Allelopathic Observations in Rice (Oryza sativa L.) to Ducksalad (Heteranthera limosa)

More than 50 weed species infest drill-seeded rice in the U.S. and one of the most prevalent aquatic weeks is ducksalad (Heteranthera limosa). During the summer of 1988, a field experiment was conducted to identify rice accessions from the USDA/ARS rice germplasm collection for allelopathic effects to ducksalad. In this field experiment, 5,000 accessions were evaluated for allelopathic activity. Five to seven seeds of each rice accession were planted in hills about 75cm apart in two replications. Allelopathic activity was recorded as 1) radius of the area affected by allelochemical from the base of the rice plant and 2) percentage of weed control within the affected area. Ducksalad was rated at the panicle initiation stage of rice development. Of the 5,000 accessions that were evaluated, approximately 191 were identified as having evident allelopathic activity. The accessions that demonstrated allelopathic activity originated in 26 countries (Afghanistan, Argentina, Australia, Brazil, Columbia, Dominican Republic, France, India, Iran, Iraq, Israel, Italy, Japan, Malaysia, Mexico, Pakistan, Peru, Philippines, Portugal, Republic of Korea, People Republic of China, Soviet Union, Taiwan, Thailand, Turkey, and United States)

Sources and modes of action of invasive knotweed allelopathy : the effects of leaf litter and trained soil on the germination and growth of native plants

Invasive knotweeds, native to Eastern Asia, are among the most dominant plant invaders of European and North American temperate ecosystems. Recent studies indicate that one cause of this dominance might be allelopathy, but the possible sources and modes of action of this allelopathy are insufficiently understood. Here, we asked whether the invasive knotweed Fallopia × bohemica can exert allelopathic effects on native plants also through its leaf litter, or through persistent soil contaminants, and whether these affect the germination or growth of native plants. In a germination experiment with nine native species neither litter leachate, an aqueous extract of knotweed leaves added to the soil, nor trained soil with a history of Fallopia pre-cultivation suppressed the germination or early growth of natives. A mesocosm study with experimental native communities showed that the presence of F. × bohemica, although not a dominant in these communities, caused significant shifts of life-history strategy in two dominant natives, and that similar effects could be elicited through litter leachates or trained soil alone. However, there were hardly any effects on the biomass of natives. Our study indicates that knotweed allelopathy acts on the growth rather than germination of natives, and that soil contamination through persistent allelochemicals may not be a significant problem in habitat restoration. It also shows that allelopathic effects can sometimes be subtle changes in life-history and allocation patterns of the affected species

Potential phytotoxic and shading effects of invasive Fallopia (Polygonaceae) taxa on the germination of dominant native species

Two species of the genus Fallopia (F. sachalinensis, F. japonica, Polygonaceae) native to Asia, and their hybrid (F. ×bohemica), belong to the most noxious plant invaders in Europe. They impact highly on invaded plant communities, resulting in extremely poor native species richness. The low number of native species in invaded communities points to the possible existence of mechanisms suppressing their germination. In this study we assessed, under laboratory conditions, whether there are phytotoxic effects of the three Fallopia congeners on seed germination of three target species: two native species commonly growing in habitats that are often invaded by Fallopia taxa (Urtica dioica, Calamagrostis epigejos), and Lepidium sativum, a species commonly used in allelopathic bioassays as a control. Since Fallopia taxa form dense stands with high cover, we included varying light conditions as an additional factor, to simulate the effects of shading by leaf canopy on germination. The effects of aqueous extracts (2.5%, 5.0%, and 0% as a control) from dry leaves and rhizomes of the Fallopia congeners on germination of the target species were thus studied under two light regimes, simulating full daylight (white light) and light filtered through canopy (green light), and in dark as a control regime. Rhizome extracts did not affect germination. Light treatments yielded inconclusive results, indicating that poor germination and establishment of species in invaded stands is unlikely to be caused by shading alone. However, we found a pronounced phytotoxic effect of leaf extracts of Fallopia taxa, more so at 5.0% than 2.5% extract concentration. Fallopia sachalinensis exerted the largest negative effect on the germination of Urtica dioica, F. ×bohemica on that of C. epigejos, and F. japonica had invariably the lowest inhibitory effect on all test species. The weak phytotoxic effect of F. japonica corresponds to the results of previous studies that found this species to be generally a weaker competitor than its two congeners. Although these results do not necessarily provide direct evidence for allelopathic effects in the field, we demonstrate the potential phytotoxic effect of invasive Fallopia taxa on the germination of native species. This suggests that allelopathy may play a role in the impact of Fallopia invasion on species diversity of invaded communities

Potential phytotoxic and shading effects of invasive Fallopia (Polygonaceae) taxa on the germination of dominant native species

