Penambahan Tenebrio molitor Pada Pakan Terhadap Karakteristik Hedonik dan Mutu Hedonik Daging Ayam Mentah dan Matang

ABSTRACT Tenebrio molitor is an insect larvae that has the potential as a protein source for poultry feed. However, no exploratory research has been found on the quality of poultry meat reared with feed containing these insect larvae. The purpose of this study was to evaluate the hedonic and hedonic quality characteristics of fresh and boiled chicken meat in terms of color, texture, aroma and taste of broiler chicken fed with Tenebrio molitor meal. This research method is experimental using a completely randomized design (CRD). As a treatment, there were two types of feed, namely feed containing 5% MBM as control (R0) and feed containing 5% Tenebrio molitor meal (R1). The treatment was repeated five times, ten chickens for each replication. The results of hedonic and hedonic quality observations were analyzed descriptively. Based on hedonic quality characteristics; the color of the raw meat is redder, the aroma is less fishy, and the taste of cooked meat is more savory. And, in hedonic; broiler chicken that is fed a feed containing Tenebrio molitor meal is preferred by the panelists. So it can be concluded that broiler chicken meat that is fed with Tenebrio molitor meal is better than chicken meat that consumes MBM. Key Words: Chicken meat, Hedonic,Tenebrio molito

The Visual Mechanisms of Tenebrio Molitor: Changes in the Electro-Retinogram as Function of the Stimulus Duration

The ERG complex of the compound eye of the yellow mealworm bettle Tenebrimolitor (L.) separates into two components with stimulus durations of longer than 100 ms. These are the ‘on’ and the ‘off’ effects. Above stimulus durations of 1 s the positive potential following the ‘on’ is faster and the positive ‘off’ is followed by a small negative one. The latent period of the ‘on’ is independent of the stimulus duration while for stimulus durations of longer than 300 ms the ‘off’ latency is coupled with the end of the stimulus. If measured after the extinction of the stimulus the ‘off’ latency is affected by the stimulus duration. The ‘on’ and ‘off’ amplitudes behave similarly for various stimulus durations. Both originate in the receptor cells. The Bunsen-Roscoe Law of photochemistry holds for stimulus durations of 10-40 ms. For a given intensity, the amplitudes diminished for durations longer than 300 ms

Pemanfaatan Limbah Organik Sebagai Media Pakan Tenebrio molitor

Penelitian bertujuan untuk mengetahui pakan limbah organik yang paling optimal sebagai media pakan Tenebrio molitor. Pendekatan pada penelitian berupa pendekatan kuantitatif. Jenis penelitian eksperimen dengan Rancangan Acak Lengkap (RAL) terdiri dari  3 perlakuan dan 6  pengulangan digunakan untuk penelitian larva Tenebrio molitor P1 (limbah sayur), P2 (limbah ikan), P3(limbah sayur+limbah ikan). Parameter dalam penelitian ini adalah mortalitas larva, konsumsi larva Tenebrio molitor, pertambahan bobot tubuh larva. Data dianalisis menggunakan uji Anova Dua Jalur (two way) dan uji lanjut Jarak Nyata Duncan pada taraf signifikan 0,05. Hasil penelitian menunjukkan bahwa konsumsi larva pada pakan limbah berpengaruh dan saling berinteraksi dengan umur larva. Hal ini menunjukkan Fhitung (6,52) >Ftabel (2,10). Dari hasil penelitian dapat disimpulkan bahwa pakan yang paling optimal sebagai media pakan Tenebrio molitor adalah P3 (limbah sayur+limbah ikan) karena memiliki rerata konsumsi makan tertinggi dengan tingkat mortalitas hanya mencapai 47%. Pertambahan bobot tubuh yang konsisten selalu meningkat dan tertinggi di bandingkan perlakuan yang lain

Influence of Tenebrio molitor L Supplementation on Egg Quality and Omega-3 Content

