6,873 research outputs found
A Method for Unsupervised Semi-Quantification of Inmunohistochemical Staining with Beta Divergences
In many research laboratories, it is essential to determine the relative expression levels of
some proteins of interest in tissue samples. The semi-quantitative scoring of a set of images consists
of establishing a scale of scores ranging from zero or one to a maximum number set by the researcher
and assigning a score to each image that should represent some predefined characteristic of the IHC
staining, such as its intensity. However, manual scoring depends on the judgment of an observer and
therefore exposes the assessment to a certain level of bias. In this work, we present a fully automatic
and unsupervised method for comparative biomarker quantification in histopathological brightfield
images. The method relies on a color separation method that discriminates between two chromogens
expressed as brown and blue colors robustly, independent of color variation or biomarker expression
level. For this purpose, we have adopted a two-stage stain separation approach in the optical density
space. First, a preliminary separation is performed using a deconvolution method in which the color
vectors of the stains are determined after an eigendecomposition of the data. Then, we adjust the
separation using the non-negative matrix factorization method with beta divergences, initializing
the algorithm with the matrices resulting from the previous step. After that, a feature vector of
each image based on the intensity of the two chromogens is determined. Finally, the images are
annotated using a systematically initialized k-means clustering algorithm with beta divergences. The
method clearly defines the initial boundaries of the categories, although some flexibility is added.
Experiments for the semi-quantitative scoring of images in five categories have been carried out
by comparing the results with the scores of four expert researchers yielding accuracies that range
between 76.60% and 94.58%. These results show that the proposed automatic scoring system, which
is definable and reproducible, produces consistent results.FEDER / Junta de Andalucía-Consejería de Economía y Conocimiento US-1264994Fondo de Desarrollo (FEDER). Unión Europea PGC2018-096244-B-I00, SAF2016-75442-RMinisterio de Economía, Industria y Competitividad (MINECO). España TEC2017- 82807-
Algorithms for nonnegative matrix factorization with the beta-divergence
This paper describes algorithms for nonnegative matrix factorization (NMF)
with the beta-divergence (beta-NMF). The beta-divergence is a family of cost
functions parametrized by a single shape parameter beta that takes the
Euclidean distance, the Kullback-Leibler divergence and the Itakura-Saito
divergence as special cases (beta = 2,1,0, respectively). The proposed
algorithms are based on a surrogate auxiliary function (a local majorization of
the criterion function). We first describe a majorization-minimization (MM)
algorithm that leads to multiplicative updates, which differ from standard
heuristic multiplicative updates by a beta-dependent power exponent. The
monotonicity of the heuristic algorithm can however be proven for beta in (0,1)
using the proposed auxiliary function. Then we introduce the concept of
majorization-equalization (ME) algorithm which produces updates that move along
constant level sets of the auxiliary function and lead to larger steps than MM.
Simulations on synthetic and real data illustrate the faster convergence of the
ME approach. The paper also describes how the proposed algorithms can be
adapted to two common variants of NMF : penalized NMF (i.e., when a penalty
function of the factors is added to the criterion function) and convex-NMF
(when the dictionary is assumed to belong to a known subspace).Comment: \`a para\^itre dans Neural Computatio
Electroencephalograph (EEG) signal processing techniques for motor imagery Brain Computer interface systems
Brain-Computer Interface (BCI) system provides a channel for the brain to
control external devices using electrical activities of the brain without using the
peripheral nervous system. These BCI systems are being used in various medical
applications, for example controlling a wheelchair and neuroprosthesis devices for
the disabled, thereby assisting them in activities of daily living. People suffering
from Amyotrophic Lateral Sclerosis (ALS), Multiple Sclerosis and completely locked
in are unable to perform any body movements because of the damage of the
peripheral nervous system, but their cognitive function is still intact. BCIs operate
external devices by acquiring brain signals and converting them to control
commands to operate external devices. Motor-imagery (MI) based BCI systems, in
particular, are based on the sensory-motor rhythms which are generated by the
imagination of body limbs. These signals can be decoded as control commands in
BCI application. Electroencephalogram (EEG) is commonly used for BCI applications
because it is non-invasive. The main challenges of decoding the EEG signal are
because it is non-stationary and has a low spatial resolution. The common spatial
pattern algorithm is considered to be the most effective technique for
discrimination of spatial filter but is easily affected by the presence of outliers.
Therefore, a robust algorithm is required for extraction of discriminative features
from the motor imagery EEG signals.
This thesis mainly aims in developing robust spatial filtering criteria which
are effective for classification of MI movements. We have proposed two approaches
for the robust classification of MI movements. The first approach is for the
classification of multiclass MI movements based on the thinICA (Independent
Component Analysis) and mCSP (multiclass Common Spatial Pattern Filter) method.
The observed results indicate that these approaches can be a step towards the
development of robust feature extraction for MI-based BCI system.
The main contribution of the thesis is the second criterion, which is based on
Alpha- Beta logarithmic-determinant divergence for the classification of two class
MI movements. A detailed study has been done by obtaining a link between the AB
log det divergence and CSP criterion. We propose a scaling parameter to enable a
similar way for selecting the respective filters like the CSP algorithm. Additionally,
the optimization of the gradient of AB log-det divergence for this application was
also performed. The Sub-ABLD (Subspace Alpha-Beta Log-Det divergence)
algorithm is proposed for the discrimination of two class MI movements. The
robustness of this algorithm is tested with both the simulated and real data from BCI
competition dataset. Finally, the resulting performances of the proposed algorithms
have been favorably compared with other existing algorithms
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