Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway

Rgs2, a regulator of G proteins, lowers blood pressure by decreasing signaling through Gαq. Human patients expressing Met-Leu-Rgs2 (ML-Rgs2) or Met-Arg-Rgs2 (MR-Rgs2) are hypertensive relative to people expressing wild-type Met-Gln-Rgs2 (MQ-Rgs2). We found that wild-type MQ-Rgs2 and its mutant, MR-Rgs2, were destroyed by the Ac/N-end rule pathway, which recognizes Nα-terminally acetylated (Nt-acetylated) proteins. The shortest-lived mutant, ML-Rgs2, was targeted by both the Ac/N-end rule and Arg/N-end rule pathways. The latter pathway recognizes unacetylated N-terminal residues. Thus, the Nt-acetylated Ac-MX-Rgs2 (X = Arg, Gln, Leu) proteins are specific substrates of the mammalian Ac/N-end rule pathway. Furthermore, the Ac/N-degron of Ac-MQ-Rgs2 was conditional, and Teb4, an endoplasmic reticulum (ER) membrane-embedded ubiquitin ligase, was able to regulate G protein signaling by targeting Ac-MX-Rgs2 proteins for degradation through their N^α-terminal acetyl group

X-ray Spectroscopy of the Cluster of Galaxies Abell 1795 with XMM-Newton

The initial results from XMM-Newton observations of the rich cluster of galaxies Abell 1795 are presented. The spatially-resolved X-ray spectra taken by the European Photon Imaging Cameras (EPIC) show a temperature drop at a radius of $\sim 200$ kpc from the cluster center, indicating that the ICM is cooling. Both the EPIC and the Reflection Grating Spectrometers (RGS) spectra extracted from the cluster center can be described by an isothermal model with a temperature of $\sim 4$ keV. The volume emission measure of any cool component ($<1$ keV) is less than a few % of the hot component at the cluster center. A strong OVIII Lyman-alpha line was detected with the RGS from the cluster core. The O abundance and its ratio to Fe at the cluster center is 0.2--0.5 and 0.5--1.5 times the solar value, respectively.Comment: Accepted: A&A Letters, 2001, 6 page

XMM-{\em Newton} and FUSE Tentative Evidence for a WHIM filament along the Line of Sight to PKS~0558-504

We present a possible OVIII X-ray absorption line at $z=0.117 \pm 0.001$ which, if confirmed, will be the first one associated with a broad HI Ly$\beta$ (BLB: FWHM=$160^{+50}_{-30}$ km s$^{-1}$) absorber. The absorber lies along the line of sight to the nearby ($z=0.1372$) Seyfert 1 galaxy PKS~0558-504, consistent with being a WHIM filament. The X-ray absorber is marginally detected in two independent XMM-Newton spectra of PKS~0558-504, a long $\sim 600$ ks Guest-Observer observation and a shorter, $\sim 300$ ks total, calibration observation, with a combined single line statistical significance of 2.8$\sigma$ (2.7$\sigma$ and 1.2$\sigma$ in the two spectra, respectively). When fitted with our self-consistent hybrid-photoionization WHIM models, the combined XMM-{\em Newton} spectrum is consistent with the presence of OVIII K$\alpha$ at $z=(0.117 \pm 0.001)$. This model gives best fitting temperature and equivalent H column density of the absorber of log$T=6.56_{-0.17}^{+0.19}$ K, and logN$_H=(21.5 \pm 0.3) (Z/Z_{0.01\odot})^{-1}$ cm$^{-2}$. The statistical sigificance of this single X-ray detection is increased by the detection of broad and complex HI Ly$\beta$ absorption in archival FUSE spectra of PKS~0558-504, at redshifts $z=0.1183 \pm 0.0001$ consistent with the best-fitting redshift of the X-ray absorber. The single line statistical significance of this line is 4.1$\sigma$ (3.7$\sigma$ if systematics are considered), and thus the combined (HI+OVIII) statistical significance of the detection is of 5.0$\sigma$. The detection of both metal and H lines at a consistent redshift, in this hot absorbing system, allows us to speculate on its metallicity. By associating the bulk of the X-ray absorber with the BLB line detected in the FUSE spectrum at $z_{BLB}=0.1183 \pm 0.0001$, we obtain a metallicity of 1-4\% Solar.Comment: 37 pages, 12 figures, 3 Tables. Accepted for publication by the ApJ

