576 research outputs found
Modern meat: the next generation of meat from cells
Modern Meat is the first textbook on cultivated meat, with contributions from over 100 experts within the cultivated meat community.
The Sections of Modern Meat comprise 5 broad categories of cultivated meat: Context, Impact, Science, Society, and World.
The 19 chapters of Modern Meat, spread across these 5 sections, provide detailed entries on cultivated meat. They extensively tour a range of topics including the impact of cultivated meat on humans and animals, the bioprocess of cultivated meat production, how cultivated meat may become a food option in Space and on Mars, and how cultivated meat may impact the economy, culture, and tradition of Asia
Development of Bridge Information Model (BrIM) for digital twinning and management using TLS technology
In the current modern era of information and technology, the concept of Building Information Model (BIM), has made revolutionary changes in different aspects of engineering design, construction, and management of infrastructure assets, especially bridges. In the field of bridge engineering, Bridge Information Model (BrIM), as a specific form of BIM, includes digital twining of the physical asset associated with geometrical inspections and non-geometrical data, which has eliminated the use of traditional paper-based documentation and hand-written reports, enabling professionals and managers to operate more efficiently and effectively. However, concerns remain about the quality of the acquired inspection data and utilizing BrIM information for remedial decisions in a reliable Bridge Management System (BMS) which are still reliant on the knowledge and experience of the involved inspectors, or asset manager, and are susceptible to a certain degree of subjectivity. Therefore, this research study aims not only to introduce the valuable benefits of Terrestrial Laser Scanning (TLS) as a precise, rapid, and qualitative inspection method, but also to serve a novel sliced-based approach for bridge geometric Computer-Aided Design (CAD) model extraction using TLS-based point cloud, and to contribute to BrIM development. Moreover, this study presents a comprehensive methodology for incorporating generated BrIM in a redeveloped element-based condition assessment model while integrating a Decision Support System (DSS) to propose an innovative BMS. This methodology was further implemented in a designed software plugin and validated by a real case study on the Werrington Bridge, a cable-stayed bridge in New South Wales, Australia. The finding of this research confirms the reliability of the TLS-derived 3D model in terms of quality of acquired data and accuracy of the proposed novel slice-based method, as well as BrIM implementation, and integration of the proposed BMS into the developed BrIM. Furthermore, the results of this study showed that the proposed integrated model addresses the subjective nature of decision-making by conducting a risk assessment and utilising structured decision-making tools for priority ranking of remedial actions. The findings demonstrated acceptable agreement in utilizing the proposed BMS for priority ranking of structural elements that require more attention, as well as efficient optimisation of remedial actions to preserve bridge health and safety
The study of renal function and toxicity using zebrafish (Danio rerio) larvae as a vertebrate model
Zebrafish (Danio rerio) is a powerful model in biomedical and pharmaceutical sciences. The zebrafish model was introduced to toxicological sciences in 1960, followed by its use in biomedical sciences to investigate vertebrate gene functions. As a consequence of many research projects in this field, the study of human genetic diseases became instantly feasible. Consequently, zebrafish have been intensively used in developmental biology and associated disciplines. Due to the simple administration of medicines and the high number of offspring, zebrafish larvae became widely more popular in pharmacological studies in the following years. In the past decade, zebrafish larvae were further established as a vertebrate model in the field of pharmacokinetics and nanomedicines. In this PhD thesis, zebrafish larvae were investigated as an earlystage in vivo vertebrate model to study renal function, toxicity, and were applied in drug-targeting projects using nanomedicines.
The first part focused on the characterization of the renal function of three-to four-dayold zebrafish larvae. Non-renal elimination processes were additionally described. Moreover, injection techniques, imaging parameters, and post-image processing scripts were established to serve as a toolbox for follow-up projects.
The second part analyzed the impact of gentamicin (a nephrotoxin) on the morphology of the pronephros of zebrafish larvae. Imaging methodologies such as fluorescent-based laser scanning microscopy and X-ray-based microtomography were applied. A profound comparison study of specimens acquired with different laboratory X-ray-based microtomography devices and a radiation facility was done to promote the use of X-ray-based microtomography for broader biomedical applications.
In the third part, the toxicity of nephrotoxins on mitochondria in renal epithelial cells of proximal tubules was assessed using the zebrafish larva model. Findings were compared with other teleost models such as isolated renal tubules of killifish (Fundulus heteroclitus). In view of the usefulness and high predictability of the zebrafish model, it was applied to study the pharmacokinetics of novel nanoparticles in the fourth part. Various in vivo pharmacokinetic parameters such as drug release, transfection of mRNA/pDNA plasmids, macrophage clearance, and the characterization of novel drug carriers that were manipulated with ultrasound were assessed in multiple collaborative projects.
