[[alternative]]The Study of camphor sulfonic acid derivatives metal complex selective binding affinity to DNA

[[abstract]]This study utilize the cleavage activity of phage φX174 as the model DNA system to investigate the selectivity and affinity for a series camphor sulfonic acid derivatives [Cpd1: (C20H28N2O4), Cpd2: (C26H38N2O4), and Cpd3: (C27H26N4O4)] when complex with various metal ion. The cleavage activity is as follow: Zn2+ > Cu2+ >> Mn2+ ~ V4+ when complex with above series of camphor sulfonic acid derivatives as reveled from agarose electrophoresis experiment. Whereas the chemical shifting experiment in 1D 1H NMR (One-dimensional 1H nuclear magnetic resonance spectroscopy) show the all metal and camphor sulfonic acid derivatives would bind together. Whereas for the randomized sequence oligonucleotide, the AT-rich DNA sequence give better selectivity as forming stable 1:1 ratio complex such as: [d(CGAAATTTGCG)]2 > [d(CAGCTTG)]2 > [d(CCGG)]2 when investigated with CD (Circular dichroism) experiment.

Immobilization of lead by application of zeolite: Leaching column and rhizobox incubation studies

Application of zeolite can reduce lead (Pb) mobility in soil. Leaching columns and rhizobox incubation experiments were carried out to investigate the leaching processes and rhizosphere behavior of Pb in a Pb-contaminated soil amended with zeolite. Zeolite addition reduced Pb release from the contaminated soil as well as increasing leachate pH and decreasing the bioavailable Pb concentration. Leachate pH was not significantly different among different zeolite dose treatments at the same measurement time. Leaching of Pb from the treated soil was lower than that from the untreated soil for the first nine weeks but the trend was reversed for the final weeks of the study. The Pb concentration in the leachate did not appear to be sensitive to changes in pH. It was concluded that when a relatively low amount of zeolite was added (<20 mg kg(-1)), the cation exchange capacity was the dominant factor for regulating Pb leaching behavior. The DTPA extractable Pb in the rhizosphere was similar to 15% higher than that in the close-root and bulk soil. In addition, the amounts of DTPA extractable Pb in the rhizosphere soil not treated with zeolite were 10% and 16% higher than in the rhizosphere soil with 10 and 20 g kg-1 zeolite addition, respectively. It could be concluded that zeolite addition inhibits uptake of Pb by affecting rhizospheric behavior. (C) 2013 Elsevier B.V. All rights reserved.Application of zeolite can reduce lead (Pb) mobility in soil. Leaching columns and rhizobox incubation experiments were carried out to investigate the leaching processes and rhizosphere behavior of Pb in a Pb-contaminated soil amended with zeolite. Zeolite addition reduced Pb release from the contaminated soil as well as increasing leachate pH and decreasing the bioavailable Pb concentration. Leachate pH was not significantly different among different zeolite dose treatments at the same measurement time. Leaching of Pb from the treated soil was lower than that from the untreated soil for the first nine weeks but the trend was reversed for the final weeks of the study. The Pb concentration in the leachate did not appear to be sensitive to changes in pH. It was concluded that when a relatively low amount of zeolite was added (<20 mg kg(-1)), the cation exchange capacity was the dominant factor for regulating Pb leaching behavior. The DTPA extractable Pb in the rhizosphere was similar to 15% higher than that in the close-root and bulk soil. In addition, the amounts of DTPA extractable Pb in the rhizosphere soil not treated with zeolite were 10% and 16% higher than in the rhizosphere soil with 10 and 20 g kg-1 zeolite addition, respectively. It could be concluded that zeolite addition inhibits uptake of Pb by affecting rhizospheric behavior. (C) 2013 Elsevier B.V. All rights reserved