Ecology of the non-native snail Sinotaia cf quadrata (Caenogastropoda: Viviparidae). A study in a lowland stream of South America with different water qualities

Sinotaia quadrata is a snail native from Asia recorded for the first time in South America in 2009 in central Argentina. In 2015, this species was also found in a lowland stream with different water qualities. Our aims were to contribute to the knowledge of its population ecology and to compare the individuals from the two locations anatomically. Snails were searched at 6 sites, where physicochemical and hydraulic parameters were measured. Biological samples were also taken at two sites (S3 and S4) to study the population traits of S. cf quadrata (density, size structure, fecundity and sex ratio) and to assess the water quality through macroinvertebrates' biological indices (richness, diversity and IBPamp). Physicochemical and biological parameters allowed us classifying sites as "moderately polluted" (S3) and "heavily polluted" (S4). At S4, the population showed a lower density, larger individuals, higher fecundity and a scarce representation of young snails. The differences observed in the radula and mantle border of snails from the two geographical regions might be attributed to environmental differences. We conclude that this species is tolerant to a wide range of environmental variables which, along with its high fecundity and morphological plasticity, could allow this species to colonize neighbor streams.Fil: Ferreira, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Paz, Laura Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Rumi Macchi Z., Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Ocon, Carolina Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Altieri, Paula Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Rodrigues Capitulo, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentin

Selección de presas por el caracolero (Rostrhamus sociabilis) en cuerpos de agua permanentes y temporarios del centro de Argentina.

– En este estudio se analizó el patrón de selección de presas del caracolero (Rostrhamus sociabilis) sobre el caracol de agua dulce Pomacea canaliculata en la provincia de Buenos Aires, Argentina. Para esto se colectaron los restos presa de esta especie bajo las perchas de alimentación del caracolero y se colectaron individuos vivos de P. canaliculata en cinco cuerpos de agua temporarios y en tres lagunas. En cada localidad de muestreo, se comparó la distribución de frecuencias de los tamaños de los caracoles colectados de la población y de los caracoles predados por el caracolero. El caracolero seleccionó el tamaño de sus presas en todas las localidades; sin embargo, al patrón de selección difirió entre los diferentes tipos de ambientes. En las lagunas, el caracolero seleccionó presas de tamaño intermedio, pero en los cuerpos de agua temporarios seleccionó tanto las presas intermedias como las de mayor tamaño. A pesar de esto, el tamaño promedio de los caracoles predados en los cuerpos de agua temporarios fue 8–17 mm menor que en las lagunas. Las estimaciones de abundancia de caracoles (y, presumiblemente, su disponibilidad para los caracoleros) fueron mayores en los cuerpos de agua temporarios que en las lagunas. Las diferencias en la abundancia de presas y en la fisonomía del hábitat entre ambientes podrían ser responsables de las diferencias en el patrón de selección de presas observado.Prey selection by snail kites (Rostrhamus sociabilis) on freshwater apple snails (Pomacea canaliculata) was studied in permanent and temporary wetlands in Buenos Aires province, Central Argentina. Live individuals of P. canaliculata and prey remains of this species left under perches by snail kites were collected in five temporary and three permanent wetlands. Frequency distributions of size of live and preyed-upon snails were compared at each sampling locality. Although snail kites selected prey by size both in temporary and permanent wetlands, the pattern of prey selection differed between wetland types. Snail kites selected prey of intermediate size in permanent wetlands but in temporary wetlands they selected for intermediate and large-sized snails. In spite of this selection pattern, snail preyed by snail kites in temporary wetlands was on average 8–17 mm smaller than in permanent wetlands. Estimates of snail abundance (and presumably prey availability) were higher in temporary wetlands than in permanent wetlands. Differences in habitat physiognomy and in snail abundance between both types of habitat could be responsible for differences in the pattern of prey selection between wetland types.Fil: Mapelli, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Kittlein, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentin

Phenotypic evidence for local adaptation to heat stress in the marine snail Chlorostoma (formerly Tegula) funebralis

Southern California (USA) populations of the intertidal marine snail Chlorostoma (formerly Tegula) funebralis generally occupy warmer climates and are exposed to high air temperatures during low tides more often than northern California populations. Available genetic data suggest there is extensive gene flow across a broad range of C. funebralis populations, so it is unclear if populations can adapt to differences in local environments. To test for population-specific responses to heat stress, three phenotypic assays were performed on three northern and on three southern populations of C. funebralis, after acclimation to common-garden conditions in the laboratory. Thermal drop-down, heat stress mortality, and heat stress reattachment assays were designed to evaluate ecologically relevant phenotypic responses to heat stress; these assays assessed tolerance during, mortality following, and speed of recovery following heat stress. The latter two tests indicate that southern populations consistently suffer significantly lower mortality and recover significantly more quickly following heat stress compared to northern populations. Hierarchical cluster analysis of stress response data clearly identified northern California and southern California regional groupings of populations. Thus, these results indicate that southern populations have higher tolerance to heat stress than northern populations and suggest that adaptation to local environmental differences can evolve despite moderate potential for larval dispersal in this species. Accounting for intraspecific population variation in thermal tolerance may provide important insights for predicting how species distributions will respond to global warming. © 2013 Elsevier B.V

