BpOmpW antigen stimulates the Necessary Protective T-Cell Responses Against Melioidosis.

Melioidosis is a potentially fatal bacterial disease caused by Burkholderia pseudomallei and is estimated to cause 89,000 deaths per year in endemic areas of Southeast Asia and Northern Australia. People with diabetes mellitus are most at risk of melioidosis, with a 12-fold increased susceptibility for severe disease. Interferon gamma (IFN-γ) responses from CD4 and CD8 T cells, but also from natural killer (NK) and natural killer T (NKT) cells, are necessary to eliminate the pathogen. We previously reported that immunization with B. pseudomallei OmpW (BpOmpW antigen) protected mice from lethal B. pseudomallei challenge for up to 81 days. Elucidating the immune correlates of protection of the protective BpOmpW vaccine is an essential step prior to clinical trials. Thus, we immunized either non-insulin-resistant C57BL/6J mice or an insulin-resistant C57BL/6J mouse model of type 2 diabetes (T2D) with a single dose of BpOmpW. BpOmpW induced strong antibody responses, stimulated effector CD4+ and CD8+ T cells and CD4+ CD25+ Foxp3+ regulatory T cells, and produced higher IFN-γ responses in CD4+, CD8+, NK, and NKT cells in non-insulin-resistant mice. The T-cell responses of insulin-resistant mice to BpOmpW were comparable to those of non-insulin-resistant mice. In addition, as a precursor to its evaluation in human studies, humanized HLA-DR and HLA-DQ (human leukocyte antigen DR and DQ isotypes, respectively) transgenic mice elicited IFN-γ recall responses in an enzyme-linked immune absorbent spot (ELISpot)-based study. Moreover, human donor peripheral blood mononuclear cells (PBMCs) exposed to BpOmpW for 7 days showed T-cell proliferation. Finally, plasma from melioidosis survivors with diabetes recognized our BpOmpW vaccine antigen. Overall, the range of approaches used strongly indicated that BpOmpW elicits the necessary immune responses to combat melioidosis and bring this vaccine closer to clinical trials

INSPEX: design and integration of a portable/wearable smart spatial exploration system

International audienceThe INSPEX H2020 project main objective is to integrate automotive-equivalent spatial exploration and obstacle detection functionalities into a portable/ wearable multi-sensor, miniaturised, low power device. The INSPEX system will be used for 3D real-time detection, location and warning of obstacles under all environmental conditions in indoor and outdoor environments with static and mobile obstacles. Potential applications range from safer human navigation in reduced visibility conditions , small robot/drone obstacle avoidance systems to navigation for the visually/mobility impaired, this latter being the primary use-case considered in the project

Academic Outcome, Anxiety and Attitudes in Early and Late Immersion in Ireland

Differences between early and late Irish-immersion secondary school students are examined, not only in terms of academic outcome and target language ability, but also in terms of attitudes to learning the target language. Participants included a gender-balanced group of 97 students in Irish-immersion in fourth year of secondary school (mean age 15.5 years). The students were categorised as either early immersion (had attended an Irish-medium primary school) or late immersion students (Irish as core subject only until secondary). Participants completed a C-test and a Student Questionnaire based on Gardner's (1985) Attitude and Motivational Test Battery (AMTB), which looked in particular at their class anxiety, motivation and parental support for learning Irish. State examination results (Junior Certificate) were also collected and compared for the early and late immersion students. No difference was found between the groups in terms of overall academic attainment in Mathematics and Irish scores in Junior Certificate results. However the late immersion students performed significantly less well than early immersion student on more subtle tests of Irish ability and scored significantly higher on classroom anxiety. The discussion considers these outcomes and suggestions are made for provision of a transitional programme for late immersion students to address differences in their language proficiency and anxiety levels.SB. 17/4/2013Problem with Abstract, a correct one would not copy accross. SB

Irish orthography: what do teachers and learners need to know about it, and why?

Irish has significant State support, but lacks a research base to support the teaching of Irish reading. Current approaches to teaching Irish reading are presented, and outcomes summarised. Issues of consistency and complexity in Irish orthography are discussed in light of an analysis of a corpus of early reader texts, and the formulation of rules for discriminating between words which are regular by letter-sound and grapheme-sound rules is outlined. While the most frequent words show a high level of regularity, underlying rules are very complex. The need to target decoding skills early is discussed. Recommendations regarding the teaching of aspects of Irish orthography are presented.Author has checked copyrightAM

BpOmpW antigen stimulates the necessary protective T-cell responses against melioidosis

Melioidosis is a potentially fatal bacterial disease caused by Burkholderia pseudomallei and is estimated to cause 89,000 deaths per year in endemic areas of Southeast Asia and Northern Australia. People with diabetes mellitus are most at risk of melioidosis, with a 12-fold increased susceptibility for severe disease. Interferon gamma (IFN-γ) responses from CD4 and CD8 T cells, but also from natural killer (NK) and natural killer T (NKT) cells, are necessary to eliminate the pathogen. We previously reported that immunization with B. pseudomallei OmpW (BpOmpW antigen) protected mice from lethal B. pseudomallei challenge for up to 81 days. Elucidating the immune correlates of protection of the protective BpOmpW vaccine is an essential step prior to clinical trials. Thus, we immunized either non-insulin-resistant C57BL/6J mice or an insulin-resistant C57BL/6J mouse model of type 2 diabetes (T2D) with a single dose of BpOmpW. BpOmpW induced strong antibody responses, stimulated effector CD4+ and CD8+ T cells and CD4+ CD25+ Foxp3+ regulatory T cells, and produced higher IFN-γ responses in CD4+, CD8+, NK, and NKT cells in non-insulin-resistant mice. The T-cell responses of insulin-resistant mice to BpOmpW were comparable to those of non-insulin-resistant mice. In addition, as a precursor to its evaluation in human studies, humanized HLA-DR and HLA-DQ (human leukocyte antigen DR and DQ isotypes, respectively) transgenic mice elicited IFN-γ recall responses in an enzyme-linked immune absorbent spot (ELISpot)-based study. Moreover, human donor peripheral blood mononuclear cells (PBMCs) exposed to BpOmpW for 7 days showed T-cell proliferation. Finally, plasma from melioidosis survivors with diabetes recognized our BpOmpW vaccine antigen. Overall, the range of approaches used strongly indicated that BpOmpW elicits the necessary immune responses to combat melioidosis and bring this vaccine closer to clinical trials