Tenebrio molitor L is one of the alternative feed ingredients because it is rich in nutrients, namely protein, vitamins, minerals (calcium), energy, and fat. Tenebrio molitor L also contains 33.64±0.22% omega-3, so it is hoped that the eggs produced contain omega-3. In this study 300 Lohman Brown laying hens of 20-week-old were used. Completely randomized design (CRD) was applied in this study with 3 treatments and 10 replications, each replication contained 10 laying hens. Treatments were: P0= Feed containing 5% MBM, P1= Feed containing 2.5% MBM + 2.5% Tenebrio molitor L, and P2= Feed containing 5% Tenebrio molitor L. This research was conducted for 6 months. The variables observed were egg production, egg weight, egg shape index, shell weight, shell thickness, Haugh unit, yolk index, and omega-3. Treatment had no influence on egg physical quality but had a significant influence on egg weight. Treatment P0 prodeced the lowest egg weight that was 59.02±0.53 g. Treatment P2 had higher omega-3 contents than P0 and P1 that was 88±0.12 mg 100 g-1. It was concluded that Tenebrio molitor L could replace MBM up to 5% in laying hens feed, improve eggs quality, and omega-3 content in eggs

Effect of dietary supplementation with insect fats on growth performance, digestive efficiency and health of rabbits

Background: The present work aimed at evaluating the effect of the dietary replacement of soybean oil (S) by two types of insect fats extracted from black soldier fly larvae (H, Hermetia illucens L.) and yellow mealworm larvae (T, Tenebrio molitor L.) on growth performance, nutrient digestibility, blood parameters, intestinal morphology and health of growing rabbits. Methods: At weaning, 200 crossbred rabbits (36 days old) were allotted to five dietary treatments (40 rabbits/group): a control diet (C) containing 1.5% of soybean oil and four experimental diets where soybean oil was partially (50%) or totally (100%) substituted by H (H50 and H100) or T (T50 and T100) fats. Total tract digestibility was evaluated on 12 rabbits per treatment. The growth trial lasted 41 d and, at slaughtering (78 days old), blood samples were collected from 15 rabbits per treatment, morphometric analyses were performed on duodenum, jejunum and ileum mucosa, and samples of liver, spleen and kidney were submitted to histological evaluation. Results: No difference was observed between the control and the experimental groups fed insect fats in terms of performance, morbidity, mortality and blood variables. The addition of H and T fats did not influence apparent digestibility coefficients of dry matter, protein, ether extract, fibre fractions and gross energy. Gut morphometric indices and organ histopathology were not affected by dietary inclusion of H and T fats. Conclusions: H and T fats are suitable sources of lipid in rabbit diets to replace soybean oil without any detrimental effect on growth performance, apparent digestibility, gut mucosa traits and health

An assessment of the antibacterial activity in larval excretion/secretion of four species of insects recorded in association with corpses, using Lucilia sericata Meigen as the marker species

The relative antibacterial activities of excretion/secretion (ES) from two carrion-feeding insects, Calliphora vicina Robineau-Desvoidy and Dermestes maculatus DeGeer, and a detritivore, Tenebrio molitor Linnaeus, were compared to that of Lucilia sericata Meigen, a species with ES of known antibacterial capacity, in order to explore the antimicrobial potential of other carrion and detritivore species. Viable counts were used to assess time-kill of ES against five bacterial species, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Proteus mirabilis. Antibacterial activity was recorded in all four insect species although T. molitor and D. maculatus were the most effective in controlling growth of P. mirabilis. The blowflies were more effective in controlling a wider range of both Gram-positive and Gram-negative bacteria. The larval ES from all species was shown to reduce bacterial growth rate although differences in antibacterial spectrum were noted and the degree of potency varied between the four species. These differences may be explained ecologically by the different colonisation times of each insect species on the corpse. Overall, this study demonstrates that research into other carrion-feeding insect species has potential to provide an increased source of antimicrobial chemicals to broaden the range of bacterial species beyond that currently controlled using L. sericata

Determination of LC50 value of Nicotiana tabacum L. extract against Tenebrio molitor and Zophobas morio larvae

Tobacco has lots of active compounds that can be used as raw material for making natural insecticides. Tenebrio molitor and Zophobas morio are examples of agricultural pests. The purpose of this study was to determine LC50 values of tobacco extract on Tenebrio molitor and Zophobas morio. Previous research showed that tobacco extract had neurotoxin activity and nicotine was the highest content contained in the tobacco leaves. Tobacco extract was obtained by the Extended Heat Reflux Extraction method with ethanol solvent. LC50 values of tobacco extract for Tenebrio molitor and Zophobas morio were 21.1 mg/ml and 71.1 mg/ml, respectively

The symbiotic relationship between Tenebrio molitor and coffee grounds presents an innovative perspective on addressing global challenges