Cryptic diversity: Two morphologically similar species of invasive apple snail in Peninsular Malaysia

Invasive snails in the genus Pomacea have spread across Southeast Asia including Peninsular Malaysia. Their effects on natural and agricultural wetlands are appreciable, but species-specific effects are less clear because of morphological similarity among the species. Our objective was to establish diagnostic characteristics of Pomacea species in Malaysia using genetic and morphological criteria. The mitochondrial COI gene of 52 adult snails from eight localities in Peninsular Malaysia was amplified, sequenced, and analysed to verify species and phylogenetic relationships. Shells were compared using geometric morphometric and covariance analyses. Two monophyletic taxa, P. canaliculata and P. maculata, occurred in our samples. The mean ratio of shell height: aperture height (P = 0.042) and shell height: shell width (P = 0.007) was smaller in P. maculata. P. maculata co-occurred with P. canaliculata in five localities, but samples from three localities contained only P.canaliculata. This study is the first to confirm the presence of two of the most invasive species of Pomacea in Peninsular Malaysia using a molecular technique. P. canaliculata appears to be the more widespread species. Despite statistical differences, both quantitative and qualitative morphological characteristics demonstrated much interspecific overlap and intraspecific variability; thus, shell morphology alone cannot reliably verify species identity. Molecular techniques for distinguishing between these two highly invasive Pomacea species are needed to understand their specific ecological niches and to develop effective protocols for their management

Pomacea insularus (Gastropoda : Pilidae) : its control under the integrated pest management (IPM) concept

Three control measures of Pomace a insularus as parts of IPM (Integrated Pest Management) components were studied; firstly the development of plant molluscicides, secondly the use of fish as its biological control and thirdly its utilisation as quail (Coturnix coturnix japonica) feed. Leaf powder of yellow flame (Peltophorum pterocarpum) was found to be quite effective in killing the snails. The powder is water soluble (28% solubility) and yielded high extracts (25% of water extraction and 23% of methanol extraction). The LC₅₀ value of this powder solution is about 91 mg/L at 72 h. exposure, on two-week-old test snails. Saponins were the active compounds found in the yellow flame leaves. Kept in solution form, the molluscicide strength deteriorated after 30 days with toxicity level reduced to 34%. The toxicity of the molluscicide in the field trials was found to be twice lower than that of Tea Seed Cake (TSC) powder, a molluscicide used in Malaysia. The broadcasting application of 150 kg/ha of this leaf powder molluscicide in 15 cm deep rice field (equivalent to 100 mg/L) killed 100% of the adult snails in three days as compared to about 75 kg/ha (equivalent to 50 mg/L) of TSC. Study on the control of the snails using fishes revealed that black carp, Mylopharyngodon piceus and hybrid African catfish, Clarias sp. were good snail predators. In the laboratory trials the former was more vigorous, consuming at the rate of 60% of its body weight, within 24 h, while the latter consumed only 7%. Due to the shape and size of its mouth, black carps had greater ability in swallowing the snails than catfish. Young black .carp of 25 g in size could consume snails of up to about 1.0 em in shell length. There were high correlationships between the size of snails consumed and the size of fish and the mouth width, with the equation ofY = 0.26 Ln (X) + 0.16 ( r2 = 0.93) and of Y = 0. 4 Ln (X) + 1.25 (r2 = 0.93), respectively. Adult catfish (119 - 171 g) could only consume snails of up to 1.5 em shell length. Results from the release of catfish into the rice field showed a clear trend of a reduction in the snail population. Macroinvertebrates populations presence in the rice field were another source of food supply to the fish, thus enabling the fish to grow without being given supplementary food. Biological control of this snail using fish was, however, confronted with the problem of predators such as birds, crab, eel and otters. Snail meal contained high protein (32%) and mineral (26%). It could be a substitute for fish meal, meat and bone meal or soya bean meal as quail feed without having any effect on its growth performance. Birds fed with snail meal also performed as good as those given commercial feed. The performance indices such as average daily gain (ADG), feed conversion ratio (FeR) and carcass percentage of birds given snail meal protein was comparable with those given conventional protein source. A palatability test conducted had shown that meat of the bird fed with snail meal was well accepted by food panelists. Each control measure of snails that has been studied demonstrated promising results. Thus the implementation of the control measures could be exercised in the field integrally to achieve managable control of the population of Pomace a insularu