A possible Chandra and Hubble Space Telescope detection of extragalactic WHIM towards PG 1116+215

(Abridged) We have analyzed Chandra LETG and XMM-Newton RGS spectra towards the z=0.177 quasar PG 1116+215, a sightline that is rendered particularly interesting by the HST detection of several OVI and HI broad Lyman-alpha absorption lines that may be associated with the warm-hot intergalactic medium. We performed a search for resonance K-alpha absorption lines from OVII and OVIII at the redshifts of the detected far-ultraviolet lines. We detected an absorption line in the Chandra spectra at 5.2 sigma confidence level at wavelengths corresponding to OVIII K-alpha at z=0.0911+-0.0004+-0.0005 (statistical followed by systematic error). This redshift is within 3 sigma of that of a HI broad Lyman-alpha of b=130 km/s at z=0.09279+-0.00005. We have also analyzed the available XMM-Newton RGS data towards PG 1116+215. Unfortunately, the XMM-Newton data are not suitable to investigate this line because of instrumental features at the wavelengths of interest. At the same redshift, the Chandra and XMM-Newton spectra have OVII K-alpha absorption line features of significance 1.5 sigma and 1.8 sigma, respectively. We also analyzed the available SDSS spectroscopic galaxy survey data towards PG 1116+215 in the redshift range of interest. We found evidence for a galaxy filament that intersects the PG 1116+215 sightline and additional galaxy structures that may host WHIM. The combination of HST, Chandra, XMM-Newton and SDSS data indicates that we have likely detected a multi-temperature WHIM at z=0.091-0.093 towards PG 1116+215.Comment: Accepted for publication in MNRA

Discovery of X-ray absorption features from the dipping low-mass X-ray binary XB 1916-053 with XMM-Newton

We report the discovery of narrow Fe XXV and Fe XXVI K alpha X-ray absorption lines at 6.65 and 6.95 keV in the persistent emission of the dipping low-mass X-ray binary (LMXB) XB 1916-053 during an XMM-Newton observation performed in September 2002. In addition, there is marginal evidence for absorption features at 1.48 keV, 2.67 kev, 7.82 keV and 8.29 keV consistent with Mg XII, S XVI, Ni XXVII K alpha and Fe XXVI K beta transitions, respectively. Such absorption lines from highly ionized ions are now observed in a number of high inclination (ie. close to edge-on) LMXBs, such as XB 1916-053, where the inclination is estimated to be between 60-80 degrees. This, together with the lack of any orbital phase dependence of the features (except during dips), suggests that the highly ionized plasma responsible for the absorption lines is located in a cylindrical geometry around the compact object. Using the ratio of Fe XXV and Fe XXVI column densities, we estimate the photo-ionization parameter of the absorbing material to be 10^{3.92} erg cm s^{-1}. Only the Fe XXV line is observed during dipping intervals and the upper-limits to the Fe XXVI column density are consistent with a decrease in the amount of ionization during dipping intervals. This implies the presence of cooler material in the line of sight during dipping. We also report the discovery of a 0.98 keV absorption edge in the persistent emission spectrum. The edge energy decreases to 0.87 keV during deep dipping intervals. The detected feature may result from edges of moderately ionized Ne and/or Fe with the average ionization level decreasing from persistent emission to deep dipping. This is again consistent with the presence of cooler material in the line of sight during dipping.Comment: 13 pages, accepted for publication in Astronomy and Astrophysic

A strong emission line near 24.8 angstrom in the X-ray binary system MAXI J0556--332: gravitational redshift or unusual donor?