Two species of the genus Fallopia (F. sachalinensis, F. japonica, Polygonaceae) native to Asia, and their hybrid (F. ×bohemica), belong to the most noxious plant invaders in Europe. They impact highly on invaded plant communities, resulting in extremely poor native species richness. The low number of native species in invaded communities points to the possible existence of mechanisms suppressing their germination. In this study we assessed, under laboratory conditions, whether there are phytotoxic effects of the three Fallopia congeners on seed germination of three target species: two native species commonly growing in habitats that are often invaded by Fallopia taxa (Urtica dioica, Calamagrostis epigejos), and Lepidium sativum, a species commonly used in allelopathic bioassays as a control. Since Fallopia taxa form dense stands with high cover, we included varying light conditions as an additional factor, to simulate the effects of shading by leaf canopy on germination. The effects of aqueous extracts (2.5%, 5.0%, and 0% as a control) from dry leaves and rhizomes of the Fallopia congeners on germination of the target species were thus studied under two light regimes, simulating full daylight (white light) and light filtered through canopy (green light), and in dark as a control regime. Rhizome extracts did not affect germination. Light treatments yielded inconclusive results, indicating that poor germination and establishment of species in invaded stands is unlikely to be caused by shading alone. However, we found a pronounced phytotoxic effect of leaf extracts of Fallopia taxa, more so at 5.0% than 2.5% extract concentration. Fallopia sachalinensis exerted the largest negative effect on the germination of Urtica dioica, F. ×bohemica on that of C. epigejos, and F. japonica had invariably the lowest inhibitory effect on all test species. The weak phytotoxic effect of F. japonica corresponds to the results of previous studies that found this species to be generally a weaker competitor than its two congeners. Although these results do not necessarily provide direct evidence for allelopathic effects in the field, we demonstrate the potential phytotoxic effect of invasive Fallopia taxa on the germination of native species. This suggests that allelopathy may play a role in the impact of Fallopia invasion on species diversity of invaded communities

The search for exudates from Eurasian watermilfoil and hydrilla

Secondary metabolites are produced by aquatic plants, and in some instances, exudation of these metabolites into the surrounding water has been detected. To determine whether infestations of Eurasian watermilfoil or hydrilla produce such exudates, plant tissues and water samples were collected from laboratory cultures and pond populations and were analyzed using solid phase extraction, HPLC, and various methods of mass spectrometry including electrospray ionization, GC/MS, electron impact and chemical ionization. Previously reported compounds such as tellimagrandin II (from Eurasian watermilfoil) and a caffeic acid ester (from hvdrilla), along with a newly discovered flavonoid, cyanidin 3 dimalonyl glucoside (from hydrilla), were readily detected in plant tissues used in this research but were not detected in any of the water samples. If compounds are being released, as suggested by researchers using axenic cultures, we hypothesize that they may be rapidly degraded by bacteria and therefore undetectable

Proceedings of the COST SUSVAR/ECO-PB Workshop on organic plant breeding strategies and the use of molecular markers

In many countries,national projects are in progress to investigate the sustainable low-input approach.In the present COST network,these projects are coordinated by means of exchange of materials,establishing common methods for assessment and statistical analyses and by combining national experimental results.The common framework is cereal production in low-input sustainable systems with emphasis on crop diversity.The network is organised into six Working Groups,five focusing on specific research areas and one focusing on the practical application of the research results for variety testing:1)plant genetics and plant breeding,2)biostatistics,3)plant nutrition and soil microbiology,4)weed biology and plant competition,5)plant pathology and plant disease resistance biology and 6)variety testing and certification.It is essential that scientists from many disciplines work together to investigate the complex interactions between the crop and its environment,in order to be able to exploit the natural regulatory mechanisms of different agricultural systems for stabilising and increasing yield and quality.The results of this cooperation will contribute to commercial plant breeding as well as official variety testing,when participants from these areas disperse the knowledge achieved through the EU COST Action