Dieta de Pomacea canaliculata (gastropoda: ampullariidae) en su hábitat natural basada en análisis de contenidos digestivos e isótopos estables

Pomacea canaliculata es una especie nativa de la Cuenca del Río de la Plata, actualmente considerada invasora en el Sur y Este de Asia donde fue introducida con fines comerciales y se ha convertido en la principal plaga de los cultivos de arroz de la región.Realizar más investigaciones en el hábitat natural de este organismo es necesario debido a que características fundamentales de su ecología trófica permanecen aún desconocidas. El objetivo de este trabajo fue estudiar la dieta de P. canaliculata en su hábitat natural mediante los análisis de contenidos digestivos e isótopos estables de 13C y 15N. Las muestras biológicas fueron tomadas desde noviembre de 2009 hasta diciembre de 2010 en el arroyo Carnaval, un arroyo pampeano característico. Las muestras para análisis de isótopos estables y posterior aplicación de modelos de mezcla fueron colectadas en noviembre de 2011. La aplicación de un diagrama de estrategia trófica permitió establecer una dieta generalista con elevado consumo de detritos, seguidos por plantas vasculares y algas. Los resultados del modelo de mezcla, sin embargo, indicaron que la contribución relativa a la dieta de todos los recursos basales evaluados (materia orgánica particulada fina y gruesa, epipelon y macrófitas acuáticas) fue similar (aproximadamente 40 %). Estos resultados evidenciaron una fuerte plasticidad dietaria para la especie.Pomacea canaliculata is native to Rio de la Plata basin, and it is invasive in most of South and Southeast Asia after accidental introduction during unsuccessful attempts to establish commercial aquaculture of this species, and being present, the number one pest of rice crops in the region. Investigations in its native habitat are still needed because fundamental issues of its trophic ecology remain unknown. The aim of this research was to study the diet of P. canaliculata in its natural habitat through gut content techniques and stable isotopes of 13C and 15N. Biological samples were taken from November 2009 to December 2010 in Carnaval stream, a characteristic Pampean stream. Samples for stable isotopes of 13C and 15N using of a mixing model were collected in November 2011. The trophic strategy diagram evinced a generalist diet with high consumption of detritus followed by the vascular plants and algae. The mixing model results indicated that the relative contribution to the diet was similar for all basal resources: fine and coarse particulate organic matter, epipelon and aquatic macrophytes (approximately 40 %). These results evidenced a strong dietary plasticity for the species.Fil: López Van Oosterom, Maria Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Ocon, Carolina Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Ferreira, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Rodrigues Capitulo, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentin

Elimination of Schistosomiasis Transmission in Zanzibar: Baseline Findings before the Onset of a Randomized Intervention Trial.

Gaining and sustaining control of schistosomiasis and, whenever feasible, achieving local elimination are the year 2020 targets set by the World Health Organization. In Zanzibar, various institutions and stakeholders have joined forces to eliminate urogenital schistosomiasis within 5 years. We report baseline findings before the onset of a randomized intervention trial designed to assess the differential impact of community-based praziquantel administration, snail control, and behavior change interventions. In early 2012, a baseline parasitological survey was conducted in ∼20,000 people from 90 communities in Unguja and Pemba. Risk factors for schistosomiasis were assessed by administering a questionnaire to adults. In selected communities, local knowledge about schistosomiasis transmission and prevention was determined in focus group discussions and in-depths interviews. Intermediate host snails were collected and examined for shedding of cercariae. The baseline Schistosoma haematobium prevalence in school children and adults was 4.3% (range: 0-19.7%) and 2.7% (range: 0-26.5%) in Unguja, and 8.9% (range: 0-31.8%) and 5.5% (range: 0-23.4%) in Pemba, respectively. Heavy infections were detected in 15.1% and 35.6% of the positive school children in Unguja and Pemba, respectively. Males were at higher risk than females (odds ratio (OR): 1.45; 95% confidence interval (CI): 1.03-2.03). Decreasing adult age (OR: 1.04; CI: 1.02-1.06), being born in Pemba (OR: 1.48; CI: 1.02-2.13) or Tanzania (OR: 2.36; CI: 1.16-4.78), and use of freshwater (OR: 2.15; CI: 1.53-3.03) showed higher odds of infection. Community knowledge about schistosomiasis was low. Only few infected Bulinus snails were found. The relatively low S. haematobium prevalence in Zanzibar is a promising starting point for elimination. However, there is a need to improve community knowledge about disease transmission and prevention. Control measures tailored to the local context, placing particular attention to hot-spot areas, high-risk groups, and individuals, will be necessary if elimination is to be achieved