Complex regulation of the aflatoxin biosynthesis gene cluster of Aspergillus flavus in relation to various combinations of water activity and temperature

A microarray analysis was performed to study the effect of varying combinations of water activity and temperature on the activation of aflatoxin biosynthesis genes in Aspergillus flavus grown on YES medium. Generally A. flavus showed expression of the aflatoxin biosynthetic genes at all parameter combinations tested. Certain combinations of aw and temperature, especially combinations which imposed stress on the fungus resulted in a significant reduction of the growth rate. At these conditions induction of the whole aflatoxin biosynthesis gene cluster occurred, however the produced aflatoxin B1 was low. At all other combinations (25 °C/0.95 and 0.99; 30 °C/0.95 and 0.99; 35 °C/0.95 and 0.99) a reduced basal level of cluster gene expression occurred. At these combinations a high growth rate was obtained as well as high aflatoxin production. When single genes were compared, two groups with different expression profiles in relation to water activity/temperature combinations occurred. These two groups were co-ordinately localized within the aflatoxin gene cluster. The ratio of aflR/aflJ expression was correlated with increased aflatoxin biosynthesis

Moments of unconditional logarithmically concave vectors

We derive two-sided bounds for moments of linear combinations of coordinates od unconditional log-concave vectors. We also investigate how well moments of such combinations may be approximated by moments of Gaussian random variables.Comment: 14 page

Different adaptation strategies of two citrus scion/rootstock combinations in response to drought stress

Scion/rootstock interaction is important for plant development and for breeding programs. In this context, polyploid rootstocks presented several advantages, mainly in relation to biotic and abiotic stresses. Here we analyzed the response to drought of two different scion/rootstock combinations presenting different polyploidy: the diploid (2x) and autotetraploid (4x) Rangpur lime (Citrus limonia, Osbeck) rootstocks grafted with 2x Valencia Delta sweet orange (Citrus sinensis) scions, named V/2xRL and V/4xRL, respectively. Based on previous gene expression data, we developed an interactomic approach to identify proteins involved in V/2xRL and V/4xRL response to drought. A main interactomic network containing 3,830 nodes and 97,652 edges was built from V/2xRL and V/4xRL data. Exclusive proteins of the V/2xRL and V/4xRL networks (2,056 and 1,001, respectively), as well as common to both networks (773) were identified. Functional clusters were obtained and two models of drought stress response for the V/2xRL and V/4xRL genotypes were designed. Even if the V/2xRL plant implement some tolerance mechanisms, the global plant response to drought was rapid and quickly exhaustive resulting in a general tendency to dehydration avoidance, which presented some advantage in short and strong drought stress conditions, but which, in long terms, does not allow the plant survival. At the contrary, the V/4xRL plants presented a response which strong impacts on development but that present some advantages in case of prolonged drought. Finally, some specific proteins, which presented high centrality on interactomic analysis were identified as good candidates for subsequent functional analysis of citrus genes related to drought response, as well as be good markers of one or another physiological mechanism implemented by the plants. (Résumé d'auteur

A quasi-linear control theory analysis of timesharing skills

Performance with practice on two dual-task combinations, dual-axis tracking and two discrete information processing tasks, is examined in an effort to identify the presence and development of specific time sharing skills, such as parallel information processing or rapid intertask switching. The generality of time sharing skills also is investigated by examining transfer of these skills between the two qualitatively different task combinations

Combinations of LL-functions and Their Non-coincident Zeros for σ>1\sigma>1

The purpose of this note is to build upon work of Booker--Thorne and Righetti concerning zeros of algebraic combinations of $L$-functions. Namely, we show that two generic combinations of functions from a wide class of Euler products have non-coincident zeros in the half-plane $\sigma>1$.Comment: 6 page

Disentanglement and decoherence in two-spin and three-spin systems under dephasing

We compare disentanglement and decoherence rates within two-spin and three-spin entangled systems subjected to all possible combinations of local and collective pure dephasing noise combinations. In all cases, the bipartite entanglement decay rate is found to be greater than or equal to the dephasing-decoherence rates and often significantly greater. This sharpens previous results for two-spin systems [T. Yu and J. H. Eberly Phys. Rev. B 68, 165322 (2003)] and extends them to the three-spin context.Comment: 17 page

Interactions of Tollmien-Schlichting waves and Dean vortices. Comparison of direct numerical simulation and a weakly nonlinear theory

Direct numerical simulation is used to evaluate a weakly nonlinear theory describing the interaction of Tollmien-Schlichting waves with Dean vortices in curved channel flow. The theory and the simulation agree for certain combinations of parameters, but the two approaches give conflicting results for other combinations. Some possibilities for these discrepancies are discussed