signals. We simulated two different periods of the last ice age, one colder and one warmer around 40,000 years ago, using a regional climate model. The aim was to study how the climate and atmospheric circulation changed during these two periods. We find the eddy‐driven jet over the North Atlantic intensified and shifted southward during the colder period. The jet influences the near‐surface atmospheric circulation and leads to more southwesterly and cyclonic flow in western Europe. Oxygen isotope variations observed in western European paleoclimate records may be partly explained by different, more southern moisture sources on top of changes in seasonal temperatures.Key Points: Simulated temperatures agree with proxy data; precipitation is biased but GI‐10 versus GS‐9 differences are well captured The stadial winter jet stream is intensified and shifted southward, consistent with dominant southwesterly/cyclonic flow in western Europe Oxygen isotope signal changes at western European proxy sites may be explained not only by temperature but also by varying moisture sources NRDIOAXA Research Fund http://dx.doi.org/10.13039/501100001961https://doi.org/10.5065/1dfh-6p9

Optical Characterization of Different Thin Film Module Technologies

For a complete quality control of different thin film module technologies (a-Si, CdTe, and CIS) a combination of fast and nondestructive methods was investigated. Camera-based measurements, such as electroluminescence (EL), photoluminescence (PL), and infrared (IR) technologies, offer excellent possibilities for determining production failures or defects in solar modules which cannot be detected by means of standard power measurements. These types of optical measurement provide high resolution images with a two-dimensional distribution of the characteristic features of PV modules. This paper focuses on quality control and characterization using EL, PL, and IR imaging with conventional cameras and an alternative excitation source for the PL-setup