Identification of Gastroenteric Viruses by Electron Microscopy Using Higher Order Spectral Features

Background: Many paediatric illnesses are caused by viral agents, for example, acute gastroenteritis. Electron microscopy can provide images of viral particles and can be used to identify the agents. Objectives: The use of electron microscopy as a diagnostic tool is limited by the need for high level of expertise in interpreting these images and the time required. A semi-automated method is proposed in this paper. Study design: The method is based on bispectal features that capture contour and texture information while providing robustness to shift, rotation, changes in size and noise. The magnification or true size of the viral particles need not be known precisely, but if available can be used additionally for improved classification. Viral particles from one or more images are segmented and analyzed to verify whether they belong to a particular class (such as Adenovirus, Rotavirus, etc.) or not. Two experiments were conducted—depending on the populations from which virus particle images were collected for training and testing, respectively. In the first, disjoint subsets from a pooled population of virus particles obtained from several images were used. In the second, separate populations from separate images were used. The performance of the method on viruses of similar size was separately evaluated using Astrovirus, HAV and Poliovirus. A Gaussian Mixture Model was used for the probability density of the features. A threshold on the log-likelihood is varied to study false alarm and false rejection trade-off. Features from many particles and/or likelihoods from independent tests are averaged to yield better performance. Results: An equal error rate (EER) of 2% is obtained for verification of Rotavirus (tested against three other viruses) when features from 15 viral particle images are averaged. It drops further to less than 0.2% when scores from two tests are averaged to make a decision. For verification of Astrovirus (tested against two others of the same size) the EER was less than 2% when 20 particles and two tests were used. Conclusion: Bispectral features and Gaussian mixture modelling of their probability density are shown to be effective in identifying viruses from electron microscope images. With the use of digital imaging in electron microscopes, this method can be fully automated

A new 2D static hand gesture colour image dataset for ASL gestures

It usually takes a fusion of image processing and machine learning algorithms in order to build a fully-functioning computer vision system for hand gesture recognition. Fortunately, the complexity of developing such a system could be alleviated by treating the system as a collection of multiple sub-systems working together, in such a way that they can be dealt with in isolation. Machine learning need to feed on thousands of exemplars (e.g. images, features) to automatically establish some recognisable patterns for all possible classes (e.g. hand gestures) that applies to the problem domain. A good number of exemplars helps, but it is also important to note that the efficacy of these exemplars depends on the variability of illumination conditions, hand postures, angles of rotation, scaling and on the number of volunteers from whom the hand gesture images were taken. These exemplars are usually subjected to image processing first, to reduce the presence of noise and extract the important features from the images. These features serve as inputs to the machine learning system. Different sub-systems are integrated together to form a complete computer vision system for gesture recognition. The main contribution of this work is on the production of the exemplars. We discuss how a dataset of standard American Sign Language (ASL) hand gestures containing 2425 images from 5 individuals, with variations in lighting conditions and hand postures is generated with the aid of image processing techniques. A minor contribution is given in the form of a specific feature extraction method called moment invariants, for which the computation method and the values are furnished with the dataset

New Method for Optimization of License Plate Recognition system with Use of Edge Detection and Connected Component

License Plate recognition plays an important role on the traffic monitoring and parking management systems. In this paper, a fast and real time method has been proposed which has an appropriate application to find tilt and poor quality plates. In the proposed method, at the beginning, the image is converted into binary mode using adaptive threshold. Then, by using some edge detection and morphology operations, plate number location has been specified. Finally, if the plat has tilt, its tilt is removed away. This method has been tested on another paper data set that has different images of the background, considering distance, and angel of view so that the correct extraction rate of plate reached at 98.66%.Comment: 3rd IEEE International Conference on Computer and Knowledge Engineering (ICCKE 2013), October 31 & November 1, 2013, Ferdowsi Universit Mashha