This study unveils a novel approach toward sustainable protein production and waste management by investigating the utilization of coffee grounds as a nutrient source for Tenebrio molitor larvae. This research is a pioneering exploration in response to pressing protein shortages, food waste, and food security challenges. The study employs an entirely randomized experimental design, comprising five treatment groups and one control group; each replicated thrice. Notably, the study amalgamates cutting-edge data mining techniques through the Scopus database to elucidate the existing research landscape around Tenebrio molitor. This exploration underscores the insect\u27s pivotal role as a prospective alternative protein source. The experimental findings, a core contribution, discern that T1, a blend of 5% coffee grounds and 95% substrate, yields the most significant mass gain in larvae over the initial 7-day phase, surpassing the control group. However, an intriguing revelation follows: specific treatments experience subsequent weight loss, emphasizing the significance of strategic larval harvesting and processing. These collective results underscore Tenebrio Molitor\u27s potential as an innovative protein source while spotlighting coffee grounds as a valuable larval growth catalyst. This study forges a path towards sustainable protein solutions, elucidating the dynamic interplay between Tenebrio molitor, coffee grounds, and their promising role in addressing food security concerns, particularly in socioeconomically challenged regions

Особенности белково-пептидного и углеводного состава супернатантов из личинок Tenebrio molitor после холодовой акклимации

В работе хроматографическим методом исследовали молекулярно-массовое распределение белков и пептидов из супернатантов, полученных из личинок Tenebrio molitor после холодовой акклимации. Наибольшее количество пептидных фракций имеют супернатанты из неакклимированных к холоду личинок T. molitor. Показано, что холодоакклимированные личинки T. molitor содержат низкомолекулярные пептидные фракции с м. м. (540 ± 20) – (2255 ± 85) Да, а неакклимированные – значительное количество высокомолекулярных пептидов с м. м. (4675 ± 225) – (6595 ± 550) Да. Определено содержание сахаров и сахароспиртов в супернатантах акклимированных к холоду и неакклимированных личинок T. molitor. Установлено, что концентрация глюкозы у неакклимированных особей T. molitor в 1,34 раза выше, чем у холодоакклимированных. Акклимированные личинки T. molitor содержали большее количество гидрофильных и меньшее количество гидрофобных белков по сравнению с неакклимированными. Также обнаружено, что неакклимированные личинки T. molitor содержали низкие концентрации сахароспиртов (сорбитола и маннитола), которые отсутствовали у акклимированных.У роботі хроматографічним методом досліджували молекулярно-масовий розподіл білків і пептидів із супернатантів, отриманих із личинок Tenebrio molitor після холодової аклімації. Найбільшу кількість пептидних фракцій мають супернатанти із неаклімованих до холоду личинок T. molitor. Показано, що холодоаклімовані личинки T. molitor містять низькомолекулярні пептидні фракції з м. м. (540 ± 20) – (2255 ± 85) Да, а неаклімовані – значну кількість високомолекулярних пептидів з м. м. (4675 ± 225) – (6595 ± 550) Да. Визначено вміст цукру та цукроспиртів у супернатантах аклімованих до холоду та неаклімованих личинок T. molitor. Встановлено, що концентрація глюкози у неаклімованих личинок T. molitor була в 1,34 рази вище, ніж у холодоаклімованих. Аклімовані личинки T. molitor мали більшу кількість гідрофільних і меншу кількість гідрофобних білків у порівнянні з неаклімованими. Також виявлено, що неаклімовані личинки T. molitor мали низькі концентрації цукроспиртів (сорбітолу та манітолу), які були відсутні у аклімованих.The paper describes the chromatographic studies on the molecular-mass distribution of proteins and peptides of supernatants, derived from Tenebrio molitor larvae during cold acclimation. Supernatants of non-acclimated larvae of T. molitor had the highest amount of peptide fractions. It has been shown that cold-acclimated T. molitor larvae comprised the low-molecular peptide fractions with MW of (540 ± 20) – (2.255 ± 85) Da, and high-molecular peptides with MW of (4.675 ± 225) – (6.595 ± 550) Da were characteristic for non-acclimated larvae. The content of sugars and polyols in supernatants of cold-acclimated and non-acclimated T. molitor larvae was determined. It has been found that glucose concentration in non-acclimated T. molitor larvae was in 1.34 times higher than in cold-acclimated ones. Аcclimated T. molitor larvae had more hydrophilic and less hydrophobic proteins as compared to non-acclimated ones. As well it has been established that non-acclimated T. molitor larvae had low concentrations of polyols (sorbitol and mannite), which were absent in acclimated insects