We report the discovery of a strong emission line near 24.8 angstrom (0.5 keV) in the newly discovered X-ray binary system MAXI J0556-332 with the reflection grating spectrometer onboard the XMM-Newton observatory. The X-ray light curve morphology during these observations is complex and shows occasional dipping behavior. Here we present time- and rate-selected spectra from the RGS and show that this strong emission line is unambiguously present in all the XMM observations. The measured line center is consistent with the Ly-alpha transition of N VII in the rest frame. While the spectra contain imprints of absorption lines and edges, there appear to be no other significantly prominent narrow line due to the source itself, thus making the identification of the 24.8 angstrom line uncertain. We discuss possible physical scenarios, including a gravitationally redshifted O VIII Ly-alpha line originating at the surface of a neutron star or an unusual donor with an extremely high N/O abundance (>57) relative to solar, that may have produced this comparatively strong emission line.Comment: Accepted for publication in ApJ Letters. Uses aastex.cls, 4 figures, 2 tables, 15 page

An XMM-Newton view of the `bare' nucleus of Fairall 9

We present the spectral results from a 130 ks observation, obtained from the X-ray Multi-Mirror Mission-Newton (XMM-Newton) observatory, of the type I Seyfert galaxy Fairall 9. An X-ray hardness-ratio analysis of the light-curves, reveals a `softer-when-brighter' behaviour which is typical for radio-quiet type I Seyfert galaxies. Moreover, we analyse the high spectral-resolution data of the reflection grating spectrometer and we did not find any significant evidence supporting the presence of warm-absorber in the low X-ray energy part of the source's spectrum. This means that the central nucleus of Fairall 9 is `clean' and thus its X-ray spectral properties probe directly the physical conditions of the central engine. The overall X-ray spectrum in the 0.5-10 keV energy-range, derived from the EPIC data, can be modelled by a relativistically blurred disc-reflection model. This spectral model yields for Fairall 9 an intermediate black-hole best-fit spin parameter of $\alpha=0.39^{+0.48}_{-0.30}$.Comment: Accepted for publication in MNRAS. The paper contains 11 figures and 1 tabl

Low-energy absorption towards the ultra-compact binary 4U1850-087 located in the globular cluster NGC6712

We report the results of two XMM-Newton observations of the ultra-compact low-mass X-ray binary 4U1850-087 located in the galactic globular cluster NGC6712. A broad emission feature at 0.7keV was detected in an earlier ASCA observation and explained as the result of an unusual Ne/O abundance ratio in the absorbing material local to the source. We find no evidence for this feature and derive Ne/O ratios in the range 0.14-0.21, consistent with that of the interstellar medium. During the second observation, when the source was 10% more luminous, there is some evidence for a slightly higher Ne/O ratio and additional absorption. Changes in the Ne/O abundance ratio have been detected from another ultra-compact binary, 4U1543-624. We propose that these changes result from an X-ray induced wind which is evaporated from an O and Ne rich degenerate donor. As the source X-ray intensity increases so does the amount of evaporation and hence the column densities and abundance ratio of Ne and O.Comment: 9 pages, 6 figures, accepted for publication in Astronomy and Astrophysic

The High-Velocity Outflow of PG1211+143 May Not be That Fast

We analyze the X-ray spectrum of the quasar PG1211+143 observed with the CCD and grating spectrometers on board XMM-Newton. Using an ion by ion fitting model we find an outflow component of about 3000 km/s that includes absorption lines of K-shell and L-shell ions of the astrophysically abundant elements. We also identify and include in our model broad (FWHM = 6000 km/s) emission lines from H-like ions of C, N, O, and Ne, and He-like ions of O, Ne, and Mg. The outflow velocity we find is an alternative interpretation of the data and is in contrast with the ultra high velocity of ~24000 km/s reported previously for this object. Nevertheless, we can not completely rule out the presence of a high velocity component due to the poor signal-to-noise ratio of the data.Comment: 7 pages, 2 figures, emulateapj, accepted for publication in The Astrophysical Journa