Altogether, the presented zebrafish model showed to be a reliable in vivo vertebrate model to assess renal function, toxicity, and pharmacokinetics of nanoparticles. The application of the presented model will hopefully encourage others to reduce animal experiments in preliminary studies by fostering the use of zebrafish larvae
LIPIcs, Volume 261, ICALP 2023, Complete Volume
LIPIcs, Volume 261, ICALP 2023, Complete Volum
Finding a Husband: Using Explainable AI to Define Male Mosquito Flight Differences
Mosquito-borne diseases account for around one million deaths annually. There is a constant need for novel intervention mechanisms to mitigate transmission, especially as current insecticidal methods become less effective with the rise of insecticide resistance among mosquito populations. Previously, we used a near infra-red tracking system to describe the behaviour of mosquitoes at a human-occupied bed net, work that eventually led to an entirely novel bed net design. Advancing that approach, here we report on the use of trajectory analysis of a mosquito flight, using machine learning methods. This largely unexplored application has significant potential for providing useful insights into the behaviour of mosquitoes and other insects. In this work, a novel methodology applies anomaly detection to distinguish male mosquito tracks from females and couples. The proposed pipeline uses new feature engineering techniques and splits each track into segments such that detailed flight behaviour differences influence the classifier rather than the experimental constraints such as the field of view of the tracking system. Each segment is individually classified and the outcomes are combined to classify whole tracks. By interpreting the model using SHAP values, the features of flight that contribute to the differences between sexes are found and are explained by expert opinion. This methodology was tested using 3D tracks generated from mosquito mating swarms in the field and obtained a balanced accuracy of 64.5% and an ROC AUC score of 68.4%. Such a system can be used in a wide variety of trajectory domains to detect and analyse the behaviours of different classes, e.g., sex, strain, and species. The results of this study can support genetic mosquito control interventions for which mating represents a key event for their success
Tree Structure Retrieval for Apple Trees from 3D Pointcloud
3D reconstruction is a challenging problem and has been an important research topic in the areas of remote sensing and computer vision for many years. Existing 3D reconstruction approaches are not suitable for orchard applications due to complicated tree structures. Current tree reconstruction has included models specific to trees of a certain density, but the impact of varying Leaf Area Index(LAI) on model performance has not been studied.
To better manage an apple orchard, this thesis proposes methods for evaluating an apple canopy density mapping system as an input for a variable-rate sprayer for both trellis-structured (2D) and standalone (3D) apple orchards using a 2D LiDAR (Light Detection and Ranging). The canopy density mapping system has been validated for robustness and repeatability with multiple scans. The consistency of the whole row during multiple passes has a correlation R^2 = 0.97. The proposed system will help the decision-making in a variable-rate sprayer.
To further study the individual tree structure, this thesis proposes a novel and fast approach to reconstruct and analyse 3D trees over a range of Leaf Area Index (LAI) values from LiDAR for morphology analysis for height, branch length and angles of real and simulated apple trees. After using Principal Component Analysis (PCA) to extract the trunk points, an improved Mean Shift algorithm is introduced as Adapted Mean Shift (AMS) to classify different branch clusters and extract the branch nodes.
A full evaluation workflow of tree parameters including trunk and branches is introduced for morphology analysis to investigate the accuracy of the approach over different LAI values. Tree height, branch length, and branch angles were analysed and compared to the ground truth for trees with a range of LAI values. When the LAI is smaller than 0.1, the accuracy for height and length is greater than 90\% and the accuracy for the angles is around 80\%. When the LAI is greater than 0.1, the branch accuracy reduces to 40\%. This analysis of tree reconstruction performance concerning LAI values, as well as the combination of efficient and accurate structure reconstruction, opens the possibility of improving orchard management and botanical studies on a large scale.
To improve the accuracy of traditional tree structure analysis, a deep learning approach is introduced to pre-process and classify unbalanced, in-homogeneous, and noisy point cloud data. TreeNet is inspired by 3D U-Net, adding classes and median filters to segment trunk, branch, and leave parts. TreeNet outperformed 3D U-Net and SVM in the case of Kappa, Matthews Correlation Coefficient(MCC), and F1-score value in segmentation. The TreeNet-AMS combined method also showed improvement in tree structure analysis than the traditional AMS method mentioned above.
Following on from this research, efficient tree structure analysis on tree height, trunk length, branch position, and branch length could be conducted. Knowing the tree morphology is proved to be closely relevant to thinning, spraying and yield, the proposed work will then largely benefit the relevant studies in agriculture and forestry
Cyber-Human Systems, Space Technologies, and Threats
CYBER-HUMAN SYSTEMS, SPACE TECHNOLOGIES, AND THREATS is our eighth textbook in a series covering the world of UASs / CUAS/ UUVs / SPACE. Other textbooks in our series are Space Systems Emerging Technologies and Operations; Drone Delivery of CBNRECy – DEW Weapons: Emerging Threats of Mini-Weapons of Mass Destruction and Disruption (WMDD); Disruptive Technologies with applications in Airline, Marine, Defense Industries; Unmanned Vehicle Systems & Operations On Air, Sea, Land; Counter Unmanned Aircraft Systems Technologies and Operations; Unmanned Aircraft Systems in the Cyber Domain: Protecting USA’s Advanced Air Assets, 2nd edition; and Unmanned Aircraft Systems (UAS) in the Cyber Domain Protecting USA’s Advanced Air Assets, 1st edition. Our previous seven titles have received considerable global recognition in the field. (Nichols & Carter, 2022) (Nichols, et al., 2021) (Nichols R. K., et al., 2020) (Nichols R. , et al., 2020) (Nichols R. , et al., 2019) (Nichols R. K., 2018) (Nichols R. K., et al., 2022)https://newprairiepress.org/ebooks/1052/thumbnail.jp
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