Combination of Spatial and Temporal Diversification in European Cropping Systems

There is a lack of results on the advantages and limitations of combining different crop diversification strategies both in time and space, which makes it difficult for famers and advisers to find relevant information for the transition towards more diversified cropping systems. A network of ten field experiments (diverIMPACTS project) was built across seven European countries, covering a range of pedo-climatic conditions and different farming systems: arable and vegetable systems under both conventional and organic management. Each field experiment tests one or several diversified cropping systems, which combine three diversification strategies with low input practices. These diversified cropping systems are compared to reference systems, which are less diversified and more dependent on external inputs. The three strategies of crop diversification are rotation, multiple cropping (growing different crop species on the same land within one growing season) and intercropping (growing different species in proximity on the same field). A diversified system includes, for example, the addition of cover crops or cash crops, such as legumes, for their expected ecosystem services, or crops for new markets (e.g hemp, lentil), the use of multiple cropping to increase productivity per year (e.g. winter barley with soybean) and intercropping (e.g. barley/pea,wheat/faba bean, oat/lupin) to increase productivity per unit of area and reduce external inputs. Expected impacts include: higher arable land productivity, diversification and increased farmer revenues through access to new markets and reduced economic risk, lower environmental impact through reduced use of pesticides, chemical fertilisers, energy and water, and improved delivery of ecosystem services, including biodiversity. The diversified cropping systems will be assessed using standardized measurements across the network and multi-criteria assessment tools. Decisions regarding the design and management of the diversified cropping systems will be recorded to support other diversification initiatives. The year 2018 is the first year of the network. This paper presents the original approach, the strategies designed in the network, and the assumptions concerning the interests to combine temporal and spatial diversification in order to improve the delivery of multiple services. This network will be a source of inspiration for other initiatives of crop diversification in Europe. The ultimate goal is to guide farmers in their transition towards more diversified cropping systems and to promote innovations by various actors at different scales (e.g. innovations regarding machinery for sowing or harvesting new sole or mixed crops, value-chains through the consolidation of new markets, new process of transformation, or adaptation of value-chains to intercropping)

Potent endogenous allelopathic compounds in Lepidium sativum seed exudate: effects on epidermal cell growth in Amaranthus caudatus seedlings

Many plants exude allelochemicals – compounds that affect the growth of neighbouring plants. This study reports further studies of the reported effect of cress (Lepidium sativum) seed(ling) exudates on seedling growth in Amaranthus caudatus and Lactuca sativa. In the presence of live cress seedlings, both species grew longer hypocotyls and shorter roots than cress-free controls. The effects of cress seedlings were allelopathic and not due to competition for resources. Amaranthus seedlings grown in the presence of cress allelochemical(s) had longer, thinner hypocotyls and shorter, thicker roots – effects previously attributed to lepidimoide. The active principle was more abundant in cress seed exudate than in seedling (root) exudates. It was present in non-imbibed seeds and releasable from heat-killed seeds. Release from live seeds was biphasic, starting rapidly but then continuing gradually for 24 h. The active principle was generated by aseptic cress tissue and was not a microbial digestion product or seed-treatment chemical. Crude seed exudate affected hypocotyl and root growth at ∼25 and ∼450 μg ml(−1) respectively. The exudate slightly (28%) increased epidermal cell number along the length of the Amaranthus hypocotyl but increased total hypocotyl elongation by 129%; it resulted in a 26% smaller hypocotyl circumference but a 55% greater epidermal cell number counted round the circumference. Therefore, the effect of the allelochemical(s) on organ morphology was imposed primarily by regulation of cell expansion, not cell division. It is concluded that cress seeds exude endogenous substances, probably including lepidimoide, that principally regulate cell expansion in receiver plants

Assessment Method for Leaf Litters Allelopathic Effect on Cyanobacteria

A new bioassay technique combining leaf disk and softagar over-layer methods was developed to investigate the allelopathic effect of deciduous leaf litters on the growth of cyanobacteria ( Microcystis aeruginosa Kütz.). Bioactive substances exuded from leaf disks caused inhibitory plaques on the agar plate containing cyanobacteria , and the rate of diffusion depended on the specific leaf disk area. Most of the leaf litters collected around reservoirs in Japan showed inhibitory activity to M. aeruginosa , with Rhus trichocarpa Miq., Quercus variabilis Blume and Mallotus japonicus (Thunb.) Muell. Arg. being the strongest among the 22 tested species.(PDF has 4 pages.

Allelopathic effect of the Cladonia verticillaris lichen extracts and fumarprotocetraric acid on the early growth of germinated seedlings in Allium cepa L.

The allelopathic activity of the different type of Cladonia verticillaris lichen extracts and fumarprotocetraric acid on the early growth of A. cepa (IPA 6) germinated seedlings depends on their chemical composition and concentration, respectively. It was observed that the length of the radicle was significantly stimulated by fumarprotocetraric acid at high concentrations and by the total extract of C. verticillaris thalli, which contained high level of fumarprotocetraric, acid confirmed by HPLC – technique. In addition, it was found, that the phosphate buffer extract, which contained high level of methy betha-orcinol carboxilate measured by HPLC, significantly reduced the length of the hypocotyls. Under our experimental conditions there was no influence of different type of extract and fumarprotocetraric acid on the seed germination ratio of A. cepa, in relation to control. From the study of HPLC it was found that fumarprotocetraric acid and methy betha-orcinol carboxilate were present in all extracts at different concentrations, according to the method of extraction