Molecular diagnostic methods can prevent unnecessary rejection of organic seed lots

In some years, large amounts of organic seed lots are discarded due to the presence of high levels of seed-borne diseases. In barley it is especially the presence of the fungal species Pyrenophora teres (barley net blotch) and P. graminea (barley leaf stripe), which causes rejection of seed lots. A real-time PCR method for detection and quantification of P. graminea has been developed

Leaf stripe resistance of spring barley cultivars

Results of six years of screening trials clearly indicate that effective resistance against barley leaf stripe is available, also in modern cultivars. Among the spring barley cultivars that are currently most widely grown in Denmark, Cabaret, Troon, Sebastian, Justina and Brazil appear most resistant, but only Brazil combines a favourable resistance performance (= low mean and standard deviation of environment-adjusted leaf stripe incidence) with a high number of observations (= years of testing). However, the cultivars appearing most resistant over the years are relatively old ones such as Vada, Alabama, Odin and particularly Scarlett, which has been resistant in all years in which it was tested. Amongst the new cultivars, Marigold, Native and SW Immer appear to have the most promising resistance properties so far. However, they have only been tested in two years of which only one had reasonably high infection levels. The results furthermore indicate huge year-to-year variation in infection levels, presumably due to variation in environmental conditions related to leaf stripe infection

Tan spot disease of wheat: race characterization.

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Control of seed borne diseasees in organic seed propagation

Introduction The key control measure of plant diseases in organic agriculture is crop rotation, mixed cropping and moderate fertilization. A wide range of plant diseases can be controlled or minimized in this way. However, at least one group of plant diseases, the seed borne diseases, cannot. The seed borne diseases are not transmitted through the soil, and crop rotation is therefore an insufficient tool. Mixed cropping is impractical in seed propagation, where seed purity according to the seed legislation is imperative. The fertilization level primarily has an impact on facultative saprophytes, and not on the specialized seed pathogens. Seed borne diseases were the first plant pathogens to be controlled by pesticides. Heavy metals has been used as seed treatments for more than 200 years, and for almost 100 years, the seed borne diseases has been controlled exclusively and very effectively by chemical seed treatments. On this background, research in control of seed borne diseases has had practically no priority in research programs during the last century. Compared with other agricultural topics, the control of seed borne diseases in organic agricultural therefore suffers from the largest lack of knowledge, as we are 100 years behind in research. International seed legislation does with a few exceptions not define minimum quality standards for seed infections with pathogens, as seed sold on the international market normally are treated with fungicides. Surveys show that for some crops, the nationally recommended thresholds for seed pathogens are regularly exceeded in organic seed-lots, and some years the majority of organic seed lots are discarded due to seed borne diseases in propagation systems, where seed health is assessed on a routine basis. To ensure the availability of organic seed for the organic farmers, control measures for seed borne diseases are imperative, and an international system to ensure seed health in organic seed lots should be implemented. Methods to control seed borne diseases in organic agriculture exist. Resistant varieties exist in many cases, and could be used to a wider extent. Different heat treatment can control most seed borne diseases, and new technologies can make this opportunity practical to implement. Technology to separate seed exists, and could be used as a tool to promote the propagation of seed in mixed cropping systems to decrease plant pathogens, including seed pathogens in propagation. Heavy and large seed are generally less infected than small and light seed. The separation and removal of the latter can therefore reduce the infection level in a seed lot. Some seed amendments of natural origin can be used in organic agriculture to replace synthetic pesticides. Ongoing projects Agrologica is currently involved in several projects on control of seed pathogens. This includes 1)heat treatments of cereals by drum-dryer, (Pyrenophora teres, Tilletia tritici, Ascochyta pisi, Fusarium ssp) 2)heat treatments of vegetables seed with steam and ultrasound, (Altanaria radicina, A. petroselini, Cladosporium sp, Septoria Petro, Stemphylium ssp, Phoma lingam, Botrytis ssp, Xantomonas compestris) 3)seed dressings, including plant extracts, smoke, natural chemicals and biological control, 4)physical cleaning of seeds to remove pathogens and infected seeds from seed lots (Ustilago nuda, Pyrenophora graminea, P. teres, T. tritici, Fusarium ssp). 5)integrated control of common bunt (T. tritici) in spelta-wheat (Triticum spelta), 6)preventive cropping methods to reduce build-up of pathogenic fungi during propagation (mixed cropping, early harvest), 7)determining threshold values for organic cereals related to the susceptibility of the individual varieties (P. graminea, P. teres, T. tritici, Ascochyta ssp, Fusarium ssp). Conclusions and recommendations Research during the last two decades has shown that progress can be achieved and that solutions exist. Based on this, it can be concluded that seed borne diseases can be controlled in organic agriculture. However, extension and research to refine methods are urgently needed to do so

Screening for resistance to leaf stripe (Pyrenophora graminea) in barley

Resistance against leaf stripe (Pyrenophora graminea ) have been described in the literature, but only little is known about the resistance in modern varieties. Investigations have been started in co-operation with Danish breeders with the objective to ensure availability of healthy seed for pesticide-free and organic growing of cereals. The results so far show great variation in susceptibility to leaf stripe. Most of the tested varieties or lines were susceptible, but some varieties had a moderate level of resistance and a few were highly resistant. The test have been made with different populations collected from Denmark but only little is known about the virulence pattern in leaf stripe

Strategies for regulation of seed borne diseases in organic farming

The impact of seed borne diseases on organic cereal production in North-Western Europe is discussed as well as the potential of the possible control measures. It is concluded that it is essential for the organic seed production to put more focus on the control the seed borne diseases. Possible control measures exist, but only a few are used at present. Small seeds are statistically more infected by a number of seed borne diseases than larger seeds. Removal of these seeds by size and gravity separation could be used more frequently. Varieties resistant to the most significant seed borne diseases are available, and improved heat treatment techniques are being developed and are likely to be available for practice in the coming years. Some organic certifying bodies allow application of cupper-salts and bio-agents, but the use of these does to some extent conflict with the fundamental principles of organic diseases management