[[alternative]]Age,Time & Different leading style:Influences on Children's Episodic Memory

[[abstract]]This study explored children's episodic memory. 43 7-year-olds and 43 5-year-olds took part in a contrived interaction with a "magician".After seeing a magic show,the children were interviewed immediately and assignedrandomly in three conditions:no leading(free recall), leading,and misleading. Older children recall more accurate information than younger children, and both group recall more accurate information after short delay than the long delay.No matter younger or older children, they had ability to provide accurate testi-mony.Leading question facilitated free recall after three days. Leading group were more accurate than no leading and misleading group in the misleading suggestive questions.

Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells

Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. (C) 2013 Elsevier Ltd. All rights reserved.Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. (C) 2013 Elsevier Ltd. All rights reserved

Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants

A series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding region (PBR), which is driven by positive Darwinian selection. The coexistence of two isoforms in MHC IA, IIA, and IIB alleles are shown: one full-length transcript and one novel splice variant. Despite lake of the external domains, these variants exhibit similar subcellular localization with the full-length transcripts. Moreover, the expression of MHC isoforms are up-regulated upon in vivo and in vitro stimulation with Andrias davidianus ranavirus (ADRV), suggesting their potential roles in the immune response. The results provide insights into understanding MHC variation and function in this ancient and endangered urodele amphibian. (C) 2013 Elsevier Ltd. All rights reserved.A series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding region (PBR), which is driven by positive Darwinian selection. The coexistence of two isoforms in MHC IA, IIA, and IIB alleles are shown: one full-length transcript and one novel splice variant. Despite lake of the external domains, these variants exhibit similar subcellular localization with the full-length transcripts. Moreover, the expression of MHC isoforms are up-regulated upon in vivo and in vitro stimulation with Andrias davidianus ranavirus (ADRV), suggesting their potential roles in the immune response. The results provide insights into understanding MHC variation and function in this ancient and endangered urodele amphibian. (C) 2013 Elsevier Ltd. All rights reserved

Landslides on the Loess Plateau of China: a latest statistics together with a close look

Landslide plays an important role in landscape evolution, delivers huge amounts of sediment to rivers and seriously affects the structure and function of ecosystems and society. Here, a statistical analysis together with a field investigation was carried out on the Loess Plateau of China to address the challenges. The study tracks landslide-related deaths and collects knowledge about this natural hazard. Since the 1980s, 53 fatal landslides have occurred, causing 717 deaths. As the most important trigger, rainfall induced 40% of the catastrophic landslides, while other factors, i.e., human activities, freeze-thaw and earthquake, accounted for 36, 23 and 1%, respectively. Furthermore, landslide frequency and death toll related to human activities were increasing as time went on. Landslide also plays an important role in sediment delivery, especially in areas with steep terrain. Sediment discharge from landslides accounts for a considerable proportion of the total soil loss in the upper and middle reaches of the Yellow River. In some catchments of the Loess Plateau, landslides contributed over 50% of the total sediment discharge. The result shows that landslide is a widespread geologic hazard in the rural area of the Loess Plateau, China