3 research outputs found
The EcoChip : a wireless multi-sensor platform for comprehensive environmental monitoring
This paper presents the EcoChip, a new system
based on state-of-the-art electro-chemical impedance (EIS)
technologies allowing the growth of single strain organisms
isolated from northern habitats. This portable system is a complete
and autonomous wireless platform designed to monitor and
cultivate microorganisms directly sampled from their natural
environment, particularly from harsh northern environments.
Using 96-well plates, the EcoChip can be used in the field for realtime monitoring of bacterial growth. Manufactured with highquality electronic components, this new EIS monitoring system is
designed to function at a low excitation voltage signal to avoid
damaging the cultured cells. The high-precision calibration
network leads to high-precision results, even in the most limiting
contexts. Luminosity, humidity and temperature can also be
monitored with the addition of appropriate sensors. Access to
robust data storage systems and power supplies is an obvious
limitation for northern research. That is why the EcoChip is
equipped with a flash memory that can store data over long
periods of time. To resolve the power issue, a low-power microcontroller and a power management unit control and supply all
electronic building blocks. Data stored in the EcoChip’s flash
memory can be transmitted through a transceiver whenever a
receiver is located within the functional transmission range. In this
paper, we present the measured performance of the system, along
with results from laboratory tests in-vitro and from two field tests.
The EcoChip has been utilized to collect bio-environemental data
in the field from the northern soils and ecosystems of
Kuujjuarapik and Puvirnituq, during two expeditions, in 2017 and
2018, respectively. We show that the EcoChip can effectively carry
out EIS analyses over an excitation frequency ranging from 750
Hz to 10 kHz with an accuracy of 2.35%. The overall power
consumption of the system was 140.4 mW in normal operating
mode and 81 µW in sleep mode. The proper development of the
isolated bacteria was confirmed through DNA sequencing,
indicating that bacteria thrive in the EcoChip’s culture wells while
the growing conditions are successfully gathered and stored
L'EcoChip : une plateforme de capteurs autonomes pour la surveillance bio-environnementale multimodale de l'habitat nordique
Un grand intérêt est remarqué au sein de la communauté et les chercheurs scientifiques pour étudier le changement et le réchauffement climatique ainsi que pour évaluer les conditions environnementales actuelles et ses limites. Il est établi que la présence de micro-organismes sélectionnés dans un environnement spécifique peut prédire différents facteurs environnementaux dans un habitat donné. L'écologie microbienne étudie les relations existantes entre les micro-organismes et leur environnement. Comprendre les micro-organismes qui prospèrent dans ces régions nordiques peut grandement faire progresser nos connaissances sur ces habitats et les effets des changements climatiques. Ces microbes sont appelés bioindicateurs. Mais, à l'heure actuelle, les micro-organismes bioindicateurs qui se développent dans différents habitats qui sont les plus touchés par les changements climatiques, comme ceux des régions septentrionales du Canada, sont encore pour la plupart inconnus. Le programme transdisciplinaire Stratégie Sentinelle Nord de l'Université Laval vise à développer des technologies innovantes et à accroître la connaissance collective des habitats nordiques et de leurs impacts sur les êtres humains et leur santé. Sous l'égide de l'initiative Sentinel Nord, le projet EcoChip est un programme ambitieux qui vise à développer une technologie autonome de surveillance, de compréhension et de valorisation des microorganismes présents dans le Nord. La technologie EcoChip vise également à identifier des molécules uniques susceptibles d'être utilisées dans des processus biologiques et industriels. Ici, nous présentons la technologie EcoChip de nouvelle génération. C’est un système autonome qui permet de surveiller la croissance des micro-organismes dans les puits individuels sur le terrain. Il peut mesurer le taux de croissance des micro-organismes et leurs conditions environnementales in-situ grâce à une plate-forme de capteurs embarqués. Cette nouvelle version améliore de nombreux aspects du prototype précédent. Entre autres, il a un réseau d'électrodes EIS plaqué et intégré à la carte électronique, un nouveau boîtier hermétique personnalisé et a été fabriqué en utilisant une technologie de carte de circuit imprimé standard et peu coûteuse.The community and scientific researchers have great interest in studying climate change and warming as well as to assess current environmental conditions and its limitations. Microbial ecology studies the existing relationships between microorganisms and their environment. The presence of selected microorganisms in a specific environment can predict different environmental factors in each habitat. These sentinel microbes are called bioindicators. Understanding the microorganisms that thrive in these northern regions can greatly advance our knowledge of these habitats and the effects of climate change. However, at present, the bioindicator microorganisms that thrive in different habitats that are most affected by climate change, such as those in northern regions of Canada, are still mostly unknown. The transdisciplinary Sentinel North Strategy program aims to develop innovative technologies and increase collective knowledge of northern habitats and their impacts on humans and their health. Under the aegis of the Sentinel North initiative, this project is an ambitious program, aims to develop an autonomous technology for monitoring, understanding and valuing the microorganisms present in the North. EcoChip technology also aims to identify unique molecules used in biological and industrial processes. Here we present the next generation EcoChip technology. It is a stand-alone system built to monitor the growth of microorganisms in individual wells in the field. It can measure the growth rate of microorganisms and their environmental conditions in-situ using an on-board sensor platform. The system can measure the impedance of microorganisms inside 96 wells using the Electrochemical Impedance Spectroscopy (EIS) technique. Other parameters like temperature, humidity and luminosity are measured. The EcoChip has many improvements. Among other things, it has an EIS electrode array plated and integrated with the electronic board, a new customized hermetic package and was manufactured using standard and inexpensive printed circuit board technology