25,453 research outputs found
Tensegrity and Motor-Driven Effective Interactions in a Model Cytoskeleton
Actomyosin networks are major structural components of the cell. They provide
mechanical integrity and allow dynamic remodeling of eukaryotic cells,
self-organizing into the diverse patterns essential for development. We provide
a theoretical framework to investigate the intricate interplay between local
force generation, network connectivity and collective action of molecular
motors. This framework is capable of accommodating both regular and
heterogeneous pattern formation, arrested coarsening and macroscopic
contraction in a unified manner. We model the actomyosin system as a motorized
cat's cradle consisting of a crosslinked network of nonlinear elastic filaments
subjected to spatially anti-correlated motor kicks acting on motorized (fibril)
crosslinks. The phase diagram suggests there can be arrested phase separation
which provides a natural explanation for the aggregation and coalescence of
actomyosin condensates. Simulation studies confirm the theoretical picture that
a nonequilibrium many-body system driven by correlated motor kicks can behave
as if it were at an effective equilibrium, but with modified interactions that
account for the correlation of the motor driven motions of the actively bonded
nodes. Regular aster patterns are observed both in Brownian dynamics
simulations at effective equilibrium and in the complete stochastic
simulations. The results show that large-scale contraction requires correlated
kicking.Comment: 38 pages, 13 figure
Contact inhibition of locomotion and mechanical cross-talk between cell-cell and cell-substrate adhesion determines the pattern of junctional tension in epithelial cell aggregates
We generated a computational approach to analyze the biomechanics of
epithelial cell aggregates, either island or stripes or entire monolayers, that
combines both vertex and contact-inhibition-of-locomotion models to include
both cell-cell and cell-substrate adhesion. Examination of the distribution of
cell protrusions (adhesion to the substrate) in the model predicted high order
profiles of cell organization that agree with those previously seen
experimentally. Cells acquired an asymmetric distribution of basal protrusions,
traction forces and apical aspect ratios that decreased when moving from the
edge to the island center. Our in silico analysis also showed that tension on
cell-cell junctions and apical stress is not homogeneous across the island.
Instead, these parameters are higher at the island center and scales up with
island size, which we confirmed experimentally using laser ablation assays and
immunofluorescence. Without formally being a 3-dimensional model, our approach
has the minimal elements necessary to reproduce the distribution of cellular
forces and mechanical crosstalk as well as distribution of principal stress in
cells within epithelial cell aggregates. By making experimental testable
predictions, our approach would benefit the mechanical analysis of epithelial
tissues, especially when local changes in cell-cell and/or cell-substrate
adhesion drive collective cell behavior.Comment: 39 pages, 8 Figures. Supplementary Information is include
- …